Abstract:A bioflocculant-producing marine bacterium previously isolated from marine sediment of Algoa Bay was screened for flocculant production. Comparative analysis of 16S rDNA sequence identified the isolate to have 99% similarity to Virgibacillus sp. XQ-1 and it was deposited in the GenBank as Virgibacillus sp. Rob with accession number HQ537127. The bacterium produced biflocculants optimally in glucose (70.4%) and peptone (70.4%) as sole sources of carbon and nitrogen, alkaline pH (12) (74%); and the presence of F… Show more
“…and Artrobacter spp. [92,93]. These findings imply that bacterial EPS effectively mediate flocculation and thus may be applied in large scale industrial processes, with particular reference to water and wastewater treatment.…”
Section: Future Prospects For Bacterial Epsmentioning
Majority of the polysaccharides used in foods are of plant, animal and algae origin. The exopolysaccharides (EPS) produced by food grade lactic acid bacteria (LAB) have gained much importance as biothickeners and texturizers in recent time. Several animal studies and in vitro tests are also suggestive of beneficial health effects associated with the regular consumption of EPS producing LAB. Such biopolymers are not synthesized in abundant amounts, which are a major factor impacting upon production cost and recovery time, considered as major constraints to full commercialization of these technologically important biopolymers. In current article, we reviewed prospective food and health applications of bacterial EPS.
“…and Artrobacter spp. [92,93]. These findings imply that bacterial EPS effectively mediate flocculation and thus may be applied in large scale industrial processes, with particular reference to water and wastewater treatment.…”
Section: Future Prospects For Bacterial Epsmentioning
Majority of the polysaccharides used in foods are of plant, animal and algae origin. The exopolysaccharides (EPS) produced by food grade lactic acid bacteria (LAB) have gained much importance as biothickeners and texturizers in recent time. Several animal studies and in vitro tests are also suggestive of beneficial health effects associated with the regular consumption of EPS producing LAB. Such biopolymers are not synthesized in abundant amounts, which are a major factor impacting upon production cost and recovery time, considered as major constraints to full commercialization of these technologically important biopolymers. In current article, we reviewed prospective food and health applications of bacterial EPS.
“…He et al [41] reported that glucose, sucrose and fructose were preferred carbon sources for REA-11 production, a bioflocculant produced by Corynebacterium glutamicum. It is well documented in the literature that many bioflocculant-producing microorganisms prefer organic carbon sources for optimum bioflocculant production [42]. However, the utilization of inorganic carbon sources for bioflocculant production is still scant in literature.…”
Section: Effect Of Carbon Source On Bioflocculant Productionmentioning
confidence: 99%
“…Nitrogen sources play a crucial role in the production of bioflocculants [42]. Different microorganisms utilize either organic or inorganic nitrogen sources, or both, to produce bioflocculants [43].…”
Section: Effect Of Nitrogen Source On Bioflocculant Productionmentioning
Abstract:A bioflocculant produced by a Bacillus species was assessed with regards to its physiochemical properties and flocculating efficiency. Identification of the bacteria through 16S rDNA sequencing revealed it to have 99% similarity to Bacillus pumilus strain ZAP 028. The optimum culture conditions for bioflocculant production by the bacterial strain were inoculum size of 4% (v/v), maltose as a sole carbon source, multiple nitrogen source (yeast extract, urea and ammonium sulfate) and medium initial pH 7. The bioflocculant was thermostable with high flocculating rate for kaolin suspension at low dosage 0.1 mg/mL over a wide pH range (3-11). Fourier-transform infrared (FTIR) spectroscopy analysis result of the purified bioflocculant showed that hydroxyl, amino and carboxyl groups were the main functional moieties in its molecular structure. The bioflocculant was composed of sugar (75.4%), protein (5.3%) and uronic acid (15.4%). Scanning electron microscopy (SEM) showed a dendritic bioflocculant structure and the energy dispersive X-ray spectroscopy (EDX) analysis revealed that the purified bioflocculant had weight fractions of elements as follows: 22.71% of C, 11.56% of N, 41.60% of O, 0.51% of S and 7.98% of P. The bioflocculant produced had strong flocculating activity and high thermal stability, which affords its utilization in industrial processes.
“…The result has been reported with bioflocculants production by Virgibacillus sp. Rob (Cosa et al 2011), Bacillus firmus (Salehizadeh and Shojaosadati 2002), and Enterobacter aerogene (Wenyu et al 2005). The glycosidase commonly present in the digestive systems of termite Nasutitermes takasagoensis degrades the cellulosic into glucose, while its activity was largely inhibited by the production of glucose (Tokuda et al 2009).…”
Bioflocculants are special high-molecular weight polymers produced by microorganisms. Despite the fact that several types of bioflocculants from different species of bacteria have been reported, there is a large gap in our knowledge regarding the molecular machine responsible for the production of bioflocculants. To investigate genes involved in bioflocculant synthesis, a fosmid library was generated from Bacillus licheniformis genomic DNA and screened for the production of bioflocculant. Four positive clones with distinct flocculation were isolated by a two-pooling scheme. The clone with 662 U ml(-1) flocculating activity was sequenced. As a result, a 30-kb fragment with 26 hypothetical genes was identified in the bioflocculant-producing clone. Most of the predicted proteins encoded by the inserted genes showed significant homology with enzymes involved in the biosynthesis of polysaccharide. Based on these homologies, a biosynthesis pathway and two gene clusters involved in the production of the polysaccharide bioflocculant were proposed with the integration of functional descriptions of individual genes by metabolic databases, and a glucose-sensitive glycosidases was predicted. This research supplied significant data for potential application of bioflocculant-producing strains in wastewater refining and industrial downstream treatments.
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