Simple and Selective Spectrophotometric Determination of Ofloxacin in Pharmaceutical Formulations Using Two Sulphonphthalein Acid Dyes

Abstract

Two new simple and sensitive extraction-free spectrophotometric methods have been established for the determination of ofloxacin (OFX). The methods are based on ion-pair complex formation reaction between OFX and acidic sulphonphthalein dyes, bromocresol purple (method A), and bromocresol green (method B) in dichloromethane. The experimental variables such as reaction medium, reaction time, and reagent concentration have been carefully optimized to achieve the highest sensitivity. Both dyes react spontaneously with OFX to give yellow-colored complexes. Beer's law is obeyed over the concentration ranges of 1.0-16 g ml . The limits of detection (LOD) and quantification (LOQ) are also reported. A Job's plot of the absorbance versus the molar ratio of OFX to each of dyes under consideration indicated (1 : 1) ratio and the conditional stability constant ( ) of the complexes have been calculated. The proposed methods were applied successfully to the determination of OFX in tablets with good accuracy and precision and without interference from common additives. The results obtained by the proposed methods were compared favorably with those of the reference method.

A joint action of aptamers and gold nanoparticles chemically trapped on a glassy carbon support for the electrochemical sensing of ofloxacin

“…Several studies were performed for the electrochemical detection of OFL (Table 3) [13][14][15][16][17][18][19]78]. Electrochemical biosensing of OFL was first performed by Zhang et al where an electrochemical immunosensor was developed for OFL detection by employing a polypyrrole film-Au nanoclusters modified GC electrode as a sensor platform and horseradish peroxidase/horseradish peroxidase secondary antibody immobilized on gold nanorods as the detection label [13].…”

“…However, it has been shown that 70% of OFL was excreted in unmetabolized form into water environment, leading to negative effects on human health [5]. As a result, many methods have been developed for the determination of OFL, including electrochemical methods [9][10][11][12][13][14][15][16], spectrophotometric methods [17,18], and chromatographic methods [19,20]. However, in spite of their high selectivity and sensitivities (LOD of 0.01-16 µg/ml (≈2.7×10 -8 -4.4×10 -5 M), these techniques are time-consuming, require complex sample procedures and involve the use of large amounts of reagents and organic solvents thus need considerable costs.…”

Determination of levofloxacin, norfloxacin, and moxifloxacin in pharmaceutical dosage form or individually using derivative UV spectrophotometry

“…The resulting MIL-100(Fe) effectively served as an adsorbent for the extraction of the antibiotic levofloxacin from the aqueous phase [20]. More research technique were reviewed [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37].…”

Biogenic synthesis of ZnO and Al2O3 nanoparticles using Camellia sinensis and Origanum vulgare L. leaves extract for spectroscopic estimation of ofloxacin and ciprofloxacin in commercial formulations

“…They are used to treat infections of the respiratory system, community-acquired pneumonia, urinary tract, bone, and soft tissue [ 6 , 7 ]. Chromatographic approaches [ 8 , 9 ], spectrophotometric methods [ 10 , 11 ], chemiluminescence methods [ 12 , 13 ], and electrochemical methods [ 14 , 15 ] have all been presented for determining OFX and CPFX in various analytical matrices.…”