Genetically modified Lactococcus lactis producing a green fluorescent protein–bovine lactoferrin fusion protein suppresses proinflammatory cytokine expression in lipopolysaccharide-stimulated RAW 264.7 cells

Shigemori, Suguru; Namai, Fu; Yamamoto, Yuroku; Nigar, Shireen; Sato, Takashi; Ogita, Tasuku; Shimosato, Takeshi

doi:10.3168/jds.2017-12872

Cited by 13 publications

(6 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lipopolysaccharide (LPS) induces acute inflammation and endotoxic shock in experimental animals [ 3 , 11 , 12 , 13 ], and it is characterized by the production of cytokines and other mediators of inflammation. Therefore, LPS may be used to induce inflammation to evaluate the anti-inflammatory effect of fermented milk.…”

Section: Introductionmentioning

confidence: 99%

Milk Fermented by Specific Lactobacillus Strains Regulates the Serum Levels of IL-6, TNF-α and IL-10 Cytokines in a LPS-Stimulated Murine Model

et al. 2018

View full text Add to dashboard Cite

Studies report that metabolites, such as peptides, present in fermented milk with specific lactic acid bacteria, may regulate cytokine production and exert an anti-inflammatory effect. Hence, the cytokine regulatory effect of fermented milk by specific Lactobacillus strains was evaluated in a lipopolysaccharide (LPS)-stimulated murine model. From twelve strains, three (J20, J23 and J28) were selected for their high proteolytic and acidifying capacities in milk and used for the in vivo study. Three treatments (fermented milk, FM; pasteurized fermented milk, PFM; and its <10 kDa fractions, PFM10) were administrated daily for four weeks. After treatments, animals were induced to a systemic inflammation with LPS, and blood samples were collected 6 h post-LPS injection for cytokine analyses. Results showed that FM or PFM significantly (p > 0.05) reduced pro-inflammatory cytokine (IL-6 and TNF-α) concentrations and significantly increased anti-inflammatory (IL-10) cytokine concentrations in comparison to the control; also, pro-inflammatory cytokines were reduced for animals treated with PFM10 (p < 0.05). RP-HPLC-MS/MS analysis showed that water-soluble extracts (<10 kDa) from PFM with J28 presented 15 new peptides, which may be the metabolites involved in the cytokine regulatory effect of fermented milk.

show abstract

Section: Introductionmentioning

confidence: 99%

Milk Fermented by Specific Lactobacillus Strains Regulates the Serum Levels of IL-6, TNF-α and IL-10 Cytokines in a LPS-Stimulated Murine Model

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Lactoferrin can also have anti-inflammatory effects and reduce gut permeability. Several other studies also have demonstrated that lactoferrin reduced LPS-induced inflammation via suppression of TLR4/MyD88/NF-kB pathway [126][127][128]. In vitro studies have also demonstrated that bovine lactoferrin can increase the integrity of the epithelial intestinal barrier [129] It has been shown that a population in Okinawa, Japan with healthy plant-based foods has 9% lower risk of COVID-19 infection and 41% lower risk of severe COVID-19 [130,131].…”

Section: Nutraceuticals and Mineralsmentioning

confidence: 98%

The Role of the Gut-Lung Axis in COVID-19 Infections and Its Modulation to Improve Clinical Outcomes

Chen¹,

Vitetta²

2022

Front. Biosci. (Schol Ed)

View full text Add to dashboard Cite

The main entry point of SARS-CoV-2 is the respiratory tract and as such immune defence in this site determines if the virus will spillover to the systemic circulation and circulate and infect other major organs. The first line of mucosal immune defence is composed of mucins, an epithelial barrier, and immune cells in the nasal cavity. The lung immune defence is carried out by numerous alveoli. The lung microbiota is a key factor in determining the efficacy of lung mucosal immunity protection. The intestinal microbiota has been demonstrated to affect the severity of COVID-19. Gut dysbiosis is involved in hyperinflammation and multiple organ failure through communications with multiple organs. The gut lung axis could be the earliest axis affected in COVID-19. Through the gut-lung axis, gut dysbiosis can affect the pathogenesis of the lung in COVID-19. In this review, we summarise the effects that gut dysbiosis can progress on the lung, and the lung microbiota. The possible mechanisms and approaches for modulation are discussed.

show abstract

“…Therefore, regulatory mechanisms of OCT are urgently needed to improve prognosis of therapy as well as exploration of innovative therapeutic targets for intestinal mucositis. LPS is a virulent component from gram-negative bacteria that can induce excessive immune response through TLR4 [25]. Many TLRs including TLR2, TLR4, TLR5 and TLR9 are involved in intestinal mucositis [26].…”

Section: Discussionmentioning

confidence: 99%

Octreotide Alleviates Autophagy by Up-Regulation of MicroRNA-101 in Intestinal Epithelial Cell Line Caco-2

Li¹,

Wang²,

Gao³

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background: Intestinal mucositis is a common side-effect after anti-cancer therapy, which may greatly restrict the therapeutic effects. We aimed to explore the functional role of octreotide (OCT) in lipopolysaccharide (LPS)-induced autophagy of human intestinal epithelial cells as well as the underlying mechanisms. Methods: Cell viability and expression of proteins related to autophagy, AMPK and the mTOR pathway in LPS-treated Caco-2 cells were determined by CCK-8 assay and Western blot analysis, respectively. Effects of OCT on LPS-induced alterations as well as miR-101 expression were measured. Then, miR-101 was aberrantly expressed, and whether OCT alleviated LPS-induced autophagy through miR-101 was tested. Next, whether TGF-β-activated kinase 1 (TAK1) was involved in the regulation of miR-101 in LPS-induced autophagy was studied. Effects of OCT on monolayer permeability and tight junction level were analyzed via measuring transepithelial electrical resistance (TEER) and expression of tight junction proteins. Results: LPS reduced cell viability and increased autophagy through activating AMPK and inhibiting the mTOR pathway in Caco-2 cells. OCT alleviated LPS-induced alterations and repressed degradation of autophagosome. Then, we found that OCT affected autophagy through up-regulating miR-101 in LPS-treated cells. Moreover, miR-101-induced inactivation of AMPK and activation of the mTOR pathway in LPS-treated cells were reversed by inhibition of TAK1 phosphorylation. Finally, we found miR-101 was up-regulated in differentiated cells, and OCT protected the monolayer permeability and tight junction level. Conclusion: OCT repressed autophagy through miR-101-mediated inactivation of TAK1, along with inactivation of AMPK and activation of the mTOR pathway in LPS-treated Caco-2 cells.

show abstract

Genetically modified Lactococcus lactis producing a green fluorescent protein–bovine lactoferrin fusion protein suppresses proinflammatory cytokine expression in lipopolysaccharide-stimulated RAW 264.7 cells

Cited by 13 publications

References 26 publications

Milk Fermented by Specific Lactobacillus Strains Regulates the Serum Levels of IL-6, TNF-α and IL-10 Cytokines in a LPS-Stimulated Murine Model

Milk Fermented by Specific Lactobacillus Strains Regulates the Serum Levels of IL-6, TNF-α and IL-10 Cytokines in a LPS-Stimulated Murine Model

The Role of the Gut-Lung Axis in COVID-19 Infections and Its Modulation to Improve Clinical Outcomes

Octreotide Alleviates Autophagy by Up-Regulation of MicroRNA-101 in Intestinal Epithelial Cell Line Caco-2

Contact Info

Product

Resources

About