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ABSTRACT:The qualities of the treated final effluents of a wastewater treatment plant located in a rural community of the Eastern Cape Province of South Africa were assessed over the duration of 12 months. Parameters measured include pH, temperature, electrical conductivity, salinity, turbidity, total dissolved solid, dissolved oxygen, chemical oxygen demand , nitrate, nitrite and orthophosphate levels and these were simultaneously monitored in the treated final effluents and the receiving watersheds using standard methods. Unacceptably, high levels of the assayed parameters were observed in many cases for chemical oxygen demand (7.5-248.5 mg/L), nitrate (1.82-13.14 mg/L), nitrite (0.09-1.3 mg/L), orthophosphate (0.07 -4.81 mg/L), dissolved oxygen (4.15-11.22 mg/L) and turbidity (3.68-159.06 NTU) during the study period and are severally outside the compliance levels of the South African Guidelines and World Health Organization tolerance limits for effluents intended for discharge through public sewers into receiving watersheds. The study has revealed that there was an adverse impact on the physico-chemical characteristics of the receiving watershed as a result of the discharge of inadequately treated effluents from the wastewater treatment facility. This poses a health risk to several rural communities which rely on the receiving water bodies primarily as their sources of domestic water. There is need for the intervention of appropriate regulatory agencies to ensure production of high quality treated final effluents by wastewater treatment facilities in rural communities of South Africa.

A bioflocculant-producing marine bacterium previously isolated from marine sediment of Algoa Bay was screened for flocculant production. Comparative analysis of 16S rDNA sequence identified the isolate to have 99% similarity to Virgibacillus sp. XQ-1 and it was deposited in the GenBank as Virgibacillus sp. Rob with accession number HQ537127. The bacterium produced biflocculants optimally in glucose (70.4%) and peptone (70.4%) as sole sources of carbon and nitrogen, alkaline pH (12) (74%); and the presence of Fe2+ (74%). Chemical analysis of the bioflocculant revealed it to be a polysaccharide.

Human enteric viruses are causative agents in both developed and developing countries of many non-bacterial gastrointestinal tract infections, respiratory tract infections, conjunctivitis, hepatitis and other more serious infections with high morbidity and mortality in immunocompromised individuals such as meningitis, encephalitis and paralysis. Human enteric viruses infect and replicate in the gastrointestinal tract of their hosts and are released in large quantities in the stools of infected individuals. The discharge of inadequately treated sewage effluents is the most common source of enteric viral pathogens in aquatic environments. Due to the lack of correlation between the inactivation rates of bacterial indicators and viral pathogens, human adenoviruses have been proposed as a suitable index for the effective indication of viral contaminants in aquatic environments. This paper reviews the major genera of pathogenic human enteric viruses, their pathogenicity and epidemiology, as well as the role of wastewater effluents in their transmission.

The agricultural ecosystem creates a platform for the development and dissemination of antimicrobial resistance, which is promoted by the indiscriminate use of antibiotics in the veterinary, agricultural, and medical sectors. This results in the selective pressure for the intrinsic and extrinsic development of the antimicrobial resistance phenomenon, especially within the aquaculture‐animal‐manure‐soil‐water‐plant nexus. The existence of antimicrobial resistance in the environment has been well documented in the literature. However, the possible transmission routes of antimicrobial agents, their resistance genes, and naturally selected antibiotic‐resistant bacteria within and between the various niches of the agricultural environment and humans remain poorly understood. This study, therefore, outlines an overview of the discovery and development of commonly used antibiotics; the timeline of resistance development; transmission routes of antimicrobial resistance in the agro‐ecosystem; detection methods of environmental antimicrobial resistance determinants; factors involved in the evolution and transmission of antibiotic resistance in the environment and the agro‐ecosystem; and possible ways to curtail the menace of antimicrobial resistance.

The widespread use of organochlorine pesticides (OCPs), essentially for the control of insects and the cultivation of food crops, has led to the pollution of ecosystems. Despite being banned several years ago in the developed world, extensive use remains ongoing on the African continent. This review summarizes the occurrence, distributions, sources, and trends of OCPs in seven environmental matrices (atmosphere, water, sediments, soils, biota, human fluids and food products) in Africa. Findings in this review revealed that α-HCH, β-HCH dichlorodiphenyltrichloroethane (DDTs), and endosulfans were the most persistent OCP residues in the African environment, particularly DDTs in breast milk samples occurring in levels above the WHO stipulated limits, thus indicating a call for concern. Also, there was paucity of data available on OCP concentrations in ambient air. Future research efforts should prioritize testing these pollutants in the atmosphere, especially in countries where they are used more frequently. While most POP analysis studies used gas chromatography coupled to electron capture detector or mass spectrometer, it is recommended that further studies should use more sensitive analytical techniques such as gas chromatography with tandem mass spectrometry (GC-MS/MS), or gas chromatography coupled to high-resolution mass spectrometry (GC-HRMS). These instruments allow for the detection of secondary and tertiary metabolites, especially those found in water, biota and food products, which are critical vectors of OCPs to human and animal bodies. Training of farmers and other domestic users on the handling of pesticides is proposed.

The incidence and spatial distribution of polycyclic aromatic hydrocarbons (PAHs) in the Buffalo River Estuary in the Eastern Cape Province of South Africa were assessed in this study. A total of 60 surface water and 19 sediment samples were collected from 5 sites of the estuary over a period of 6 months (December 2015 to May 2016). Extraction of PAHs from the water and sediment samples was achieved by using liquid–liquid and soxhlet extraction methods respectively, followed by column clean up with silica gel and quantification by gas chromatography–flame ionization detection. Individual PAH levels in the water and sediment samples ranged from not detected (ND) to 24.91 μg/L and ND to 7792 μg/kg, respectively. Total concentrations of the PAHs in the water and sediment samples varied as 14.91–206 μg/L and 1107–22,310 μg/kg in that order. Total levels of the contaminants were above the target values in the two matrices and were higher in summer than autumn. Although the noncarcinogenic risk of PAHs estimated in the water column through dermal absorption was very low compared with the target value, the carcinogenic risk determined was high for both adults and children. Similarly, benzo(a)pyrene and dibenzo(a,h)anthracene were found to be of higher carcinogenic and mutagenic risks in the sediments collected from the study area. Diagnostic ratios suggest that the target hydrocarbons are predominantly from pyrolytic sources. It therefore could be inferred that the water body is conspicuously polluted; hence, efforts should be made to control all the activities contributing to such magnitude of pollution at the sites.

The search for novel renewable products over synthetics hallmarked this decade and those of the recent past. Most economies that are prospecting on biodiversity for improved bio-economy favor renewable resources over synthetics for the potential opportunity they hold. However, this field is still nascent as the bulk of the available resources are non-renewable based. Microbial metabolites, emphasis on secondary metabolites, are viable alternatives; nonetheless, vast microbial resources remain under-exploited; thus, the need for a continuum in the search for new products or bio-modifying existing products for novel functions through an efficient approach. Environmental distress syndrome has been identified as a factor that influences the emergence of genetic diversity in prokaryotes. Still, the process of how the change comes about is poorly understood. The emergence of new traits may present a high prospect for the industrially viable organism. Microbial enzymes have prominence in the bio-economic space, and proteases account for about sixty percent of all enzyme market. Microbial keratinases are versatile proteases which are continuously gaining momentum in biotechnology owing to their effective bio-conversion of recalcitrant keratin-rich wastes and sustainable implementation of cleaner production. Keratinase-assisted biodegradation of keratinous materials has revitalized the prospects for the utilization of cost-effective agro-industrial wastes, as readily available substrates, for the production of high-value products including amino acids and bioactive peptides. This review presented an overview of keratin structural complexity, the potential mechanism of keratin biodegradation, and the environmental impact of keratinous wastes. Equally, it discussed microbial keratinase; vis-à-vis sources, production, and functional properties with considerable emphasis on the ecological implication of microbial producers and catalytic tendency improvement strategies. Keratinase applications and prospective high-end use, including animal hide processing, detergent formulation, cosmetics, livestock feed, and organic fertilizer production, were also articulated.