Gene probe assays and their detection

Nickerson, Deborah A.

doi:10.1016/0958-1669(93)90031-q

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1994

2022

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Cited by 7 publications

(2 citation statements)

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“…Nonetheless, the majority of known disease-causing mutations are point mutations. Several recent reviews summarize principles and applications of methods to search for previously unidentified or to detect known mutations in specific genes (Landegren, 1992;Nickerson, 1993;Cotton, 1993). In the following we will limit ourselves to discussing methods suitable to detect previously characterized point mutations.…”

Section: Introductionmentioning

confidence: 99%

Detection of point mutations by solid-phase methods

Syvänen

Landegren

1994

Hum. Mutat.

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Several techniques exist that permit the efficient distinction among characterized DNA sequence variants. In this review we discuss a number of such analytic procedures. These techniques all take advantage of a variety solid supports to prepare and analyze reaction products. The described diagnostic principles are now being applied for the development of miniaturized assay formats, suitable for automated detection of large sets of sequences in clinical samples.

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Section: Introductionmentioning

confidence: 99%

Detection of point mutations by solid-phase methods

Syvänen

Landegren

1994

Hum. Mutat.

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“…Direct labeling with fluorophores such as FITC and cyanine dyes have also been reported. The current assay formats most often require hybridization on solid supports, electrophoretic migration, solid-phase capture, or HPLC separation for the detection of specific nucleic acid sequences (14). All the methods involving separation steps can be avoided by using a homogeneous detection system.…”

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confidence: 99%