Fluorometric Assays

Causality-based diagnosis of histamine-related cardiorespiratory disturbances in surgical patients

“…Plasma histamine levels were determined by the fluorometricfluoroenzymatic method [4] and clinical signs and symptoms were recorded. Calculation of the performance criteria of a test is optimal, if the prevalence of the disease equals approximately 50%.…”

Adverse reactions to drugs: in vitro studies with isolated cells

“…In the clinical setting, blood samples can be taken during the reaction and histamine measured in the plasma samples. It has, however, to be remembered that the half life of histamine is short and that extreme care must be taken in the processing of blood samples to avoid contamination with basophil-derived histamine (see e. g. [2] for details of plasma preparation etc.). In order to investigate reactions in a series of patients, obviously the controlled clinical trial setting is much more appropriate (see e. g. [3]).…”