Fluorescence properties of curcumin-loaded nanoparticles for cell tracking

Abstract

BackgroundPosttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems.Materials and methodsIn the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done.ResultsTransplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking.ConclusionThis approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies.

Synthesis of nano-fibers containing nano-curcumin in zein corn protein and its physicochemical and biological characteristics

“…Next to the even distribution of Nano-CUR throughout the nanofibers, we have confirmed their lack of accumulation by observing the uniform fluorescent signals. Various studies have reported the fluorescent properties of curcumin 75 , 76 , which can be also detected by the usage of UV illumination or confocal laser scanning microscope (CLSM) 68 . Furthermore, our study has displayed the capability of oil/water Nano-CUR in exhibiting the fluorescent signals in zein nanofibers, which is indicative of their high compatibility with zein polymer, the formation of a homogeneous solution even in the course of spinning, and confirming the stability of zein/Nano-CUR homogenous solution during the electrospinning process.…”

Nanostructured Cellulose–Gellan–Xyloglucan–Lysozyme Dressing Seeded with Mesenchymal Stem Cells for Deep Second-Degree Burn Treatment

“…Membranes were dehydrated with crescent ethanol concentrations (30%, 50%, 70%, 90%, and 100% twice) for 10 minutes at each concentration, then critical point and gold coating were obtained. 35 Images were obtained with scanning electron microscopy (SEM; JSM-6360LV; Jeol, Tokyo, Japan) for analysis of integration between cells and membranes and cell morphology on membrane surfaces.…”

Stem Cell Therapy: Curcumin Does the Trick

“…Transplanted CNPs‐ADMSCs were obviously recognized and showed potential advantage when utilized in cell tracking. This method may have wide applications in labeled‐transplanted cell modeling and in developing improved strategies for stem cell therapy (Mogharbel et al, ).…”

“…Chronic exposure to arsenite disrupts adult stem cell homeostasis. Curcumin can prohibit this condition by different mechanisms, including (1) its interaction with Keap‐1, leading to transfer of transcription factor Nrf2 into nucleus to induce the expression of detoxifying enzymes; (2) an increase in GSH biosynthesis; and (3) the resultant toxicant disposition (Mogharbel et al, ). iAs: inorganic arsenic, GSH: glutathione.…”