FISH IMMUNOLOGY. I. BINDING AND ENGULFMENT OFCANDIDA ALBICANSBY ERYTHROCYTES OF RAINBOW TROUT (SALMO GAIRDNERIRICHARDSON)

Abstract

The role of fish erythrocytes (FE) as phagocytic cells has poorly been investigated, until now. Here, we have focussed our attention on the interplay between rainbow trout (Salmo gairdneri Richardson) erythrocytes and Candida albicans (CA). At the same time, the intervention of autologous head kidney macrophages (MO) in the CA processing by FE has been studied. Data show that CA particles bind to FE, which, in turn, are able to engulf but not kill them. In the presence of MO, a decrease of FE with bound CA occurs and, in some microscopic images, FE form rosettes with MO. Phagocytosis of CA is higher in rosetting MO than in non-rosetting ones. According to our findings, it appears that FE represent a reservoir of engulfed CA and rosetting is an efficacious phenomenon of presentation of pathogens to MO, where an effective clearance of them can take place.

Dietary vitamin A requirement of juvenile Wuchang bream ( Megalobrama amblycephala ) determined by growth and disease resistance

“…Erythrocytes can induce blood B cells to produce immunoglobulin through proliferation, differentiation and maturity (Boackle et al, 1998) and has been shown to actively form rosettes to facilitate the clearance of pathogens by macrophages (Passantino et al, 2002), while leucocytes can migrate in response to inflammatory stimuli (Carroll, 2004). Hemoglobin is an important source of bioactive peptides that have been shown to participate in the innate immune response and participate in the killing of invading microbes (Bishlawy, 1999;Jiang et al, 2007).…”

Immunotoxicity and hepatic function evaluation in Nile tilapia (Oreochromis niloticus) exposed to diazinon

“…Exposure of fish to diazinon polluted water caused pathological changes in fish and activated the monocytesemacrophage [28]. The innate immune response of Nile tilapia to these OPs was evaluated through the determination of two phagocytic parameters: PI and %AC.…”

Route of entry and tissue distribution of Yersinia ruckeri in experimentally infected rainbow trout Oncorhynchus mykiss

“…Within melanomacrophage centres, macrophages have been shown to destroy and kill the fungus Candida albicans but also to phagocytose rainbow trout erythrocytes in the immediate vicinity (Passantino et al 2002). Consequently, melanomacrophage centers seem to play an important role in the elimination of foreign particulate material and of fish erythrocytes that have ingested microorganisms.…”