Nucleic acid based sensors

Bier, Frank F.; Früste, J. P.

doi:10.1007/978-3-0348-9043-4_7

Cited by 8 publications

(1 citation statement)

References 86 publications

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“…The appropriate sequence is then amplified by a combination of the well-established reverse transcription and polymerase chain reaction. 4 This procedure for identifying oligonucleotide ligands is called SELEX (systematic evolution of ligands by exponential enrichment). Using this highly innovative approach, a recognition partner has been synthesized that possesses a 10 4 -fold higher affinity constant for theophylline than for the very similar caffeine molecule.…”

Section: Biomimetic and Synthetic Systemsmentioning

confidence: 99%

New Recognition Elements in Biosensing

Scheller

Kleinjung

Bier

et al. 1998

Annals of the New York Academy of Sciences

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The borderline between molecular recognition element (MRE) tools that are biological in nature and synthetic (organic) receptor molecules is no longer well definable; these two classes of recognition elements are merging. This is especially true for enzyme models, polymers imprinted by biomolecules, ionophores (which mimic the function of channels), and (synthetic) oligonucleotides. In this paper, we introduce three new examples of modified recognition elements for biosensing: (i) bienzyme conjugates for quantification of inhibitors of choline esterase; (ii) recognition of L-adenosine by high-affinity oligonucleotides (aptamers); (iii) catalyzed conversion of alkanes by hydroperoxides in the presence of porphyrin complexes. BIOTECHNOLOGICAL RECOGNITION ELEMENTSEnzymes and antibodies represent powerful tools that allow for sensitive and specific methods of detection and quantitation to be developed for a wide variety of substances.Genetic engineering allows for the optimization of the properties of biomacromolecules, such as stability and substrate specificity, by exchanging amino acids. Multifunctional proteins can be provided by fusing the structural genes. The latter ap-37

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Section: Biomimetic and Synthetic Systemsmentioning

confidence: 99%

New Recognition Elements in Biosensing

Scheller

Kleinjung

Bier

et al. 1998

Annals of the New York Academy of Sciences

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Aptamers

James¹

2000

Encyclopedia of Analytical Chemistry

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Aptamers

Gopinath¹

2011

Encyclopedia of Analytical Chemistry

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Aptamers are artificial nucleic acid ligands that can be generated against a wide range of targets including amino acids, drugs, proteins, and other molecules. They are isolated from complex libraries of synthetic nucleic acid by an iterative process of adsorption, recovery, and reamplification. They have potential applications in analytical sensing devices, target validation, and as therapeutic agents. Aptamers are now widely accepted as substitutes for antibodies, especially in the diagnostic front. Aptamers are approved as drugs for therapeutics, and several aptamers are under clinical trials with the ultimate aim for commercialization.

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Nucleic acid based sensors

Cited by 8 publications

References 86 publications

New Recognition Elements in Biosensing

New Recognition Elements in Biosensing

Aptamers

Aptamers

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