Finger-Based Printed Sensors Integrated on a Glove for On-Site Screening Of Pseudomonas aeruginosa Virulence Factors

Abstract

Early screening of clinically relevant pathogens in the environment is a highly desirable goal in clinical care, providing precious information that will improve patient-care outcomes. In this work, a glove-based electrochemical sensor has been designed for point-of-use screening of Pseudomonas aeruginosa's virulence factors. The methodology used for the elaboration of the fabric platform relied on printing the conductive inks on the index and middle fingers of the glove, with the goal of screening pyocyanin and pyoverdine targets. The analytical signatures of the analytes were recorded in about 4 min, via the rapid and selective square-wave-voltammetry technique. Finger-based sensors display good performance and discrimination between the targets and potential interferences, along with good reproducibility. The sensors featured linearity over the 0.01-0.1 μM range for pyocyanin and 5-50 μM range for pyoverdine, with sensitivities of 2.51 μA/μM for pyocyanin and 1.09 nA/μM for pyoverdine ( R = 0.990 and 0.995, respectively) and detection limits of 3.33 nM for pyocyanin and 1.66 μM for pyoverdine. Moreover, the sensors were tested in binary mixtures of analytes, with successful outcomes. In order to gain information from the surrounding environment, the active electronic areas of the printed fingers were coated with a conductive hydrogel matrix, and relevant target surfaces were "swiped for notification" of contaminants. The simple fabrication, low-cost, and reusability of the proposed glove are likely to underpin the progressive drive of wearable sensors toward decentralized environmental and healthcare applications.

Toward a Closed Loop, Integrated Biocompatible Biopolymer Wound Dressing Patch for Detection and Prevention of Chronic Wound Infections

“…This shows that the results reported in our study with the biocompatible polymer are comparable to other work. Interestingly, Ciui et al (2018) produced a “swipeable” sensor, capable of detecting pyocyanin between 10 and 100 nM with a carbon electrode screen printed onto a disposable glove. This demonstrates that much higher sensitivities can be achieved with low cost, carbon-based substrates.…”

Rapid and highly sensitive detection of pyocyanin biomarker in differentPseudomonas aeruginosainfections using gold nanoparticles modified sensor

“…The results in Figure 5 showed the high sensitivity for detection of pyocyanin using the Au modified ITO, which attributed to the use of PANI/Au NPs modified ITO as a working electrode that achieved the precise, rapid and sensitive measurement of pyocyanin with low cost. Table 1 showed the LOD of our modified electrode in comparison with the LOD of the previously reported of some other electrodes for the electrochemical determination of pyocyanin that revealed that our electrode possessed lower LOD in comparison with most of the previously reported electrodes [44][45][46][47][48][49][50].…”

Electrochemical Detection of Pyocyanin as a Biomarker for Pseudomonas aeruginosa: A Focused Review

“…In another innovative approach, electrodes were printed onto the fingers of a glove to create a point-of-use electrochemical pyocyanin screening tool [ 44 ]. The glove-based sensor can be used to swipe a surface for screening of the presence of PA.…”