Flow-Injection Chemiluminescence Determination of Ofloxacin and Levofloxacin in Pharmaceutical Preparations and Biological Fluids

Sun, Hanwen; Li, Liqing; Chen, Xueyan

doi:10.2116/analsci.22.1145

Cited by 27 publications

(18 citation statements)

References 14 publications

(16 reference statements)

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“…8 The quantification of drugs or drug monitoring based on DNA-drug interactions has been reported. [9][10][11][12] DNA is usually chosen as a surface-modification element because of its high sensitivity, and compatibility with modern micro-fabrication technologies; it is especially promising for rapid detection. 13,14 Measurements of some trace phenothiazine drugs were performed with a DNA-modified carbon paste electrode (CPE) by using a potentiometric stripping analysis (PSA) method.…”

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confidence: 99%

Electrochemical Determination of Trace Promethazine Hydrochloride by a Pretreated Glassy Carbon Electrode Modified with DNA

et al. 2007

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Phenothiazines are a group of basic drugs that include a phenothiazine ring with different substituents attached at the 2-and 10-position, which are used as anti-psychotics, neuroleptics and antihistamines. 1 Several methods have been applied to detect phenothiazine derivatives, such as spectrophotometry, 2 colorimetry, 3 liquid chromatography (LC) 4 and titrimetry. 5 Promethazine hydrochloride, a prominent compound in the large group of phenothiazine derivatives, is widely used as a therapeutic agent for treating various mental and personality disorder. Daniela et al. 6 have reported the determination of promethazine hydrochloride in a pharmaceutical preparation using a flow-injection spectroelectroanalytical method. Lara et al. 7 utilized a capillary zone electrophoresis (CZE) technique for the quantitative analysis of three kinds of phenothiazines. The detection limit for promethazine hydrochloride was obtained to be 3.3 μg/mL; DPV and DC techniques were also applied to detect some phenothiazine drugs. 8 The quantification of drugs or drug monitoring based on DNA-drug interactions has been reported. [9][10][11][12] DNA is usually chosen as a surface-modification element because of its high sensitivity, and compatibility with modern micro-fabrication technologies; it is especially promising for rapid detection. 13,14 Measurements of some trace phenothiazine drugs were performed with a DNA-modified carbon paste electrode (CPE) by using a potentiometric stripping analysis (PSA) method. DNA-modified CPE permits measurements at low concentration levels of some phenothiazines with an accumulation step. 15 These previous studies suggested that a DNA-modified electrode might provide a very promising basis for determining trace promethazine.In the present work, DNA-modified electrode was assembled by immobilizing dsDNA onto the surface of a pretreated glassy carbon electrode (GCE(ox)). A pair of well-defined reversible redox peaks of promethazine was observed at low potential on the DNA/GCE(ox). The redox peak current was evaluated by using CV and DPV techniques. The signal was about 100-fold higher on DNA/GCE(ox) than that obtained on the DNA/GCE. By means of the redox peak currents of promethazine sensitively responding to the promethazine concentration, DNA/GCE(ox) could be applied to determine trace promethazine. The influence of a glassy carbon electrode pretreatment on the sensitivity of determining promethazine was considered. DNA/GCE(ox) was used to determine promethazine in healthy human serum with satisfactory results. Experimental Apparatus and reagentsElectrochemical measurements were performed with a CHI660A electrochemical analyzer (Shanghai Chenhua Apparatus Corporation, China). Glassy carbon electrodes (diameter 3 mm) and DNA modified glassy carbon electrodes were used as the working electrode, respectively. A platinum auxiliary electrode and an Ag/AgCl reference electrode were used for the measurements.Calf thymus (CT) DNA (Cat. No. D1501) and promethazine hydrochloride (Cat. No. P4651) were purchase...

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Electrochemical Determination of Trace Promethazine Hydrochloride by a Pretreated Glassy Carbon Electrode Modified with DNA

et al. 2007

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“…Unlike the other reported methods [12][13][14][15]19], the proposed methods are very simple, free from the use of auxiliary reagents and expensive instruments. The reported visible spectrophotometric methods based on oxidative-coupling reaction [23] uses an expensive reagent, and the reaction product is less stable.…”

Section: Discussionmentioning

confidence: 99%

“…HPLC has been used for the assay of OFX in pharmaceuticals when present either alone [5][6][7] or in combination with nitazoxanide [8]. Various other techniques including HPTLC [9], capillary electrophoresis [10,11], atomic absorption spectrometry [12], spectrofluorometry [12], chemiluminescence spectrometry [13][14][15], UV-spectrophotometry [16][17][18], stripping voltammetry [19], potentiometry and conductometry [20], polarography [21], and microbiological method [22] have been reported for the assay of OFX in pharmaceuticals. Many of the above techniques are deficient on simplicity, cost-effectiveness, and easy accessibility.…”

Section: Introductionmentioning

confidence: 99%

Simple and Selective Spectrophotometric Determination of Ofloxacin in Pharmaceutical Formulations Using Two Sulphonphthalein Acid Dyes

2013

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Two new simple and sensitive extraction-free spectrophotometric methods have been established for the determination of ofloxacin (OFX). The methods are based on ion-pair complex formation reaction between OFX and acidic sulphonphthalein dyes, bromocresol purple (method A), and bromocresol green (method B) in dichloromethane. The experimental variables such as reaction medium, reaction time, and reagent concentration have been carefully optimized to achieve the highest sensitivity. Both dyes react spontaneously with OFX to give yellow-colored complexes. Beer's law is obeyed over the concentration ranges of 1.0-16 g ml . The limits of detection (LOD) and quantification (LOQ) are also reported. A Job's plot of the absorbance versus the molar ratio of OFX to each of dyes under consideration indicated (1 : 1) ratio and the conditional stability constant ( ) of the complexes have been calculated. The proposed methods were applied successfully to the determination of OFX in tablets with good accuracy and precision and without interference from common additives. The results obtained by the proposed methods were compared favorably with those of the reference method.

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“…Monitoring OFL concentrations in real samples is vital for adjusting the drug dosage and studying drug-drug interactions. 3 H i g h -p e r f o r m a n c e l i q u i d c h r o m a t o g r a p hy (HPLC), [4][5][6] spectrophotometry, 7,8 spectrofluorimetry, 9,10 thin layer chromatography (TLC)-fluorescence spectrodensitometry, 11,12 capillary electrophoresis, 13,14 and flow-injection chemiluminescence 15,16 are several methods used for ofloxacin determination. Some of these methods are expensive, elaborate, and often not sensitive enough to directly determine trace amounts of ofloxacin in urine samples.…”

Section: Introductionmentioning

confidence: 99%

Application of Response Surface Modeling and Chemometrics Methods for the Determination of Ofloxacin in Human Urine Using Dispersive Liquid-Liquid Microextraction Combined with Spectrofluorimetry

Assadian

Ниази

2017

J. Braz. Chem. Soc.

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Dispersive liquid-liquid microextraction (DLLME) and spectrofluorimetry, coupled with chemometrics methods, are proposed in this work for the pre-concentration and determination of ofloxacin concentration in spiked human urine. Chloroform and acetonitrile were selected as the extraction and dispersive solvents by the one-variable-at-a-time process. The Box-Behnken design was used to optimize the other variables, including the volume of extraction and dispersion solvents, solution pH, and ionic strength. A linear calibration curve was obtained in the 5.0-120.0 ng mL -1 range under optimal conditions with a detection limit of 1.61 ng mL -1 and correlation coefficient of 0.9948. A relative standard deviation (RSD) of 1.13% was obtained for seven consecutive replicates. Parallel factor analysis (PARAFAC) and partial least square (PLS) modeling were applied for the multivariate calibration of the spectrofluorimetric data. To pre-process the data matrices and predict the model results, the orthogonal signal correction (OSC) was used, and the analysis results were statistically compared. The methods accuracy values for ofloxacin determination, evaluated by the root mean square errors of prediction (RMSEP) and relative standard error of prediction (RSEP), were 0.82 and 1.12 using OSC-PLS, and 0.31 and 0.42 using OSC-PARAFAC models, respectively. Ofloxacin can be reliably determined in human urine samples through the proposed procedure, according to the results.

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Flow-Injection Chemiluminescence Determination of Ofloxacin and Levofloxacin in Pharmaceutical Preparations and Biological Fluids

Cited by 27 publications

References 14 publications

Electrochemical Determination of Trace Promethazine Hydrochloride by a Pretreated Glassy Carbon Electrode Modified with DNA

Electrochemical Determination of Trace Promethazine Hydrochloride by a Pretreated Glassy Carbon Electrode Modified with DNA

Simple and Selective Spectrophotometric Determination of Ofloxacin in Pharmaceutical Formulations Using Two Sulphonphthalein Acid Dyes

Application of Response Surface Modeling and Chemometrics Methods for the Determination of Ofloxacin in Human Urine Using Dispersive Liquid-Liquid Microextraction Combined with Spectrofluorimetry

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