?-Fetoprotein in rat sera after partial hepatectomy

Dependence of the specificity of the serologic test for primary liver cancer in different areas of the world on sensitivity of the method used for detecting alpha‐fetoprotein

“…For detecting AFP in sera we used the indirect I R technique proposed by Rowe (1969Rowe ( , 1970 which we modified for double diffusion (Atelev and Elgort, 1970) and used for AFP determinations (Elgort and Abelev, 1971). In our modification, the principle of precipitation in agar with the test-system is combined with detection of the invisible precipitate with the help of 1125-labelled antiglobulin against the antibodies used to produce the precipitate.…”

“…AFP could de detected in the examined sera when its concentration was about 50 ng/ml. The technique has been described in detail earlier (Elgort and Abelev, 1971).…”

“…The proportion of positive reactions rose to 13% in viral hepatitis and to 15% in cases of metastases in the liver Abelev, 1971). To explain these clinical findings it was suggested that small amounts of AFP were produced by regenerating liver, a fact which was demonstrated in experiments with mice and rats (Abelev et al, 1963;Bakirov, 1968;Perova et al, 1971).…”

Alpha-feto-protein during development and in disease.

“…It appears that the compensatory response to neonatal hepatitis by the liver parenchyma is proliferation, retrodifferentiation, and increased synthesis of AFP, while the response to biliary atresia is proliferation of tissue incapable of AFP synthesis. A temporary reappearance of AFP in the serum following treatment with carbon tetrachloride has been noted in mice (Bakirov, 1968), rat (de Nechaud and Uriel, 1971;Perova et al, 1971), and rabbit (Branch, 1972). In mice the increased synthesis of AFP can be induced in nearly 100% of animals treated with carbon tetrachloride, but the reappearance of APP in rat and rabbits seems to be age dependent and occurs only in young animals.…”

Alpha1-Fetoprotein Biosynthesis During the Growth Cycle of Differentiated Fetal Rat Hepatocytes in Primary Monolayer Culture

“…Evidence from in vivo studies with rats suggests that this will not be the case because (a) during ontogenesis and postnatal life serum albumin levels continuously rise as a,F levels fall precipitously (33), and (b) during liver regeneration, serum aF levels rise (20,21) and there is evidence that the liver remnant might actually synthesize albumin at elevated rates (34) .…”