Volume holographic reflection endoscope for in-vivo ovarian cancer clinical studies

Howlett, Isela D.; Gordon, Michael; Brownlee, John; Barton, Jennifer K.; Kostuk, Raymond K.

doi:10.1117/12.2037859

Cited by 6 publications

(4 citation statements)

References 9 publications

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“…After passing through the volume hologram, the diffracted light is collected by a lens and imaged by an FPA. VHI has been implemented in applications such as endoscopy [137, 138] and microscopy [139, 140]. Despite the snapshot advantage, the number of depth layers that can be simultaneously imaged by VHI is extremely restricted (≤5) [133].…”

Section: Snapshot Multidimensional Imaging Implementations and Appmentioning

confidence: 99%

A review of snapshot multidimensional optical imaging: Measuring photon tags in parallel

2016

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Multidimensional optical imaging has seen remarkable growth in the past decade. Rather than measuring only the two-dimensional spatial distribution of light, as in conventional photography, multidimensional optical imaging captures light in up to nine dimensions, providing unprecedented information about incident photons’ spatial coordinates, emittance angles, wavelength, time, and polarization. Multidimensional optical imaging can be accomplished either by scanning or parallel acquisition. Compared with scanning-based imagers, parallel acquisition—also dubbed snapshot imaging—has a prominent advantage in maximizing optical throughput, particularly when measuring a datacube of high dimensions. Here, we first categorize snapshot multidimensional imagers based on their acquisition and image reconstruction strategies, then highlight the snapshot advantage in the context of optical throughput, and finally we discuss their state-of-the-art implementations and applications.

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Section: Snapshot Multidimensional Imaging Implementations and Appmentioning

confidence: 99%

A review of snapshot multidimensional optical imaging: Measuring photon tags in parallel

2016

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“…Optical techniques can often be miniaturized for endoscopy, are robust and are relatively inexpensive. Optical coherence tomography (OCT) 8 , fluorescence confocal imaging 9 , and volume holographic imaging 10,11 , among other techniques, have been implemented into laparoscopic endoscopes for in vivo imaging. Results have been promising, but laparoscopic procedures are too invasive to be considered a regularly minimally invasive screening or surveillance method.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally it needs to have a wide field of view in order to provide navigation and optical biopsy simultaneously. Fluorescence imaging is a promising candidate as it is capable of imaging a wide field of view and if used with UV or blue excitation wavelengths can be used to image autofluorescence of endogenous fluorophores [11][12][13] . Autofluorescence imaging is advantageous because it does not require any dyes or contrast agents that may not be safe or practical in vivo.…”

Section: Introductionmentioning

confidence: 99%

Multispectral fluorescence imaging of human ovarian and Fallopian tube tissue for early stage cancer detection

Tate

Baggett

Rice

et al. 2015

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIII

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With early detection, five year survival rates for ovarian cancer are over 90%, yet no effective early screening method exists. Emerging consensus suggests that perhaps over 50% of the most lethal form of the disease, high grade serous ovarian cancer, originates in the Fallopian tube. Cancer changes molecular concentrations of various endogenous fluorophores. Using specific excitation wavelengths and emissions bands on a Multispectral Fluorescence Imaging (MFI) system, spatial and spectral data over a wide field of view can be collected from endogenous fluorophores. Wavelength specific reflectance images provide additional information to normalize for tissue geometry and blood absorption. Ratiometric combination of the images may create high contrast between neighboring normal and abnormal tissue. Twenty-six women undergoing oophorectomy or debulking surgery consented the use of surgical discard tissue samples for MFI imaging. Forty-nine pieces of ovarian tissue and thirty-two pieces of Fallopian tube tissue were collected and imaged with excitation wavelengths between 280 nm and 550 nm. After imaging, each tissue sample was fixed, sectioned and H&E stained for pathological evaluation. Comparison of mean intensity values between normal, benign, and cancerous tissue demonstrate a general trend of increased fluorescence of benign tissue and decreased fluorescence of cancerous tissue when compared to normal tissue. The predictive capabilities of the mean intensity measurements are tested using multinomial logistic regression and quadratic discriminant analysis. Adaption of the system for in vivo Fallopian tube and ovary endoscopic imaging is possible and is briefly described.

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“…Standard wide-field endoscopy provides wide field-of-view (FoV) and three-dimensional (3D) information of tissue samples; however, wide-field endoscopy has poor optical sectioning ability to reject out-of-focus background, which limits its medical use for imaging thick biological samples [1,2]. Many recent improvements in optical fluorescence endoscopy have development of optical sectioning capabilities, particularly centered on increasing acquisition speed, and enhancing image quality of resolution and contrast [1,[3][4][5][6][7][8][9][10][11][12].…”

mentioning

confidence: 99%

Speckle illumination holographic non‐scanning fluorescence endoscopy

Lin

Singh

et al. 2018

Journal of Biophotonics

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Optical sectioning endoscopy such as confocal endoscopy offers capabilities to obtain three-dimensional (3D) information from various biological samples by discriminating between the desired in-focus signals and out-of-focus background. However, in general confocal images are formed through point-by-point scanning and the scanning time is proportional to the 3D space-bandwidth product. Recently, structured illumination endoscopy has been utilized for optically sectioned wide-field imaging, but it still needs axial scanning to acquire images from different depths of focal plane. Here, we report wide-field, multiplane, optical sectioning endoscopic imaging, incorporating 3D active speckle-based illumination and multiplexed volume holographic gratings, to simultaneously obtain images of fluorescently labeled tissue structures from different depths, without the need of scanning. We present the design, and implementation, as well as experimental data, demonstrating this endoscopic system's ability to obtain optically sectioned multiplane fluorescent images of tissue samples, with cellular level resolution in wide-field fashion, and no need for mechanical or optical axial scanning.(A) Schematic drawing of the SIHN endoscopy to simultaneously acquire multiplane images from different depths. (B) Uniform, and (C) SIHN illuminated images of standard fluorescence beads (25 μm in diameter) for the two axial planes. (D) Intensity profile on fluorescently labeled signal (ie, in-focus) and background (ie, out-of-focus) of microspheres.

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Volume holographic reflection endoscope for in-vivo ovarian cancer clinical studies

Cited by 6 publications

References 9 publications

A review of snapshot multidimensional optical imaging: Measuring photon tags in parallel

A review of snapshot multidimensional optical imaging: Measuring photon tags in parallel

Multispectral fluorescence imaging of human ovarian and Fallopian tube tissue for early stage cancer detection

Speckle illumination holographic non‐scanning fluorescence endoscopy

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