Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends

Ncube, N S; Afolayan, A. J.; Okoh, Anthony I.

doi:10.5897/ajb07.613

Cited by 590 publications

(506 citation statements)

References 57 publications

(93 reference statements)

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“…Previously, in many studies of similar kinds, ethanol and methanol are used as extractants, however, it could not demonstrate the greatest sensitivity in yielding antimicrobial compounds [22] . For this reason in the present study five extractants based on their different degree of polarity and solubility, i.e., hexane, dichloromethane, ethyl acetate, methanol and water were used to obtain maximum active compounds in the extracts.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity of leaves extracts of Trifolium alexandrinum Linn. against pathogenic bacteria causing tropical diseases

Khan

Ahmed

Shukla

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

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Section: Discussionmentioning

confidence: 99%

Antibacterial activity of leaves extracts of Trifolium alexandrinum Linn. against pathogenic bacteria causing tropical diseases

Khan

Ahmed

Shukla

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

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“…The combination of innovative and conventional antimicrobial compounds is being put into practice to improve both the prophylactic and therapeutic efficacy of some drugs and lower drug dosage, thus reducing toxic side effects (Ncube et al 2008;Wei et al 2011).…”

Section: Synergism Studiesmentioning

confidence: 99%

New trends in peptide-based anti-biofilm strategies: a review of recent achievements and bioinformatic approaches

2012

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Antimicrobial peptides (AMPs) have a broad spectrum of activity and unspecific mechanisms of action. Therefore, they are seen as valid alternatives to overcome clinically relevant biofilms and reduce the chance of acquired resistance. This paper reviews AMPs and anti-biofilm AMP-based strategies and discusses ongoing and future work. Recent studies report successful AMP-based prophylactic and therapeutic strategies, several databases catalogue AMP information and analysis tools, and novel bioinformatics tools are supporting AMP discovery and design. However, most AMP studies are performed with planktonic cultures, and most studies on sessile cells test AMPs on growing rather than mature biofilms. Promising preliminary synergistic studies have to be consubstantiated and the study of functionalized coatings with AMPs must be further explored. Standardized operating protocols, to enforce the repeatability and reproducibility of AMP anti-biofilm tests, and automated means of screening and processing the ever-expanding literature are still missing.

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“…The yield in this study was rather low, although we used the extraction method specific to GAGs or GAGpeptides (Nakano et al, 2000). This is probably due to the naturally low occurrence of GAGs in these mushrooms, or their geographic origin and phytochemical content (Ncube et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Detection of a Non-animal Source of Glycosaminoglycans from Edible Mushrooms in Northern Thailand

Choocheep¹,

Nathip²

2018

CMUJNS

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Several kinds of cultivated or local edible mushrooms exist in northern Thailand. While their nutritional features have been extensively analyzed, the presence of phytochemicals and glycosaminoglycans has yet to be investigated. Thus, in this study, we examined the presence of phytochemicals and glycosaminoglycans from 10 cultivated or local mushrooms: milk white russula, hygroscopic earthstar, log white fungi, log black fungi, shitake mushroom, rosy russula, sajor-caju mushroom, Jew's ear, bolete, and straw mushroom. Phytochemical analysis revealed the presence of carbohydrates, amino acids, and flavonoids in the extracts from most of the mushrooms. We detected glycosaminoglycans in all of the extracts using dimethylmethylene blue (DMMB) dye-binding assay and UVVis spectrophotometry. Hygroscopic earthstar contained the most glycosaminoglycans and sajor-caju mushroom the least. However, we were unable to distinguish the types of glycosaminoglycans, which should be considered for future study. In summary, the phytochemicals found here are crucial non-animal dietary sources of carbohydrates, amino acids, and antioxidants. In addition, the glycosaminoglycans detected in the mushroom extracts in this study potentially offer a non-animal source of glycosaminoglycans that may have clinical applications, apart from their nutritive value.

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Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends

Cited by 590 publications

References 57 publications

Antibacterial activity of leaves extracts of Trifolium alexandrinum Linn. against pathogenic bacteria causing tropical diseases

Antibacterial activity of leaves extracts of Trifolium alexandrinum Linn. against pathogenic bacteria causing tropical diseases

New trends in peptide-based anti-biofilm strategies: a review of recent achievements and bioinformatic approaches

Detection of a Non-animal Source of Glycosaminoglycans from Edible Mushrooms in Northern Thailand

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