Aptamers

Gopinath, Subash C.B.

doi:10.1002/9780470027318.a1402.pub2

Cited by 7 publications

(6 citation statements)

References 228 publications

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“…Further information about the topics described in this article can be found in the Encyclopedia of Analytical Chemistry (67)(68)(69)(70)(71) In this sense, general articles describing the role of extraction techniques (67,68) and specific one devoted to antibodies (69) and aptamers (70,71) are recommended.…”

Section: Discussionmentioning

confidence: 99%

Selective Nanoparticles in Microextraction

Ríos-Gómez

Lasarte‐Aragonés

Cárdenas

et al. 2016

Encyclopedia of Analytical Chemistry

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Sorption capacity is a critical issue in microextraction techniques where the amount of sorbent is reduced to the low milligram range and even lower. In this context, the use of sorbents with a high superficial area is really desirable. Nanoparticles (NPs) fulfill this main requirement while, at the same time, provide the analyst with a wide variety of interaction chemistries that can be easily selected depending on the analytical problem under study. In addition, certain NPs present especial properties (e.g. superparamagnetism) that make them so attractive in the microextraction context. Focusing on sorption capacity is meaningless when complex samples, the usual situation in Bioanalysis, are processed as matrix components may overload the sorbent avoiding the extraction of the target analytes. In this scenario, selectivity then becomes a critical variable of concern. This article discusses the role of selectivity in microextraction and provides a general overview of the main coatings used to boost the selectivity of NPs. In particular, this article describes the use of two groups of coatings as selectivity enhancers. On the one hand, the use of synthetic polymers including molecularly imprinted polymers (MIPs) and restricted access materials (RAMs) is a well‐established alternative in analytical sciences. On the other hand, biopolymers, such as antibodies and aptamers, exploit biorecognition that is by far the most selective interaction. Both alternatives are described in detail in this article.

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Section: Discussionmentioning

confidence: 99%

Selective Nanoparticles in Microextraction

Ríos-Gómez

Lasarte‐Aragonés

Cárdenas

et al. 2016

Encyclopedia of Analytical Chemistry

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“…The aptamer is a recently emerged molecule generated from the randomized library of molecules by a SELEX method. Aptamers are single-stranded RNA or DNA molecules that can specifically bind to targets, including peptides, proteins, small molecules, carbohydrates, toxins, and whole cells [ 24 , 25 ]. Aptamers form various structures due to their tendency to form single-stranded loops and helices.…”

Section: Introductionmentioning

confidence: 99%

Carbon Material Hybrid Construction on an Aptasensor for Monitoring Surgical Tumors

Ma¹,

Gopinath

Lakshmipriya

et al. 2022

Journal of Analytical Methods in Chemistry

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Carcinoembryonic antigen (CEA) is a glycoprotein, one of the common tumor biomarkers, found at low levels in body fluids. Generally, overexpression of CEA is found in various cancers, including ovarian, breast, lung, colorectal, gastric, and pancreatic cancers. Since CEA is an important tumor biomarker, the quantification of CEA is helpful for diagnosing cancer, monitoring tumor progression, and the follow-up treatment. This research develops a highly sensitive sandwich aptasensor for CEA identification on an interdigitated electrode sensor. Carbon-based material was used to attach a higher anti-CEA capture aptamer onto the sensor surface through a chemical linker, and then, CEA was quantified by the aptamer. Furthermore, CEA-spiked serum was tested by using the immobilized aptamer, which was found to not affect the target validation. The limit of detection for CEA in PBS and serum is calculated from a linear regression graph to be 0.5 ng/mL with R2 values of 0.9593 and 0.9657, respectively, over a linear range from 0.5 to 500 ng/mL. This CEA quantification by the aptasensor can help diagnose various surgical tumors and monitor their progression.

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“…Aptamer is an oligonucleotide, either RNA or DNA, that generates from the mixed pool of library by using the steps with binding, separation, and amplification [6–8]. Aptamers are known as an artificial antibody, which can be used for various applications to replace antibody [9,10].…”

Section: Introductionmentioning

confidence: 99%

Sensitive determination of NT‐proBNP for diagnosing abdominal aortic aneurysms incidence on interdigitated electrode sensor

Qiu

Wang

Zhang

et al. 2020

Biotech and App Biochem

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Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health‐related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immediate treatment. Blood‐based biomarker helps to diagnose AAA and to monitor the condition after AAA surgery. N‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP) is a hormone produced in the heart in small quantities and increased when the heart needs to work harder. NT‐proBNP was proved to be strongly linked with AAA incidence. Moreover, quantifying the level of NT‐proBNP helps to determine the risk factors on cardiovascular system after the surgery. This work is quantifying the NT‐proBNP on interdigitated electrode sensor by using NT‐proBNP binding aptamer. The detection limit of NT‐proBNP was calculated as 1 pg/mL on a linear regression curve [y = 0.2148x + 0.8849; R² = 0.9049]. The linear range with dose‐dependent analysis was from 0.01 until 100 ng/mL. Moreover, the control experiment with complementary aptamer sequence did not show the current signal, specifying the detection of NT‐proBNP. This research benefits to identify the heart condition of patient after the removal of AAA.

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Aptamers

Cited by 7 publications

References 228 publications

Selective Nanoparticles in Microextraction

Selective Nanoparticles in Microextraction

Carbon Material Hybrid Construction on an Aptasensor for Monitoring Surgical Tumors

Sensitive determination of NT‐proBNP for diagnosing abdominal aortic aneurysms incidence on interdigitated electrode sensor

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