Milk Fermented by Specific Lactobacillus Strains Regulates the Serum Levels of IL-6, TNF-α and IL-10 Cytokines in a LPS-Stimulated Murine Model

Reyes-Díaz, Aline; Mata-Haro, Verónica; Hernández, Jesús; González‐Córdova, Aarón F.; Hérnández-Mendoza, Adrian; Reyes-Díaz, Ricardo; Torres-Llanez, María J.; Beltrán-Barrientos, Lilia M.; Vallejo-Córdoba, Belinda

doi:10.3390/nu10060691

Cited by 43 publications

(33 citation statements)

References 49 publications

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“…The reduced level of IL-6 among patients who consumed probiotic in our clinical trial translated similar findings in the recent experimental study on Lactobacillus fermentum J20, J23 and J28 in a murine model [26]. Interestingly, we observed similar findings among recruited patients who consumed placebo.…”

Section: Discussionsupporting

confidence: 90%

A randomized double-blind placebo-controlled trial of probiotics in post-surgical colorectal cancer

et al. 2019

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Background Our study aimed to determine the effect of probiotic consumption containing six viable microorganisms of 30 × 10 10 cfu Lactobacillus and Bifidobacteria strains for six months on clinical outcomes and inflammatory cytokines (TNF-α, IFN-γ, IL-6, IL-10, IL-12, IL-17A, IL-17C and IL-22) in patients with colorectal cancer. Methods Fifty-two patients with colorectal cancer were randomized at four weeks after surgery to receive either a placebo ( n = 25) or 30 billion colony-forming unit (CFU) of a mixture of six viable strains including 107 mg of Lactobacillus acidophilus BCMC® 12,130, Lactobacillus lactis BCMC® 12,451, Lactobacillus casei subsp BCMC® 12,313, Bifidobacterium longum BCMC® 02120, Bifidobacterium bifidum BCMC® 02290 and Bifidobacterium infantis BCMC® 02129 ( n = 27). Patients were instructed to take the product orally twice daily for six months. Infection status, diarrhea or hospital admission were recorded throughout the study. Blood was taken pre- and post-intervention to measure TNF-α, IFN-γ, IL-6, IL-10, IL-12, IL-17A, IL-17C and IL-22 using ELISA multiplex kit. Results The majority of cases (~ 70%) were in Duke’s C colorectal cancer for both groups. No surgical infection occurred and no antibiotics were required. Chemotherapy induced diarrhea was observed in both groups. Significant reduction in the level of pro-inflammatory cytokine, TNF-α, IL-6, IL-10, IL-12, IL-17A, IL-17C and IL-22 were observed in CRC patients who received probiotics as compared to pre-treatment level ( P < 0.05). However, there was no significant difference in the IFN-γ in both groups. Conclusions We have shown that probiotics containing six viable microorganisms of Lactobacillus and Bifidobacteria strains are safe to be consumed at four weeks after surgery in colorectal cancer patients and have reduced pro-inflammatory cytokines (except for IFN-gamma). Probiotic may modify intestinal microenvironment resulting in a decline in pro-inflammatory cytokines. Trial registration NCT03782428 ; retrospectively registered on 20th December 2018.

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Section: Discussionsupporting

confidence: 90%

A randomized double-blind placebo-controlled trial of probiotics in post-surgical colorectal cancer

et al. 2019

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“…The strains Lactobacillus fermentum J20 and J28, previously isolated from Mexican artisanal cheese, were selected on the basis that these bacteria have already exhibited both technological and probiotic potential [18], conjugated linoleic acid [19], and anti-inflammatory and immunomodulatory properties, by using models of colon inflammation with LPS [20] or Dextran Sulfate Sodium (DSS) [21], and healthy Wistar rats [18], respectively. Bacteria were grown in MRS (De Man, Rogosa, and Sharpe, Difco) broth (12 h, 37 °C).…”

Section: Methodsmentioning

confidence: 99%

Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study

et al. 2019

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The aim of this study was to evaluate the effect of milk fermented with Lactobacillus fermentum J20 (FMJ20) or J28 (FMJ28) on ameliorating indomethacin-induced inflammation. Twenty-eight male C57Bl/6 mice were divided into four experimental groups: indomethacin, indomethacin + FMJ20, indomethacin + FMJ28, and untreated (control). Groups were fed fermented milk for 15 days, followed by administration of indomethacin supplied in three sub-doses over experimental period. Body weight, and food consumption were recorded. Additionally, spleen, kidney, and liver were weighed, and the small intestine length was measured. The cytokines in serum (IL-2, IL-4, IL-6, IL-10, IL-17, IL-23 and TNFα) and in intestinal mucosa (IL-17 and IFNγ) were also determined. Compared to the control, all indomethacin-supplemented groups lost weight (~2.7 g; p < 0.05), but no changes were found in the organ-specific morphometry analysis. FMJ28 showed better results in attenuating serum and intestinal IL-17 levels. Furthermore, showed less epithelial cell loss and inflammatory infiltrates than the other indomethacin-treated groups. These results suggest that FMJ28 may be effective in reducing intestinal and systemic acute inflammation, specifically in mice.

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“…A recent study by Kanmani and Kim 16 reported that several types of lactic acid bacteria attenuated LPS induced negative regulators (A20, Tollip and SIGIRR) and pro-inflammatory cytokines (IL-6 and TNF-α) in HepG2 cells at 12 h, but not significantly altered the mRNA expression of TLR2 and TLR4. Additionally, Lactobabillus strains FM also found to suppress TNF-α and IL-6 expression in a LPS-infected murine model 63 .…”

Section: Discussionmentioning

confidence: 93%

The protective effects of enriched citrulline fermented milk with Lactobacillus helveticus on the intestinal epithelium integrity against Escherichia coli infection

El-Nezami

Shah

2020

Sci Rep

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This study examined the protective effects of citrulline enriched-fermented milk with live Lactobacillus helveticus ASCC 511 (LH511) on intestinal epithelial barrier function and inflammatory response in IPEC-J2 cells caused by pathogenic Escherichia coli. Five percent (v/v) of fermented milk with live LH511and 4 mM citrulline (5%LHFM_Cit-4mM) significantly stimulated the population of IPEC-J2 cells by 36% as determined by MTT assay. Adhesion level of LH511 was significantly increased by 9.2% when incubated with 5%LHFM_Cit-4mM and 5%LHFM_Cit-4mM reduced the adhesion of enterohemorrhagic (EHEC) and entero-invasive (EIEC) E. coli in IPEC-J2 cells by 35.79% and 42.74%, respectively. Treatment with 5%LHFM_Cit-4mM ameliorated lipopolysaccharide (LPS) from E. coli O55:B5 induced activated inflammatory cytokines expression (TNF-α, IL-6 and IL-8) and concentration (IL-6 and IL-8) and early apoptosis. It restored the transepithelial electrical resistance (TEER) and regulated the expression and distribution of tight junction (TJ) proteins (zonula occluden-1 (ZO-1), occludin and claudin-1), tolllike receptors (TLRs) (TLR2 and TLR4) and negative regulators of TLRs signalling pathway (A20 and IRAK-M). In conclusion, our findings suggested that 5%LHFM_Cit-4mM might have the positive effects on improving and maintaining the intestinal epithelial cell integrity and inflammatory response under both normal and pathogenic LPS-stimulated conditions.Probiotics are defined as live micro-organisms that contribute health-promoting effects 1 . These organisms have been extensively investigated for their health-promoting effects on intestinal tract and modulating the intestinal epithelial barrier functions by several mechanisms, such as prevention against pathogenic adhesion, reinforcement of tight junctions (TJ), protective effects against damage on TJ, suppression of inflammatory cytokines and anti-apoptotic effects 2 .Pathogenic infection occurs when pathogen adheres to host intestinal epithelium 3 . The infection of pathogenic Escherichia coli strains, such as enterohemorrhagic (EHEC) and enteroinvasive (EIEC) E. coli, begins with the colonization and adherence to the intestinal mucosal surface and thus causing damage to the host 4 . The ability of probiotics to adhere to the intestinal epithelial cells is considered as a possible mechanism contributing to the inhibition effect against pathogenic adhesion by competing the adhesion site or/and for nutrients 3 or producing antimicrobial agents 5 . Several Lactobacillus strains, such as L. rhamnosus GG, L. fermentum, L. acidophilus and L. plantarum, have been demonstrated to adhere to the intestinal mucus and markedly diminish the adhesion of Bacteroides vulgatus, Clostridium histolyticum, Enterobacter aerogenes and Staphylococcus aureus 3 . Impaired intestinal barrier functions following pathogenic infection include TJ dysfunction, including increased epithelial permeability and damages to epithelial structure, trigger of pro-inflammatory response and excessive apoptosis. With regard to...

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Milk Fermented by Specific Lactobacillus Strains Regulates the Serum Levels of IL-6, TNF-α and IL-10 Cytokines in a LPS-Stimulated Murine Model

Cited by 43 publications

References 49 publications

A randomized double-blind placebo-controlled trial of probiotics in post-surgical colorectal cancer

A randomized double-blind placebo-controlled trial of probiotics in post-surgical colorectal cancer

Milk Fermented with Lactobacillus fermentum Ameliorates Indomethacin-Induced Intestinal Inflammation: An Exploratory Study

The protective effects of enriched citrulline fermented milk with Lactobacillus helveticus on the intestinal epithelium integrity against Escherichia coli infection

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