Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay

Sierra-Arguello, Yuli M.; Furian, Thales Quedi; Perdoncini, Gustavo; Moraes, H. L. S.; Salle, Carlos Tadeu Pippi; Rodrigues, Laura Beatriz; Santos, Luciana Ruschel dos; Gomes, Marcos José Pereira; Nascimento, Vladimir Pinheiro do

doi:10.1371/journal.pone.0199974

Cited by 25 publications

(24 citation statements)

References 24 publications

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“…[1][2][3][4] In Brazil, campylobacteriosis has been underdiagnosed and underreported; in this way, there is a paucity of studies about this pathogen. [13][14][15][16][17][18][19] The aims of this study were to use WGS to assess the phylogenetic relationship, to determine the AMR genotypes and to compare AMR with the results obtained by AST against four important antimicrobials in clinical use for 116 C. jejuni strains isolated from humans, animals, food, and the environment between 1996 and 2016 in Brazil.…”

Section: Discussionmentioning

confidence: 99%

“…Specifically, subclade A1 included 19 strains isolated from humans (10) and animals (9) between 1996 and 2009, in Sao Paulo and Rio de Janeiro States. Subclade A2 was composed of 30 strains isolated from humans (18), animals (9), and food 3…”

Section: Phylogenetic Analysismentioning

confidence: 99%

“…11,12 In Brazil, cases of campylobacteriosis have been underreported and underdiagnosed, and studies of C. jejuni isolates have been scarce. [13][14][15][16][17][18][19] In this way, additional studies that assess the AMR profiles and the molecular genotyping would help to assess the characteristics of C. jejuni strains isolated in Brazil.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Resistance Profiles and Phylogenetic Analysis of Campylobacter jejuni Strains Isolated in Brazil by Whole Genome Sequencing

Frazão

Cao

Medeiros

et al. 2020

Microbial Drug Resistance

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The objectives of this work were to use whole genome sequencing (WGS) to determine the antimicrobial resistance genotypes of 116 Campylobacter jejuni strains isolated in Brazil and to compare it with the results obtained by antimicrobial susceptibility testing (AST). In addition, WGS was used to uncover the phylogenetic relationship among those strains. Results: By AST, the C. jejuni strains resistant to ciprofloxacin, tetracycline, doxycycline, and erythromycin were 51 (44%), 41 (35.3%), 41 (35.3%), and 6 (5.2%), respectively. By WGS, the genes aph(3¢)III, aadE, bla OXA-449 , bla OXA-184 , bla OXA-61 , and tet(O) were detected in 6 (5.2%), 3 (2.6%), 1 (0.9%), 10 (8.6%), 55 (47.4%), and 44 (38%) strains, respectively. Fifty-four (46.6%) strains showed the mutation T86I in the gyrA gene, and four (3.4%) strains presented the mutation A2075G in the 23S rRNA gene. The correlation between AST and WGS was 100% for ciprofloxacin, 97.5% for tetracyclines, and 66.7% for erythromycin. The whole genome single nucleotide polymorphism (SNP) tree clustered the C. jejuni strains into two clades comprising strains that were highly related from different sources, places, and years. Conclusion: The high rates of C. jejuni strains resistant to ciprofloxacin and tetracyclines are of concern and may represent a public health problem. WGS has a potential to be a powerful tool for the prediction of resistance of antibiotics used to treat campylobacteriosis. The results obtained by whole genome SNP analysis suggested the potential for transmission between clinical and nonclinical sources and between human and animal sources over the course of 20 years in Brazil.

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Section: Discussionmentioning

confidence: 99%

Section: Phylogenetic Analysismentioning

confidence: 99%

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Antimicrobial Resistance Profiles and Phylogenetic Analysis of Campylobacter jejuni Strains Isolated in Brazil by Whole Genome Sequencing

Frazão

Cao

Medeiros

et al. 2020

Microbial Drug Resistance

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“…DNA was extracted by boiling method following the procedure described by [ 28 ], with slight modification as reported by [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

Molecular determination of genetic diversity among Campylobacter jejuni and Campylobacter coli isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR)

Igwaran

Okoh

2020

Infection Ecology & Epidemiology

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Consumption of contaminated meat, milk, and water are among the major routes of human campylobacteriosis. This study aimed to determined the genetic diversity of C. coli and C. jejuni isolated from meat, milk, and water samples collected from different locations. From the 376 samples (meat = 248, cow milk = 72, and water = 56) collected, a total of 1238 presumptive Campylobacter isolates were recovered and the presence of the genus Campylobacter were detected in 402 isolates, and from which, 85 and 67 isolates were identified as C. jejuni and C. coli respectively. Of which, 71 isolates identified as C. coli (n = 35) and C. jejuni (n = 36) were randomly selected from meat, milk, and water samples and were genotyped using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The digital images of the ERIC-PCR genotype were analyzed by GelJ v.2.0 software. The diversity and similarity of the isolates were assessed via an unweighted-pair group method using average linkages clustering algorithm. The results showed that the 36 C. jejuni strains separated into 29 ERIC-genotypes and 4 clusters while the 35 C. coli were delineated into 29 ERIC-genotypes and 6 clusters. The study revealed the genetic diversity among C. coli and C. jejuni strains recovered from different matrices characterized by Gelj.

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“…Template DNA for PCR assay was extracted following the process described by Sierra-Arguello et al [39] with slight modification. Briefly, colonies isolated from the blood agar plates were grown in 5 mL of Tryptone Soya Broth (TSB) for 48 h at 42 • C under microaerobic conditions in a 10% CO 2 incubator.…”

Section: Dna Extractionmentioning

confidence: 99%

Campylobacteriosis Agents in Meat Carcasses Collected from Two District Municipalities in the Eastern Cape Province, South Africa

Igwaran

Okoh

2020

Foods

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Raw meats are sometimes contaminated with Campylobacter species from animal faeces, and meats have repeatedly been implicated in foodborne infections. This study evaluated the prevalence, virulence genes, antimicrobial susceptibility patterns, and resistance gene determinants in Campylobacter species isolated from retailed meat carcasses. A total of 248 raw meat samples were collected from butcheries, supermarkets, and open markets; processed for enrichment in Bolton broth; and incubated at 42 °C for 48 h in 10% CO2. Thereafter, the broths were streaked on modified charcoal cefoperazone deoxycholate agar (mCCDA) plates and incubated at the same conditions and for the same amount of time. After incubation, colonies were isolated and confirmed by Polymerase chain reaction using specific oligonucleotide sequences used for the identification of the genus Campylobacter, species, and their virulence markers. The patterns of antimicrobial resistance profiles of the identified isolates were studied by disk diffusion method against 12 antibiotics, and relevant resistance genes were assessed by PCR. From culture, 845 presumptive Campylobacter isolates were obtained, of which 240 (28.4%) were identified as genus Campylobacter. These were then characterised into four species, of which C. coli had the highest prevalence rate (22.08%), followed by C. jejuni (16.66%) and C. fetus (3.73%). The virulence genes detected included iam (43.14%), cadF (37.25%), cdtB (23.53%), flgR (18.63%), and flaA (1.96%), and some of the isolates co-harboured two to four virulence genes. Of the 12 antibiotics tested, the highest phenotypic resistance displayed by Campylobacter isolates was against clindamycin (100%), and the lowest level of resistance was observed against imipenem (23.33%). The frequency of resistance genes detected included catll (91.78%), tetA (68.82%), gyra (61.76%), ampC (55%), aac(3)-IIa (aacC2)a (40.98%), tetM (38.71%), ermB (18.29%), tetB (12.90%), and tetK (2.15%). There is a high incidence of Campylobacter species in meat carcasses, suggesting these to be a reservoir of campylobacteriosis agents in this community, and as such, consumption of undercooked meats in this community is a potential health risk to consumers.

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Fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli from poultry and human samples assessed by PCR-restriction fragment length polymorphism assay

Cited by 25 publications

References 24 publications

Antimicrobial Resistance Profiles and Phylogenetic Analysis of Campylobacter jejuni Strains Isolated in Brazil by Whole Genome Sequencing

Antimicrobial Resistance Profiles and Phylogenetic Analysis of Campylobacter jejuni Strains Isolated in Brazil by Whole Genome Sequencing

Molecular determination of genetic diversity among Campylobacter jejuni and Campylobacter coli isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR)

Campylobacteriosis Agents in Meat Carcasses Collected from Two District Municipalities in the Eastern Cape Province, South Africa

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