Gastroprotective effect of swallow root (Decalepis hamiltonii) extract: Possible involvement of H+–K+–ATPase inhibition and antioxidative mechanism

A novel phenol-bound pectic polysaccharide fromDecalepis hamiltoniiwith multi-step ulcer preventive activity

“…Nevertheless, in both cases the causes of severe ulcerations are depicted in the current study in addition to observations from other investigators [35,1] . Our earlier studies indicated that phenolic antioxidants were efficient in inhibiting upregulated H + , K + -ATPase and recovering the depleted levels of antioxidant and antioxidant enzymes [17] . The effect of SRPP on gastric ulcers induced by swim and ethanol stress was investigated in in vivo rat models.…”

“…+ , K + -ATPase in vitro Fresh sheep stomach was obtained from a local slaughterhouse at Mysore and an enzyme extract was prepared [17] . The enzyme extract was incubated with different fractions of swallow root polysaccharide, in a reaction mixture containing 16 mmol/L Tris buffer (pH 6.5).…”

“…SRPP has ability to inhibit steps 1-7 including the growth of H pylori in vitro (6). and immunohistological evaluation was done as described previously [17] . Equal weight of gastric tissue from animals of each group was homogenized using Tris-HCl buffer pH 7.4.…”

“…Among the majority of identified sources, flavonoids [11,31] , and occasionally polysaccharides, have frequently been implicated as antiulcer agents [12][13][14] . We previously reported on a nontoxic, edible antioxidant source [17] -Decalepis hamiltonii, a significant antiulcerogenic properties in vitro and in vivo. High levels of antioxidant properties, probably just little less than that found in green tea, with multiple compounds [32] may play a critical role in inhibiting oxidative induced mucosal damage in ulcers [33] .…”

Protective Effects of Genipin on Gastrointestinal Disorders

“…Acidity was determined by titration versus 0.1 N NaOH using phenol red as indicator, and pH was measured using pH meter. + -ATPase, the enzyme secreted during the final phase of gastric secretion, was measured as previously described by Naik et al 11) Swine gastric membrane protein determined by Bradford's law was tested with 300 µg protein and pre-incubated at 37°C for 1 h with 0.300 mL of a reaction mixture, containing 20 mM MgCl 2 , 20 mM ATP, 20 mM KCl, and 20 mM Tris-HCl (pH 7.4) and incubated at 37°C for 30 min. Another 0.3 mL mixture containing 4.5% ammonium molybdate and 30% trichloroacetic acid 0.3 mL was added and centrifuged at 1500×g for 10 min.…”