Fluctuation of Schistosome Circulating antigen Levels in Urine of Individuals with Schistosoma mansoni Infection in Burundi

Etten, Lisette van; Engels, Dirk; Krijger, F. W.; Nkulikyinka, L; Gryseels, B.; Deelder, André M.

doi:10.4269/ajtmh.1996.54.348

Cited by 30 publications

(22 citation statements)

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“…Sensitivity and specificity are similar to those reported for the serological detection of antigens (Delder et al 1989, Van Lieshout et al 1993. Low fluctuations of urinary elimination of these antigens and the number of eggs shed in the feces during seven collections in five days and three subsequent weeks have been observed (Van Etten et al 1996, Disch et al 1997.…”

Section: Diagnosis Per Antigen Detectionsupporting

confidence: 68%

Diagnosing Schistosomiasis

Rabello¹

1997

Mem. Inst. Oswaldo Cruz

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Section: Diagnosis Per Antigen Detectionsupporting

confidence: 68%

Diagnosing Schistosomiasis

Rabello¹

1997

Mem. Inst. Oswaldo Cruz

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“…The cozncentrations of circulating antigens show less day-to-day variation than do egg counts (De Jonge et al 1989a;Van Etten et al 1996) and can therefore be relied upon for diagnosis. Quanti®cation of circulating antigens is also a promising technique for dierentiation between past and newly acquired infections, as circulating antigens are cleared within a few weeks of successful therapy (Barsoum et al 1991; Van't Wout et al 1992;Van Lieshout et al 1993).…”

Section: Introductionmentioning

confidence: 99%

A monoclonal antibody against Schistosoma haematobium soluble egg antigen: efficacy for diagnosis and monitoring of cure of S. haematobium infection

Salah

Demerdash

Shaker

et al. 2000

Parasitology Research

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A monoclonal antibody (mAb), 2F/11F, raised against Schistosoma haematobium soluble egg antigen (SEA) was found to be nonreactive with S. mansoni SEA or other parasite antigens (Fasciola hepatica, Echinococcus granulosus). This IgG1 mAb recognized a repetitive epitope on S. haematobium SEA in the molecular-weight regions of 70, 42, and 35 kDa. It was employed as both an antigen-capture and a biotinylated detection antibody in a sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of circulating schistosome antigen (CSA) and had a detection limit of <1 ng S. haematobium SEA/ml. CSA levels were measured in serum and urine samples from 116 S. haematobium-infected rural students before therapy and at 4, 8, and 12 weeks after praziquantel treatment. Serum and urine samples from 50 S. mansoni -infected patients, 15 patients harboring other parasites, and 30 noninfected individuals were also assessed. CSA was detected in 90.5% of serum samples and 94% of urine samples from S. haematobium-infected patients. CSA was undetectable in serum from the 15 patients harboring other parasites and in 94% of serum samples and 84% of urine samples from S. mansoni-infected patients. In the S. haematobium-infected group a positive correlation was detected between CSA levels in serum and urine samples and the egg load per 10 ml urine. A significant reduction in CSA levels was detected in serum and urine samples after praziquantel therapy. CSA was undetectable in 87% of serum samples and 81.5% of urine samples from schistosomiasis haematobium patients at 12 weeks post-treatment. These data demonstrate that the use of mAb 2F/11F for detection of CSA provides a sensitive method for the immunodiagnosis and monitoring of cure of schistosomiasis haematobium.

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“…In some studies, the consumption of fluid and involvement in physical exercise by the patients before urine samples are collected and examined have been shown to stabilize the egg output. However, in large studies especially within the community, using this approach would cause a logistic problem (Van Etten et al 1996;Kahama et al 1999). Several investigators reported a significant correlation between SEA levels and egg counts, hematuria, and ultrasound-detectable pathology Demerdash et al 1995;Shaker et al 1998;Kahama et al 1998;Salah et al 2000).…”

Section: Discussionmentioning

confidence: 97%

Human schistosomiasis haematobium: effective diagnosis of active infection using a pair of monoclonal antibodies against soluble egg antigen

et al. 2006

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The present study was designed to prepare monoclonal antibodies (MAbs) against Schistosoma haematobium soluble egg antigen (SEA) with immunodiagnostic potential for urinary schistosomiasis. From a panel of MAbs, a pair of IgG1 MAbs (2D/11C and 10B/2C) specific for S. haematobium SEA was selected. Both MAbs recognized one band with a 42-kDa molecular weight by western blots. The pair of MAbs was employed in sandwich ELISA for the detection of circulating schistosome antigen (CSA), one as antigen-capturing antibody and the other as peroxidase-conjugated antigen-detecting antibody. The lower detection limit of the assay was 1 ng/ml of S. haematobium SEA. The assay was performed on sera of 65 S. haematobium-infected patients, 25 patients infected with other parasites (Fasciola hepatica, Echinococcus granulosus), and 20 noninfected individuals. CSA was demonstrated in 89% of the S. haematobium-infected group. However, CSA was negative in the sera of healthy individuals and patients infected with other parasites, giving an overall specificity of 100% for the CSA assay. A positive correlation (r=0.37, p<0.01) was detected between the number of S. haematobium eggs excreted in 10 ml urine and the CSA level detected in the sera of S. haematobium-infected patients. Our data show that the use of anti-S. haematobium MAbs for the detection of CSA provides a sensitive and specific method for the immunodiagnosis of active S. haematobium-infected patients. Moreover, CSA assay using this anti-S. haematobium MAb/ELISA system was proven to correlate with intensity of infection and hence morbidity assessment.

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Fluctuation of Schistosome Circulating antigen Levels in Urine of Individuals with Schistosoma mansoni Infection in Burundi

Cited by 30 publications

References 0 publications

Diagnosing Schistosomiasis

Diagnosing Schistosomiasis

A monoclonal antibody against Schistosoma haematobium soluble egg antigen: efficacy for diagnosis and monitoring of cure of S. haematobium infection

Human schistosomiasis haematobium: effective diagnosis of active infection using a pair of monoclonal antibodies against soluble egg antigen

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