General Principles of Wound Healing

Witte, Maria; Barbul, Adrian

doi:10.1016/s0039-6109(05)70566-1

Cited by 688 publications

(576 citation statements)

References 112 publications

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“…This histological profile is characteristic of the initial phase of the wound healing process, when micro-circulatory and cell changes occur, presenting vasodilatation, increase of vascular permeability, edema and neutrophils and monocytes migration 1,18,20,21 . During this stage of wound healing some characteristics of the proliferation phase can also be observed, such as presence of fibroblasts and endothelial cells that produce the granulation tissue 1,22 . Additionally, the fibrin-leukocyte crusts were present in greater length in the wounds on the 7th day in all groups studied.…”

Section: Histological Analysismentioning

confidence: 99%

Profile of wound healing process induced by allantoin

et al. 2010

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Purpose:To evaluate and characterize the wound healing process profile induced by allantoin incorporated in soft lotion oil/water emulsion using the planimetric and histological methods. Methods: Female Wistar rats (n=60) were randomly assigned to 3 experimental groups: (C) control group-without treatment; (E) group treated with soft lotion O/W emulsion excipients; (EA) group treated with soft lotion O/W emulsion containing allantoin 5%. The emulsions either containing or not allantoin were topically administered for 14 days and the wound area was evaluated by planimetry and by qualitative and quantitative histological analysis of open wound model. Results:The data which were obtained and analyzed innovate by demonstrating, qualitatively and quantitatively, by histological analysis, the profile of healing process induced by allantoin. The results suggest that the wound healing mechanism induced by allantoin occurs via the regulation of inflammatory response and stimulus to fibroblastic proliferation and extracellular matrix synthesis. Conclusion: This work show, for the first time, the histological wound healing profile induced by allantoin in rats and demonstrated that it is able to ameliorate and fasten the reestablishment of the normal skin. Key words: Wound Healing. Allantoin. Histology. Animal Experimentation. Rats. RESUMOObjetivo: Avaliar e caracterizar o perfil cicatricial induzido pela alantoína incorporada em uma emulsão óleo/água, sob os aspectos planimétrico e histológico. Métodos: Ratos Wistar fêmeas (n=60) foram agrupados aleatoriamente em três grupos experimentais grupo controle -sem tratamento (C); grupo tratado com emulsão pura (E); grupo tratado com emulsão contendo 5% de alantoína (EA). As emulsões contendo ou não alantoína foram administradas topicamente durante 14 dias e a área da ferida foi avaliada por planimetria e por análise histológica qualitativa e quantitativa em modelo de ferida aberta. Resultados: Na análise planimétrica não foi observado diferenças significativas entre os grupos experimentais. Os resultados da análise histológica sugerem que o mecanismo de cicatrização induzido pela alantoína ocorre via controle da resposta inflamatória e estímulos à proliferação fibroblástica e síntese de matrix extracelular de maneira mais intensa e rapidamente em relação aos grupos controles. Conclusão: Este trabalho mostra pela primeira vez o perfil histológico de cicatrização induzido pela alantoína em ratos, demonstrando ser capaz de melhorar e acelerar o processo de reconstituição da pele. Descritores: Cicatrização de Feridas. Alantoína. Histologia. Experimentação Animal. Ratos.

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Section: Histological Analysismentioning

confidence: 99%

Profile of wound healing process induced by allantoin

et al. 2010

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“…Macrophages were observed on the 2nd day, according to literature data [10][11][12][13]16,22 , reaching their highest levels after the 7th day and remaining high until the end of the study period. This finding differs from classic results, which show that macrophages disappear around the 10th day after mesh implantation 10-13,16,22. Lymphocytes were identified on the polypropylene mesh after the 2nd day, which is inconsistent with the findings of other studies, which indicate that they often appear in the wound on the 5th day after tissue injury 10 .…”

Section: Discussionmentioning

confidence: 59%

“…This study allowed to observe that neutrophils were present on the surgical wound up to the 21st day, which is a longer period than that described in the literature [10][11][12][13]22 , indicating persistence of the acute inflammatory process. Macrophages were observed on the 2nd day, according to literature data [10][11][12][13]16,22 , reaching their highest levels after the 7th day and remaining high until the end of the study period.…”

Section: Discussionmentioning

confidence: 70%

“…These findings are consistent with previously reported ones 15,23,24 . In the healing process classically described for skin wounds [10][11][12][13]22 which is the basis for understanding the repair process of other tissues, neutrophils are the first cells to reach the wounded tissue, appearing approximately 24 hours after injury and remaining there for 3 to 5 days. Macrophages migrate to the wounded area 48 to 96 hours after injury, persisting from the 3rd to the 10th day, constituting the major cell type involved in the control and regulation of wound healing before migration and replication of fibroblasts 10,11,16,22 .…”

Section: Discussionmentioning

confidence: 99%

“…The fibroplasia process consists of a harmonious and coordinated sequence of cellular and molecular events which interact to promote the repair and reconstruction of damaged tissue [10][11][12][13] . Its extent depends on the level of inflammatory activity, one of the healing stages [14][15][16] , and is a prerequisite for the reconstruction of a mechanically stable abdominal wall.…”

Section: Introductionmentioning

confidence: 99%

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Fibroplasia after polypropylene mesh implantation for abdominal wall hernia repair in rats

et al. 2009

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RESUMOObjetivo: Avaliar o tempo de fibroplasia em tela de polipropileno na correção de hérnias incisionais da parede abdominal, em ratos, através da quantidade de colágeno, correlacionando-o com a resposta inflamatória local. Métodos: Trinta e seis ratos machos da linhagem Wistar foram submetidos à ressecção longitudinal de um segmento músculo-aponeurótico e peritoneal (3x2 cm) da parede abdominal, seguida por reforço com tela de polipropileno, em forma de ponte sobre a aponeurose. Os animais foram distribuídos em seis grupos, de acordo com o tempo de fibroplasia a ser estudado (1, 2, 3, 7, 21 e 30 dias de pós-operatório). Após os prazos estabelecidos para estudo da fibroplasia, os animais foram submetidos à eutanásia, e a área de fixação da tela de polipropileno foi avaliada histologicamente quanto à reação inflamatória e à percentagem de colágeno pela técnica videomorfométrica assistida por computador. Resultados: Houve aparecimento de colágeno total junto à tela no 3º dia pós-implante, com aumento progressivo na sua proporção em todos os dias subseqüentes até o 21º dia, quando atingiu sua proporção máxima (p<0,001). A partir do dia 3, o colágeno III sofreu um aumento progressivo até o dia 21, quando atingiu sua proporção máxima (p<0,001), e no 30º dia apresentou uma redução significativa (p<0,001). O colágeno tipo I surgiu entre o 7º e o 21º dia, apresentou sua máxima proporção no 21º dia e manteve-se inalterado até o final do período de observação. A relação colágeno tipo I/tipo III aumentou progressivamente e inverteu-se no 30º dia de observação (p<0,001). Os neutrófilos foram identificados no 1º dia pós-implante, mantendo-se junto à tela até o 21º dia. Os macrófagos, gigantócitos e linfócitos foram identificados no 2º dia. Trinta dias após a implantação da tela, desapareceram os neutrófilos e mantiveram-se estáveis as proporções de macrófagos, gigantócitos e linfócitos (p<0,001). Conclusões: Os resultados do presente estudo evidenciaram a presença de colágeno no 3º dia pós-implante, com predomínio do colágeno tipo I ao final do período de observação. O prolongamento da resposta inflamatória da cicatrização e a persistência do processo inflamatório crônico junto à tela não interferiram no tempo da fibroplasia. Descritores: Parede Abdominal. Colágeno. Hérnia. Telas Cirúrgicas. Polipropilenos. Neutrófilos. Ratos. ABSTRACT Purpose:This study assessed the collagen deposition and correlated it with local inflammatory responses to evaluate the length of time required for fibroplasia when polypropylene meshes are used to repair incisional abdominal wall hernias in rats. Methods: Thirty-six male Wistar rats underwent longitudinal resection of a peritoneal and musculoaponeurotic tissue segment (3x2 cm) of the abdominal wall followed by defect reconstruction with polypropylene mesh bridging over aponeurosis. The animals were divided into 6 groups according to the time points for the analysis of fibroplasia: 1, 2, 3, 7, 21 and 30 days post-implantation. Animals were sacrificed at each time point, and the site where th...

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Transforming Growth Factor β3 Promotes Fascial Wound Healing in a New Animal Model

Tyrone

Marcus²,

Bonomo³

et al. 2000

Arch Surg

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Transforming growth factor ␤ 3 (TGF-␤ 3) promotes fascial wound healing in a new animal model, as measured by wound breaking strength, collagen deposition, and cellular proliferation. Design/Intervention: Bilateral, longitudinal incisions were made in the anterior rectus sheaths of 24 male New Zealand white rabbits. One incision was treated with 1 µg of TGF-␤ 3 ; the contralateral incision served as a control. The wounds were harvested at 1, 2, 3, 4, 6, and 8 weeks after creation ("wounding"). Main Outcome Measures: Wound tissue was tested for breaking strength using a tensiometer and processed for histological examination of collagen deposition and cellular proliferation at all time points after wounding. Collagen deposition and cellular proliferation were measured in histological cross sections of wounds with Masson trichrome staining and proliferating cell nuclear antigen immunohistochemistry, respectively. Results: At all time points after wounding, treatment with TGF-␤ 3 significantly increased the wound breaking strength (up to 138%) and collagen deposition (up to 150%) over the control group. Cellular proliferation was increased during the first 3 weeks after wounding (up to 147%), but returned to baseline levels by the fourth week. Conclusions: Transforming growth factor ␤ 3 promotes fascial wound healing. In this new animal model of fascial wound healing, TGF-␤ 3 increased fascia breaking strength, collagen deposition, and cellular proliferation. These results are similar to findings in cutaneous wound models and demonstrate, for the first time, a pharmacologic agent to accelerate fascial healing.

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General Principles of Wound Healing

Cited by 688 publications

References 112 publications

Profile of wound healing process induced by allantoin

Profile of wound healing process induced by allantoin

Fibroplasia after polypropylene mesh implantation for abdominal wall hernia repair in rats

Transforming Growth Factor β3 Promotes Fascial Wound Healing in a New Animal Model

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