Flavisolibacter ginsengiterrae gen. nov., sp. nov. and Flavisolibacter ginsengisoli sp. nov., isolated from ginseng cultivating soil

Abstract

Two strains (Gsoil 492T and Gsoil 643T) isolated in Pocheon Province, South Korea, from soil used for ginseng cultivation were characterized using a polyphasic approach. Both isolates comprised Gram-negative, aerobic, non-motile, rod-shaped bacteria. They had similar chemotaxonomic characteristics, e.g. containing MK-7 as the major quinone, having a DNA G+C content in the range 42.5–43.3 mol% and possessing iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. A phylogenetic analysis based on 16S rRNA gene sequences indicated that the two isolates formed a tight cluster with several uncultured bacterial clones and with the established genera Terrimonas, Niastella and Chitinophaga in the phylum Bacteroidetes but were clearly separate from these genera. The levels of 16S rRNA gene sequence similarity between the isolates and type strains of related genera ranged from 87.5 to 92.4 %. Furthermore, the results of physiological and biochemical tests allowed phenotypic differentiation of the isolates from phylogenetically closely related species with validly published names. The level of 16S rRNA gene sequence similarity between the two strains was 99.5 %, whereas the DNA–DNA relatedness value was 44 %, indicating that they represent separate species. On the basis of the polyphasic evidence, a novel genus, Flavisolibacter gen. nov., and two novel species, Flavisolibacter ginsengiterrae sp. nov. (type strain Gsoil 492T=KCTC 12656T=DSM 18136T) and Flavisolibacter ginsengisoli sp. nov. (type strain Gsoil 643T=KCTC 12657T=DSM 18119T), are proposed. Flavisolibacter ginsengiterrae is the type species of the genus.

Bacterial Diversity in Bentonites, Engineered Barrier for Deep Geological Disposal of Radioactive Wastes

“…Clones belonging to family Chitinophagaceae were also identified: BI-2-2 affiliated to Flavisolibacter ginsengisoli strain Gsoil 643 [53], and BI-2-64 affiliated to Flavisolibacter sp. MDT2-37 (Online Interestingly, one clone sequence belonged to phylum Deinococcus-Thermus, which was only found in sample BI-2.…”

Hydrotalea flava gen. nov., sp. nov., a new member of the phylum Bacteroidetes and allocation of the genera Chitinophaga, Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas and Hydrotalea to the family Chitinophagaceae fam. nov.

“…The most closely related species was found to be Sediminibacterium salmoneum (Qu & Yuan, 2008) with a 16S rRNA gene sequence similarity of 92.5 % to the three strains. Lower 16S rRNA gene sequence similarites were found with representatives of the genera Chitinophaga (Sangkhobol & Skerman, 1981), Terrimonas (Xie & Yokota, 2006), Niastella (Weon et al, 2006), Niabella (Kim et al, 2007), Flavisolibacter (Yoon & Im, 2007), Segetibacter , Parasegetibacter (Zhang et al, 2009), Lacibacter (Qu et al, 2009), Ferruginibacter (Lim et al, 2009), Filimonas (Shiratori et al, 2009) and Flavihumibacter (Zhang et al, 2010). All of these genera formed a distinct cluster in the 16S rRNA gene sequence-based tree and showed some phenotypic similarities.…”

“…On NA, colonies are orange-yellow and circular with an entire margin. DCells were grown for 3 days on one-tenth-strength trypticase soy agar (TSA) (temperature not specified) (Yoon & Im, 2007). dCells were grown for 3 days at 29 u C on TSA (Xie & Yokota, 2006).…”

Niastella populi sp. nov., isolated from soil of Euphrates poplar (Populus euphratica) forest, and emended description of the genus Niastella

“…In terms of physiological and biochemical properties, strain THYL-44 T could be distinguished from members of the genera Terrimonas (Xie & Yokota, 2006), Flavisolibacter and Segetibacter (Yoon & Im, 2007) by the ability to hydrolyse casein, the absence of oxidase activity and the enzyme reaction pattern in the API ZYM test kit. Furthermore, strain THYL-44 T could be differentiated from the two recognized members of the genus Niastella on the basis of its ability to assimilate a number of carbohydrates, inability to hydrolyse chitin and absence of gliding motility.…”