Human schistosomiasis haematobium: effective diagnosis of active infection using a pair of monoclonal antibodies against soluble egg antigen

Salah, Faten; Bassiouny, A. E. El; Rabia, Ibrahim; Demerdash, Zeinab; Roshdy, Mamdouh; Shaker, Z

doi:10.1007/s00436-005-0016-8

Cited by 13 publications

(5 citation statements)

References 32 publications

(53 reference statements)

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“…Similarly, Salah et al (2006) detected SEA in serum samples of S. haematobium infected patients. They reported that the procedure gave a sensitivity of 89% and a speci icity of 100% .also, in agreement with the current study, Elkawaz and Ghaffarifar (2009) detected adult worm antigen in S. haematobium infected serum by use ELISA and showed a sensitivity of 82% and speci icity of 95%.…”

Section: Discussionmentioning

confidence: 82%

Effective diagnosis of schistosomiasis haematobium by Silver beads Sandwich ELISA

Aly¹,

Zaahkouk²,

Samn³

et al. 2020

ijrps

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Schistosomiasis is a major public health problem. Diagnosis by simple and rapid immunoassays is a priority. The magnetic bead immunoassay using magnetic nanoparticles conjugated with anti-schistosomal antibody was evaluated for diagnosing human schistosomiasis infection. The present study was to evaluate the sandwich ELISA as a simple test for the detection of schistosomal antigen (CSA) in serum and urine samples of S. haematobium patients and compare it with ELISA. Investigation conducted on eighty six cases divided to three groups, 34 were positively for Schistosoma haematobium, 32 were positively for intestinal parasites ova and negative for S. haematobium ova in urine and 20 were negative urine and stool examination (Control). Immunomagnetical bead based Enzyme-linked immunosorbent assay (ELISA) using for detected for antigen in sera and urine infected by S. haematobium. Sandwich ELISA sensitivities was 79.4% (serum) and 73.5% (urine) and which increase by used nano-sandwich ELISA to 88.2% (serum) and 82.4% (urine), respectively. Sandwich ELISA specificities was 86.4% (serum) and 80.8% (urine) and which increase by used nano-sandwich ELISA to 93.3% (serum) and 88.5% (urine). We found that, nano-sandwich ELISA assay had highly sensitive and specifically and technical method was applicably, fast, cheaper, accurate and promising diagnostic method for schistosomiasis.

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Section: Discussionmentioning

confidence: 82%

Effective diagnosis of schistosomiasis haematobium by Silver beads Sandwich ELISA

Aly¹,

Zaahkouk²,

Samn³

et al. 2020

ijrps

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“…Serological assays can detect antibodies against schistosomal antigens in the blood, and are useful especially for the diagnosis for symptomatic travelers [1]. However, it is difficult to discriminate between the active infection and past exposure in people in endemic areas [1], and cross-reaction with other parasites can occur [13]. PCR techniques have also been introduced for diagnosis [14] and DNA detection for serum or urine was developed and being evaluated [15,16].…”

Section: Discussionmentioning

confidence: 99%

Case Report of Urinary Schistosomiasis in a Returned Traveler in Korea

et al. 2020

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A 23-year-old Korean woman with a residence history in Kenya and Malawi for about 2 years presented with gross hematuria for 1 month. Blood tests were within normal range except eosinophilia. Asymmetrically diffuse wall thickening and calcification were observed at the urinary bladder on CT. Multiple erythematous nodular lesions were observed in the cystoscopy and transurethral resection was done. Numerous eggs of Schistosoma haematobium with granulomatous inflammation were observed in the submucosal layer of the bladder. The patient was diagnosed with schistosomiasis-related cystitis and treated with praziquantel (40 mg/kg/day) twice before and after transurethral resection. This case suggests that S. haematobium infection should be considered as a cause of hematuria in Korea when the patient had a history of traveling endemic areas of schistosomiasis.

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“…The use of MAbs in CSA assays has greatly improved their specificity and sensitivity. 18,19,20,21 The need of serum-free media for the production of MAbs by hybridoma cells has been clearly established by many authors. 2,7,8 There are many important problems associated with serum-containing media, the most important being the high protein load, which makes product purification more difficult, the high cost serum and the risk of contamination.…”

Section: Discussionmentioning

confidence: 99%

Serum low replacement medium versus serum rich replacement medium for production of Anti-Schistosoma Monoclonal antibody: A comparative study

Mahmoud

Demerdash

Elmotawakel

et al. 2020

Clinical Epidemiology and Global Health

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Objective: to assess the production of Anti-Schistosoma Monoclonal Antibodies by growing IgM producing hybridoma cell line in serum low medium and to evaluate their diagnostic potential to replace the old conventional method in animal ascetic fluid. Methods: Highly reactive and specific IgM MAb secreting hybridoma cell line to S. mansoni SEA was cultured in three different media; serum rich replacement medium (conventional), serum low replacement medium (SLRM) and serum free replacement medium (SFRM). Results: Cell count and viability were better in conventional medium and SLRM than SFRM. The lower detection limit of the assay Mab of SLRM culture was 1.1 ng/ml of S. mansoni SEA. SFRM was excluded because of the low concentration and reactivity. MAb was tested against 34 human samples. Sensitivity and specificity of the assay for each were 92% and specificity 90% for SLRM respectively. The diagnostic efficacy of the assay was 94%. Conclusions: the production and diagnostic efficacy of antischistosomal MAbs was comparable on usage of either in vitro cell culture supernate in SLRM or conventional media.

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Human schistosomiasis haematobium: effective diagnosis of active infection using a pair of monoclonal antibodies against soluble egg antigen

Cited by 13 publications

References 32 publications

Effective diagnosis of schistosomiasis haematobium by Silver beads Sandwich ELISA

Effective diagnosis of schistosomiasis haematobium by Silver beads Sandwich ELISA

Case Report of Urinary Schistosomiasis in a Returned Traveler in Korea

Serum low replacement medium versus serum rich replacement medium for production of Anti-Schistosoma Monoclonal antibody: A comparative study

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