Screening and identification of lactic acid bacteria with antimicrobial abilities for aquaculture pathogens in vitro

Zhang, Fengfeng; Zhou, Ke; Xie, Fucun; Zhao, Qiong

doi:10.1007/s00203-022-03285-y

Cited by 7 publications

(3 citation statements)

References 66 publications

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Section: Methodsmentioning

confidence: 99%

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The Effect of Lactobacillus plantarum on the Fecal Microbiota, Short Chain Fatty Acids, Odorous Substances, and Blood Biochemical Indices of Cats

Han,

Liang,

Sun

et al. 2024

Microorganisms

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Lactobacilli have played an important role in the gut health of pets. The aim of this research was to study the effects of isolated Lactobacilli (named L11) on the immune, nutrient metabolism, and gut health of cats. Twelve healthy adult cats were randomly assigned into two groups, the control group (CONTROL, n = 6, without any probiotics product) and the treatment group (probiotics, n = 6, L11 109 CFU/kg feed), while using the same dry diet. On day 28, blood and fecal samples were collected, and the blood biochemical indices, fecal microbiota, short-chain fatty acids (SCFAs), immunological parameters, and odorous substances were separately tested. The triglyceride of the blood was decreased after using L11 (p < 0.05), which could probably alleviate the occurrence of cat obesity to some extent. The sIgA of the feces was increased by 30.1% (p < 0.05), which could enhance the cat’s immunity. The abundance of Bifidobacteria was increased after using L11 (p < 0.05), and the indole and 3-methylindole of the feces were both reduced compared with the control group; 3-methylindole was especially reduced by 67.3% (p < 0.05), which showed that L11 could also improve the intestinal state of cats. Therefore, this research shows that L11 could be a good choice to improve the gut health and immune functions of cats, and it is probably related to the lipid mechanism of cats.

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Section: Methodsmentioning

confidence: 99%

“…The agar diffusion method was first used to test the inhibition on pathogens of the isolate [ 13 ]. First, the inhibitory effect of L11 on pathogens was tested by the agar diffusion method [ 13 ]. E. coli CVCC 1555, E. coli ATCC 14028, S. aureus ATCC 43300, and S. enteritidis CVCC 3377 were selected as indicators.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Lactobacillus plantarum on the Fecal Microbiota, Short Chain Fatty Acids, Odorous Substances, and Blood Biochemical Indices of Cats

Han,

Liang,

Sun

et al. 2024

Microorganisms

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“…The Oxford cup diffusion method was utilized to evaluate the comparative inhibitory effects of the encapsulated antibacterial peptides, following the procedure described by [ 27 ]. The bacterial broth containing three activated strains ( E. coli ATCC 8099, S. aureus ATCC 25923, and P. aeruginosa ATCC 27853) was centrifuged at 4000 rpm for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Assessing the Efficacy of PLGA-Loaded Antimicrobial Peptide OH-CATH30 Microspheres for the Treatment of Bacterial Keratitis: A Promising Approach

et al. 2023

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Bacterial keratitis in animals presents challenges due to ocular structural barriers, hindering effective drug delivery. In this study, we used biocompatible and biodegradable poly(lactic-co-glycolic acid) (PLGA) to encapsulate the naturally occurring antimicrobial peptide OH-CATH30, an alternative to conventional antibiotics, for the treatment of bacterial keratitis in animals. Microspheres (MS) were prepared using a modified water-in-oil-in-water (W/O/W) double-emulsion method with optimized osmotic pressure. We conducted comprehensive evaluations, including in vitro characterization, encapsulation efficiency determination, in vitro release kinetics, and in vivo/vitro assessments of irritation and bacterial inhibition. The optimized method yielded microspheres with impressive encapsulation efficiency of 75.2 ± 3.62% and a loading capacity of 18.25 ± 5.73%, exhibiting a well-defined particle size distribution (200–1000 nm) and a ζ-potential of −17.3 ± 1.91 mV. The microspheres demonstrated initial burst release followed by sustained and controlled release in vitro. Both in vitro and in vivo tolerance tests confirmed the biocompatibility of the drug-loaded microspheres, as they did not elicit significant irritation in ocular tissues. Remarkable antibacterial effects were observed in both in vitro and in vivo experiments. Our developed PLGA microspheres show promise as an alternative therapeutic option for topical administration in managing keratitis, offering exceptional drug delivery capabilities, improved bioavailability, and potent antibacterial efficacy.

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Co-production of surfactin and fengycin by Bacillus subtilis BBW1542 isolated from marine sediment: a promising biocontrol agent against foodborne pathogens

Yan,

Li,

Liang

et al. 2023

J Food Sci Technol

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Screening and identification of lactic acid bacteria with antimicrobial abilities for aquaculture pathogens in vitro

Cited by 7 publications

References 66 publications

The Effect of Lactobacillus plantarum on the Fecal Microbiota, Short Chain Fatty Acids, Odorous Substances, and Blood Biochemical Indices of Cats

The Effect of Lactobacillus plantarum on the Fecal Microbiota, Short Chain Fatty Acids, Odorous Substances, and Blood Biochemical Indices of Cats

Assessing the Efficacy of PLGA-Loaded Antimicrobial Peptide OH-CATH30 Microspheres for the Treatment of Bacterial Keratitis: A Promising Approach

Co-production of surfactin and fengycin by Bacillus subtilis BBW1542 isolated from marine sediment: a promising biocontrol agent against foodborne pathogens

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