Fluorescence-based surface magnifying chromoendoscopy and optical coherence tomography endoscope

Wall, R. Andrew; Barton, Jennifer K.

doi:10.1117/1.jbo.17.8.086003

Cited by 18 publications

(4 citation statements)

References 22 publications

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“…We have combined OCT and SMC into an endoscope previously described in detail . Briefly, the fluorescence‐based SMC utilizes a 638 nm laser diode as a light source, which can excite methylene blue fluorescence.…”

Section: Methodsmentioning

confidence: 99%

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Dual optical modality endoscopic imaging of cancer development in the mouse colon

et al. 2014

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Background and Objective We utilize a miniature, dual-modality endoscope that combines fluorescence-based surface magnifying chromoendoscopy (SMC) and optical coherence tomography (OCT) to follow the anatomical changes that occur during adenoma development in the mouse colon. Materials and Methods Twenty-five mice were treated with the carcinogen azoxymethane (AOM) to induce tumor development in the distal colon, or were treated with saline as control, and were imaged over six months. OCT detects adenoma number with high sensitivity and specificity and can measure lesion size. In methylene blue-lavaged colons, SMC detects changes in the colonic crypts. SMC images of control mouse colons exhibit reticulated patterns of crypts of equal size, forming either a dot or honeycomb pattern. Results Images of AOM-treated colons show mild crypt irregularities even in grossly normal tissue. Images of small to medium adenoma exhibit larger crypts, more intense signal, and irregular spacing whereas those of large adenoma have heterogeneous, intense signal and loss of crypt structure. Conclusions The combination of OCT and SMC permits the detection of neoplastic events from the earliest stages of crypt irregularities before gross tissue changes are noted, through to measuring the growth of protruding adenoma.

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Section: Methodsmentioning

confidence: 99%

“…OCT can be used to image human colonic crypts , and while our system has the capability to resolve mouse colonic crypts, the limited contrast of the very small crypts in the mouse precludes reliable imaging with OCT. Therefore, SMC is a complementary modality as it can visualize these structures with high resolution and contrast .…”

Section: Introductionmentioning

confidence: 99%

Dual optical modality endoscopic imaging of cancer development in the mouse colon

et al. 2014

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“…Side-viewing needle probes with diameters of a few hundred microns have also been demonstrated with OCT only 28 and using a dual-clad fiber for OCT and single-point fluorescence detection. 29 Other endoscopes of a few millimeters in diameter have combined various implementations of fluorescence imaging with OCT. [30][31][32][33] None of these examples combines widefield fluorescence imaging and OCT into a submillimeter diameter package. Furthermore, none of these examples includes UV excitation to use MFI for wide-field autofluorescence detection.…”

Section: Introductionmentioning

confidence: 99%

Ultraminiature optical design for multispectral fluorescence imaging endoscopes

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Black²,

Utzinger

et al. 2017

J. Biomed. Opt

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A miniature wide-field multispectral endoscopic imaging system was developed enabling reflectance and fluorescence imaging over a broad wavelength range. At 0.8-mm diameter, the endoscope can be utilized for natural orifice imaging in small lumens such as the fallopian tubes. Five lasers from 250 to 642 nm are coupled into a 125 - ? m diameter multimode fiber and transmitted to the endoscope distal tip for illumination. Ultraviolet and blue wavelengths excite endogenous fluorophores, which can provide differential fluorescence emission images for health and disease. Visible wavelengths provide reflectance images that can be combined for pseudo-white-light imaging and navigation. Imaging is performed by a 300 - ? m diameter three-element lens system connected to a 3000-element fiber. The lens system was designed for a 70-deg full field of view, working distance from 3 mm to infinity, and 40% contrast at the Nyquist cutoff of the fiber bundle. Measured performance characteristics are near design goals. The endoscope was utilized to obtain example monochromatic, pseudo-white-light, and composite fluorescence images of phantoms and porcine reproductive tract. This work shows the feasibility of packaging a highly capable multispectral fluorescence imaging system into a miniature endoscopic system that may have applications in early detection of cancer.

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“…The current imaging modalities that exist with these properties are optical coherence tomography (OCT), reflectance confocal microscopy, and fluorescence spectroscopy and fluorescence confocal microscopy. OCT has the ability to resolve spatial structure with high resolutions of 2-20 μm at depths of 1-2 mm using backscattered near infrared light 2 – 5 . Fluorescence spectroscopy and fluorescence confocal microscopy systems provide information about biochemistry used for early cancer detection with depth weighted average fluorescence 6 – 9 .…”

Section: Introductionmentioning

confidence: 99%

Volume holographic reflection endoscope for in-vivo ovarian cancer clinical studies

et al. 2014

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We present the design for an endoscopic system capable of imaging tissues of the ovary at two selected imaging depths simultaneously. The method utilizes a multiplexed volume hologram to select wavefronts from different depths within the tissue. It is the first demonstration of an endoscopic volume holographic imaging system. The endoscope uses both gradient index (GRIN) optical components and off the shelf singlet lenses to relay an image from the distal tip to the proximal end. The endoscope has a minimum diameter of 3.75 mm. The system length is 30 cm which is connected to a handle that includes the holographic components and optics that relay the image to a camera. Preliminary evaluation of the endoscope was performed with tissue phantoms and calibrated targets, which shows lateral resolution ≈ 4 μm at an operating wavelength of 660 nm. The hologram is recorded in phenanthraquinone doped poly methacrylate and is designed to produce images from two tissue depths. One image is obtained at the tissue surface and the second 70 μm below the surface. This method requires no mechanical scanning and acquires an image at the camera frame rate. The preliminary ex-vivo results show good correlation with histology sections of the same tissue sections.

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Fluorescence-based surface magnifying chromoendoscopy and optical coherence tomography endoscope

Cited by 18 publications

References 22 publications

Dual optical modality endoscopic imaging of cancer development in the mouse colon

Dual optical modality endoscopic imaging of cancer development in the mouse colon

Ultraminiature optical design for multispectral fluorescence imaging endoscopes

Volume holographic reflection endoscope for in-vivo ovarian cancer clinical studies

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