Europium Cryptate-Tethered Ribonucleotide for the Labeling of RNA and Its Detection by Time-Resolved Amplification of Cryptate Emission

The sensitivity of the lehn cryptand—europium and terbium (III) complexes to anions compared to a coordinatively saturated systems

“…Desirable features for such biological probes are: Literature reports on the europium complex 1 showed that it satisfies many of these criteria and assays involving europium complexes of the derivatized ligand 3 are commercially available [5]. However, the reported protocols for assays using these derivatives reveal a major drawback in its photophysical properties.…”

Review: immunoassays in DNA damage and instability detection

“…In the context of molecular interactions, nucleotides labeled with Europium cryptate serve as the new type of biotinylated labels for the RNA:DNA hybrid detection through measurement of FRET (fluorescence resonance energy transfer) signal. The FRET technique allows to examine structure and dynamics of macromolecule interactions such as proteins and its approaches in the range of 10-100 Å, in many solution conditions which is greatly beneficial for nucleic acid studies [56][57][58][59][60]. For the purpose of nucleic acid analysis (DNA hybridization and sequencing, PCR), the OLA (oligonucleotide ligation assay) method is known to be of a great use.…”

Development and Applications of Fluorescent Oligonucleotides

“…In the case of perfectly matched duplexes, the ligation reaction can be achieved with good yields, while it fails to occur if a mismatch is present at the junction of the two ONs. These assays have been developed in homogeneous reaction formats using FRET as detection methods with several classes of Fs: conventional short-lived Fs such as Cy5, rhodamine and fluorescein derivatives, lanthanide chelates and lanthanides cryptates [229,230]. Recently, attempts at developing methods enabling the detection of point mutations in unamplified genomic DNA have been reported [231].…”