First description of milky disease in the Chinese mitten crab Eriocheir sinensis caused by the yeast Metschnikowia bicuspidata

Bao, Jie; Jiang, Hongbo; Shen, Hongbin; Xing, Yuenan; Feng, Chengcheng; Li, Xiaodong; Chen, Qijun

doi:10.1016/j.aquaculture.2020.735984

Cited by 33 publications

(60 citation statements)

References 18 publications

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“…In this study, we isolated and identified the pathogen causing the outbreak of the disease and submitted the first 18S rDNA and ITS gene sequences (MN551840/MN886520) of M. bicuspidata to GenBank in China. Our results were consistent with those reported by Bao et al (2021). Besides, our research also demonstrated a typical multilateral budding model of the yeast clearly.…”

supporting

confidence: 93%

“…Unlike the methodology used by Bao et al (2021), we adopted immersion infection while using injection infection. In addition, we also calculated the isolation rate and mortality, which were…”

Section: Discussionmentioning

confidence: 99%

“…ITS and 26S rDNA sequence analyses were performed followingZhang et al (2014), Clemente-Jimenezet al (2004 andBao et al (2021) with some modification. The primer names and sequences are listed in Table1.…”

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confidence: 99%

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Metschnikowia bicuspidata isolated from milky diseased Eriocheir sinensis: Phenotypic and genetic characterization, antifungal susceptibility and challenge models

JianNan

et al. 2021

Journal of Fish Diseases

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Eriocheir sinensis is one of the most important freshwater aquaculture species in China. In 2018, the total production of E. sinensis reached 692,723 tonnes in China, with a value of 9,386,536 thousand dollars (http://www.fao.org/fishe ry/stati stics ，2018). As the development of E. sinensis aquaculture continues, the occurrence of diseases is increasing. Currently, the diseases affecting E. sinensis include tremor disease caused by Spiroplasma infection (Wang et al., 2004), vibriosis caused by Vibrio anguillarum (Sui et al., 2012) and V. parahaemolyticus (Wagley et al., 2009), and parasitosis caused by a halacarid mite (Normant et al., 2013). In 2007, an emulsification disease characterized by milky liquid accumulation was reported in the crab Portunus trituberculatus and was attributed to Pseudomonas

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confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Metschnikowia bicuspidata isolated from milky diseased Eriocheir sinensis: Phenotypic and genetic characterization, antifungal susceptibility and challenge models

JianNan

et al. 2021

Journal of Fish Diseases

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“…Eriocheir sinensis (20 ± 3 g, 1: 1 female: male ratio) were purchased from a market in Panjin city, China, transported back to Shenyang Agricultural University, and temporarily cultured in 300 L tanks. Healthy E. sinensis [verified by M. bicuspidata negative results using PCR D1/D2 domain of the 26S rDNA sequence analyses ( 7 )] were cultured for 7 d prior to the experimental tests. The control group (n = 30) was injected with 100 μL of phosphate-buffered saline (pH 7.4), and the experimental group (n = 30) was injected with 100 μL of M. bicuspidata (10 7 cells/mL).…”

Section: Methodsmentioning

confidence: 99%

“…In 2018, some diseased E. sinensis with a milky-like liquid accumulated in vivo were found in Panjin city in the Liaoning province, and this condition was referred to as “milky disease.” After infection, the activity of infected crabs was weakened, their pereiopods easily fell off, and the crabs died. The infection rate was greater than 20% ( 7 ). The pathogen was identified as Metschnikowia bicuspidata .…”

Section: Introductionmentioning

confidence: 97%

Proteomic Analysis of the Hemolymph After Metschnikowia bicuspidata Infection in the Chinese Mitten Crab Eriocheir sinensis

et al. 2021

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The “milky disease” of the Chinese mitten crab, Eriocheir sinensis, is a highly lethal fungal disease caused by Metschnikowia bicuspidata infection. To elucidate the immune responses of the hemolymph of E. sinensis to M. bicuspidata infection, a comparative analysis of the hemolymph of E. sinensis infected with M. bicuspidata and that treated with phosphate buffered saline was performed using label-free quantitative proteomics. A total of 429 proteins were identified. Using a 1.5-fold change in expression as a physiologically significant benchmark, 62 differentially expressed proteins were identified, of which 38 were significantly upregulated and 24 were significantly downregulated. The upregulated proteins mainly included cytoskeleton-related proteins (myosin regulatory light chain 2, myosin light chain alkali, tubulin α-2 chain, and tubulin β-1 chain), serine protease and serine protease inhibitor (clip domain-containing serine protease, leukocyte elastase inhibitor, serine protein inhibitor 42Dd), catalase, transferrin, and heat shock protein 70. Upregulation of these proteins indicated that phenoloxidase system, phagocytosis and the ROS systems were induced by M. bicuspidata. The downregulated proteins were mainly organ and tissue regeneration proteins (PDGF/VEGF-related factor protein, integrin-linked protein kinase homing pat-4 gene) and hemagglutination-associated proteins (hemolymph clottable protein, hemocyte protein-glutamine gamma-glutamyltransferase). Downregulation of these proteins indicated that M. bicuspidata inhibited hemocyte regeneration and hemolymph agglutination. Fifteen differentially expressed proteins related to immunity were verified using a parallel reaction monitoring method. The expression trend of these proteins was similar to that of the proteome. To the best of our knowledge, this is the first report on the proteome of E. sinensis in response to M. bicuspidata infection. These results not only provide new and important information on the immune response of crustaceans to yeast infection but also provide a basis for further understanding the molecular mechanism of complex host pathogen interactions between crustaceans and fungi.

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Screening and identification of lactic acid bacteria with antimicrobial abilities for aquaculture pathogens in vitro

et al. 2022

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First description of milky disease in the Chinese mitten crab Eriocheir sinensis caused by the yeast Metschnikowia bicuspidata

Cited by 33 publications

References 18 publications

Metschnikowia bicuspidata isolated from milky diseased Eriocheir sinensis: Phenotypic and genetic characterization, antifungal susceptibility and challenge models

Metschnikowia bicuspidata isolated from milky diseased Eriocheir sinensis: Phenotypic and genetic characterization, antifungal susceptibility and challenge models

Proteomic Analysis of the Hemolymph After Metschnikowia bicuspidata Infection in the Chinese Mitten Crab Eriocheir sinensis

Screening and identification of lactic acid bacteria with antimicrobial abilities for aquaculture pathogens in vitro

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