GC-MS Determination of Bioactive Constituents of Hydrilla verticillata (L.f.) Royle. Collected from Unpolluted and Polluted Water Sources

Kensa, V. Mary; Neelamegum, R.

doi:10.9734/ajob/2016/30954

Cited by 7 publications

(6 citation statements)

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“…Another study suggested that in case of active IBD patients, feces soluble IgA and IgG level was significantly more than control. Moreover, the %IgAand IgG-coated bacteria increased in feces of Crohn's disease patients along with the terminal ileal and perianal lesions [22]. Another study reported that soluble PGN of S. aureus is capable of increasing murine spleen B-lymphocytes proliferation and release immunoglobulins in both in-vitro and in-vivo studies and thus, act as a good mitogen.…”

Section: Discussionmentioning

confidence: 97%

Gut function restoration by indigenous cow milk in gut inflammation by peptidoglycan from Staphylococcus aureus via regulating NF-kB

2022

Food Nutr J

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In IBD patients, it is difficult to find a satisfactory treatment due to lack of understanding of pathogenesis. Thus, the present study focuses on finding milk based treatment by comparing Indigenous (SW, TP and GIR), cross-bred (KF and KS) and exotic cow breed (HF) milk in inflammatory conditions like IBD induced by peptidoglycan from Staphylococcus aureus. Milk was provided to rats in human equivalent doses on daily basis orally via bottles. A significant increase in macrophage phagocytic activity in GIR and HF group, but a significant reduction in splenocyte proliferation in different milk treated group was found as compared to PGN group. A significant reduction in gut permeability in SW, GIR, KF and KS after 1 and 4h was observed. Based on above results, SW and KS group were selected for studying of inflammatory signaling in Caco-2 cells. There was reduction in relative mRNA expression of TLR-2, TLR-4, NF-kB, TNF-α and IL-6 in digested SW milk group in Caco-2 cells. Thus, Indigenous Sahiwal milk can be used as a protective remedy due to its gut integrity restoration effect via regulating NF-kB in inflammatory diseases.

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Section: Discussionmentioning

confidence: 97%

Gut function restoration by indigenous cow milk in gut inflammation by peptidoglycan from Staphylococcus aureus via regulating NF-kB

2022

Food Nutr J

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“…H. verticillata mengandung berbagai metabolit sekunder. Kensa dan Neelamegam [10] menyebutkan hasil skrining H. verticillata mengandung metabolit sekunder yaitu flavonoid, alkaloid, dan steroid. Penelitian lain menyebutkan hasil skrining H. verticillata mengandung fenol, glikosida, alkaloid, dan steroid [11].…”

Section: Pendahuluanunclassified

Aktivitas Antioksidan Isolat Steroid Hasil Kromatografi Lapis Tipis dari Fraksi n-Heksana Hydrilla verticillata

Fasya

Purwantoro

Ulya

et al. 2020

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Hydrilla verticillata is one of Allah SWT creations that potential as an antioxidant. This study’s objectives were to determine antioxidant activity and identify steroid isolates of n-hexane fraction of H. verticillata. The steroid compounds were extracted using methanol solvent. The methanol extract was hydrolyzed with HCl 2 N and partitioned using n-hexane. The n-hexane fraction was tested phytochemically and separated using thin-layer chromatography (TLC). The steroids compounds were identified using UV-Vis spectrophotometer, FTIR, and LC-MS/MS. The result showed that maceration extraction produced 5.14% yield, whereas n-hexane fraction produce 47.95% yield. Steroid separation through analytical TLC revealed that n-hexane: ethyl acetate (4:1) as the best eluant with 12 spots, while separation using preparative TLC yielded 19 spots. Steroid isolate from TLC preparative has antioxidant activity with EC50 of 5.109 ppm. Identification of steroid compounds using UV-Vis produced maximum wavelengths at 203.9 and 276 nm, while using FTIR indicated the presence of O-H group, geminal dimethyl, C=O, C=C, secondary C-OH, and =C-H (alkene) which might contain steroid compounds. The result of LC-MS/MS showed the presence of β-sitosterol. Hydrilla verticillata merupakan salah satu ciptaan Allah SWT yang memiliki potensi sebagai antioksidan. Penelitian ini bertujuan mengetahui aktivitas antioksidan dan mengidentifikasi senyawa steroid pada isolat hasil pemisahan fraksi n-heksana H. verticillata. Senyawa steroid diekstraksi menggunakan pelarut metanol. Ekstrak pekat metanol dihidrolisis dengan HCl 2 N dan dipartisi dengan n-heksana. Fraksi n-heksana hasil partisi diuji fitokimia dan dipisahkan senyawanya menggunakan KLT (Kromatografi Lapis Tipis). Identifikasi dilakukan menggunakan UV-Vis, FTIR, dan LC-MS/MS. Hasil penelitian menunjukkan ekstraksi maserasi menghasilkan rendemen 5,14%, sedangkan rendemen partisi n-heksana sebesar 47,95%. Pemisahan steroid menggunakan KLT analitik menunjukkan bahwa variasi eluen terbaik adalah n-heksana: etil asetat (4:1) dengan 12 noda, sedangkan pemisahan KLT preparatif menghasilkan 19 noda. Isolat steroid hasil KLT preparatif memiliki aktivitas antioksidan dengan EC50 5,109 ppm. Identifikasi senyawa steroid menggunakan UV-Vis menunjukkan bahwa panjang gelombang maksimum sebesar 203,9 dan 276 nm, sedangkan untuk identifikasi isolat steroid menggunakan FTIR menunjukkan gugus fungsi O-H, geminal dimetil, C=O, C=C, C-OH sekunder dan =C-H (alkena) yang diduga merupakan senyawa steroid. Hasil LC-MS/MS menunjukkan adanya senyawa steroid β-sitosterol.

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“…However, in the study of the composition of the some spe cies of the family Hydrocharitaceae, the screening test showed a positive result for the presence of saponins (Pal & Nimse 2006, Prabha & Rajkumar 2015, Mary Kensa & Neelamegam 2015, Kotelnaya & Alekhina 2016. In this regard, the purpose of our study was to specify the content of saponins (in terms of glycyrrhizic acid) in the phytomass of plants of species belonging to Hydrocharitaceae.…”

Section: Notes On the Saponins In The Plants Of The Family Hydrocharimentioning

confidence: 99%

Notes on the saponins in the plants of the family Hydrocharitaceae

Kotelnaya¹,

(Alekhina)²,

Ефремов³

et al. 2019

Bot Pac

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A B S T R A C TAccording to the available literature data, the absence of saponins and sapogenins in the composition could be typical for the representatives of the Hydrocharitaceae family. However, in the study of the component composition, the screening test for saponins gave a positive result. In this regard, studies have been carried out to determine the content of saponins (in terms of glycyrrhizic acid) in the phy to mass of some species of the Hydrocharitaceae family (Egeria densa, Elodea ca na den sis, Hydrilla verticillata, Нydrocharis morsus-ranae, H. dubia, Najas marina, Stratiotes aloi des, Vallisneria americana) from different regions of Eurasia. The highest amount of saponins is contained in the phytomass Hydrocharis dubia (5.1±0.3 %), H. morsusranae (3.6±0.5 %) and Stratiotes aloides (3.4±0.3 %), the smallest in Elodea canadensis (1.2±0.2 %). According to the content of saponins (in terms of glycyrrhi zic acid), the studied plants of the family Hydrocharitaceae could be divided into two groups: with the largest (more than 3 %), interesting as the raw material base, and the smallest (less than 3 %). On model species, seasonal changes in the content of saponins are confirmed, the content is statistically significantly diffe rent by 1.3 times. K e y w o r d s : Hydrocharitaceae, glycyrrhizic acid, triterpenoid saponins, composition, phytochemistry Р Е З Ю М Е Котельная Я. И., Алехина Е. А., Ефремов А. Н., Болотова Я. В., Гу сель никова М. В., Николаенко С., Тома Ц. Сапонины растений семейст ва Hydrocharitaceae. Согласно имеющимся литературным данным, для представителей семейства Hydrocharitaceae характерно отсутствие в их составе са по ни нов и сапогенинов. Однако, при исследовании компонентного состава скрининг-тест на сапонины дал положительный результат. В связи с этим были выполнены исследования по определению содержания сапонинов (по глицирризиновой кислоте) в фитомассе некоторых видов растений семейства (Egeria densa, Elodea canadensis, Hydrilla verticillata, Нydrocharis morsusranae, H. dubia, Najas marina, Stratiotes aloides, Vallisneria americana), полученных из различных районов Евразии. Установлено, что наибольшее количество сапонинов содержится в фитомассе Нydrocharis dubia (5,1±0,3 %), H. morsus-ranae (3,6±0.5 %) и Stratiotes aloides (3.4±0.3 %), наименьшее − в Elodea canadensis (1,2± 0,2 %). Исследованные растения семейства Hydrocharitaceae по содержанию са понинов (в пересчете на глицирризиновую кислоту) можно разделить на две группы: с наибольшим (более 3 %) -интересные с точки зрения сырье вой базы, и наименьшим (менее 3 %). На модельных видах подтверждены се зонные изменения содержания сапонинов, содержание статистически значимо отличается в 1,3 раза. Ключевые слова: Hydrocharitaceae, глицирризиновая кислота, тритерпеновые сапо нины, содержание, фитохимия

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GC-MS Determination of Bioactive Constituents of Hydrilla verticillata (L.f.) Royle. Collected from Unpolluted and Polluted Water Sources

Cited by 7 publications

References 10 publications

Gut function restoration by indigenous cow milk in gut inflammation by peptidoglycan from Staphylococcus aureus via regulating NF-kB

Gut function restoration by indigenous cow milk in gut inflammation by peptidoglycan from Staphylococcus aureus via regulating NF-kB

Aktivitas Antioksidan Isolat Steroid Hasil Kromatografi Lapis Tipis dari Fraksi n-Heksana Hydrilla verticillata

Notes on the saponins in the plants of the family Hydrocharitaceae

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