Gut function restoration by indigenous cow milk in gut inflammation by peptidoglycan from Staphylococcus aureus via regulating NF-kB

Abstract

In IBD patients, it is difficult to find a satisfactory treatment due to lack of understanding of pathogenesis. Thus, the present study focuses on finding milk based treatment by comparing Indigenous (SW, TP and GIR), cross-bred (KF and KS) and exotic cow breed (HF) milk in inflammatory conditions like IBD induced by peptidoglycan from Staphylococcus aureus. Milk was provided to rats in human equivalent doses on daily basis orally via bottles. A significant increase in macrophage phagocytic activity in GIR and HF group, but a significant reduction in splenocyte proliferation in different milk treated group was found as compared to PGN group. A significant reduction in gut permeability in SW, GIR, KF and KS after 1 and 4h was observed. Based on above results, SW and KS group were selected for studying of inflammatory signaling in Caco-2 cells. There was reduction in relative mRNA expression of TLR-2, TLR-4, NF-kB, TNF-α and IL-6 in digested SW milk group in Caco-2 cells. Thus, Indigenous Sahiwal milk can be used as a protective remedy due to its gut integrity restoration effect via regulating NF-kB in inflammatory diseases.