Flavobacterium petrolei sp. nov., a novel psychrophilic, diesel-degrading bacterium isolated from oil-contaminated Arctic soil

Abstract

This study presents taxonomic description of two novel diesel-degrading, psychrophilic strains: Kopri-42T and Kopri-43, isolated during screening of oil-degrading psychrotrophs from oil-contaminated Arctic soil. A preliminary 16S rRNA gene sequence and phylogenetic tree analysis indicated that these Arctic strains belonged to the genus Flavobacterium, with the nearest relative being Flavobacterium psychrolimnae LMG 22018T (98.9% sequence similarity). The pairwise 16S rRNA gene sequence identity between strains Kopri-42T and Kopri-43 was 99.7%. The DNA-DNA hybridization value between strain Kopri-42T and Kopri-43 was 88.6 ± 2.1% indicating that Kopri-42T and Kopri-43 represents two strains of the same genomospecies. The average nucleotide identity and in silico DNA-DNA hybridization values between strain Kopri-42T and nearest relative F. psychrolimnae LMG 22018T were 92.4% and 47.9%, respectively. These values support the authenticity of the novel species and confirmed the strain Kopri-42T belonged to the genus Flavobacterium as a new member. The morphological, physiological, biochemical and chemotaxonomic data also distinguished strain Kopri-42T from its closest phylogenetic neighbors. Based on the polyphasic data, strains Kopri-42T and Kopri-43 represents a single novel species of the genus Flavobacterium, for which the name Flavobacterium petrolei sp. nov. is proposed. The type strain is Kopri-42T (=KEMB 9005-710T = KACC 19625T = NBRC 113374T).

Comparative Transcriptome and Endophytic Bacterial Community Analysis of Morchella conica SH

“…As observed, high abundances of Flavobacterium , Pseudomonas , and Pedobacter were found in the four stages, while Massilia and Herbaspirillum were highly abundant at the first three stages or mature stages, respectively ( Figure 5C ). As previously reported, Flavobacterium associated with promoting the development of its host ( Chaudhary et al, 2019 ; Kim and Yu, 2020 ). Massilia was involved in the synthesis of multiple secondary metabolites and enzymes, phosphorus solubilization, degradation of phenanthrene, and resistance to heavy metals ( Feng et al, 2016 ; Zheng et al, 2017 ).…”

Flavobacterium baculatum sp. nov., a carotenoid and flexirubin-type pigment producing species isolated from flooded paddy field

“…As of August 2020, the genus Flavobacterium consist of more than 287 species that have been retrieved from wide range of habitats including forest soil, farmed salmonid fish, primates' faeces, fresh water, waste water treatment plant and seawater [2][3][4][5][6][7][8]. Some species of the genus Flavobacterium are pathogenic to fish [4] and some have been found to degrade diesel [9]. Members of the genus Flavobacterium represent one of the most abundant bacterial genera prevalent in glaciers [10].…”

Flavobacterium muglaense sp. nov. isolated from internal organs of apparently healthy rainbow trout

“…Oxidase activity was determined by monitoring the oxidation of tetramethyl- p -phenylenediamine on filter paper. The antimicrobial susceptibility test was performed on R2A plates using the paper disc method with the following commercial antibiotics: neomycin (30 µg), gentamicin (10 µg), enrofloxacin (10 µg), danofloxacin (5 µg), erythromycin (15 µg), amoxicillin (25 µg), amoxicillin and clavulanic acid (30 µg), florfenicol (30 µg), sulfamethoxazole and trimethoprim (25 µg), oxolinic acid (2 µg), tetracycline (10 µg), oxytetracycline (30 µg), chlortetracycline (30 µg), doxycycline (30 µg), and vibriostatic agent O129 (10 and 150 µg) [32, 33]. Growth of the strains was assessed using the following bacteriological media: R2A agar (BD Difco), MacConkey agar (MC; Merck), nutrient agar (NA; Merck), sea water agar (Media), marine agar 2216 (BD Difco) and tryptic soy agar (TSA; Merck).…”

“…commercial antibiotics: neomycin (30 µg), gentamicin (10 µg), enrofloxacin (10 µg), danofloxacin (5 µg), erythromycin (15 µg), amoxicillin (25 µg), amoxicillin and clavulanic acid (30 µg), florfenicol (30 µg), sulfamethoxazole and trimethoprim (25 µg), oxolinic acid (2 µg), tetracycline (10 µg), oxytetracycline (30 µg), chlortetracycline (30 µg), doxycycline (30 µg), and vibriostatic agent O129 (10 and 150 µg)[32,33].…”