Molecular determination of genetic diversity among Campylobacter jejuni and Campylobacter coli isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR)

Abstract

Consumption of contaminated meat, milk, and water are among the major routes of human campylobacteriosis. This study aimed to determined the genetic diversity of C. coli and C. jejuni isolated from meat, milk, and water samples collected from different locations. From the 376 samples (meat = 248, cow milk = 72, and water = 56) collected, a total of 1238 presumptive Campylobacter isolates were recovered and the presence of the genus Campylobacter were detected in 402 isolates, and from which, 85 and 67 isolates were identified as C. jejuni and C. coli respectively. Of which, 71 isolates identified as C. coli (n = 35) and C. jejuni (n = 36) were randomly selected from meat, milk, and water samples and were genotyped using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The digital images of the ERIC-PCR genotype were analyzed by GelJ v.2.0 software. The diversity and similarity of the isolates were assessed via an unweighted-pair group method using average linkages clustering algorithm. The results showed that the 36 C. jejuni strains separated into 29 ERIC-genotypes and 4 clusters while the 35 C. coli were delineated into 29 ERIC-genotypes and 6 clusters. The study revealed the genetic diversity among C. coli and C. jejuni strains recovered from different matrices characterized by Gelj.

Prevalence, antimicrobial resistance, virulence gene profile and molecular typing of Campylobacter species isolated from poultry meat samples

“…Additionally, high diversity was determined between C. jejuni and C. coli isolated from different raw poultry meat samples. This part of our findings was akin to those reported from South Africa (Igwaran & Okoh, 2020a ), Egypt (Ahmed et al., 2015 ) and India (Milton et al., 2015 ).…”

“…Diverse Polymerase Chain Reaction (PCR)‐based typing, such as PCR sequencing, PCR‐ribotyping and enterobacterial repetitive intergenic consensus PCR (ERIC‐PCR) have been developed for the molecular typing of Campylobacter spp. (Igwaran & Okoh, 2020a ). ERIC‐PCR technique is a simple tool used to differentiate bacteria strains isolated from diverse sources.…”

Evaluation of ERIC-PCR method for determining genetic diversity among Escherichia coli isolated from human and retail imported frozen shrimp and beef

“…Over the last two decades, several authors have used ERIC-PCR as a rapid molecular typing method for determining genetic relationships among several bacterial species isolated from food, environmental, and human samples. In this study, ERIC-PCR demonstrated excellent discrimination among unrelated E. coli strains, and this method is a useful DNA fingerprinting technique for distinguishing E. coli isolated from different sources (Ateba and Mbewe 2014;Dorneles et al 2014;Fendri et al 2013;Gautam et al 2022;Igwaran and Okoh 2020;Pakbin et al 2021;de Sa Guimaraes et al 2011;Szczuka and Kaznowski 2004). Our results indicate that imported frozen beef and shrimp products might become an important source of enteric pathogenic E. coli in Saudi Arabia.…”