Abstract:Objective: to assess the production of Anti-Schistosoma Monoclonal Antibodies by growing IgM producing hybridoma cell line in serum low medium and to evaluate their diagnostic potential to replace the old conventional method in animal ascetic fluid. Methods: Highly reactive and specific IgM MAb secreting hybridoma cell line to S. mansoni SEA was cultured in three different media; serum rich replacement medium (conventional), serum low replacement medium (SLRM) and serum free replacement medium (SFRM). Results:… Show more
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