Alpha-feto-protein during development and in disease.

Adinolfi, A; Adinolfí, Matteo; Lessof,

doi:10.1136/jmg.12.2.138

Cited by 107 publications

(41 citation statements)

References 107 publications

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“…AFP is an oncofetal glycoprotein, and its level increases in maternal blood during gestation (15). In our study, all 72 pregnant women showed, as expected, elevated values of AFP above 20 ng/ml in serum whereas none (0%) had abnormal HCCR values above 15 g/ml (P Ͻ 0.0001).…”

Section: Discussionsupporting

confidence: 55%

The Human Cervical Cancer Oncogene Protein Is a Biomarker for Human Hepatocellular Carcinoma

Yoon

Lim²,

Ha³

et al. 2004

Cancer Research

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Human cervical cancer oncogene (HCCR) was identified and appeared to function as a negative regulator of p53 gene. The objective of this study was to validate HCCR expression as a candidate marker for human hepatocellular carcinoma. HCCR epitope was identified as Y 355 LGTRR 360 . According to immunofluorescence study, HCCR was predominantly localized in the plasma membrane and cytoplasm of hepatocellular carcinoma. HCCR proteins were overexpressed in the tumorous compared with the nontumorous cirrhosis tissues. However, HCCR was not detected in normal liver tissue. Concentration of HCCR protein in the serum was measured in a total of 570 subjects, and comparisons were made to ␣-fetoprotein. Serological studies revealed 78.2% sensitivity of HCCR (cutoff value, 15 g/ml), which was significantly higher than 64.6% of ␣-fetoprotein (P ‫؍‬ 0.0098) and 95.7% specificity for hepatocellular carcinoma. Forty of 52 (76.9%) patients with carcinoma negative for ␣-fetoprotein showed positive values for HCCR. A positive rate of 69.2% in carcinoma patients with tumor sizes <2 cm was found to be a higher rate than measurement of ␣-fetoprotein. Furthermore, HCCR expression was also detected in liver cirrhosis at an intermediate level between carcinoma and normal groups, which gave 88.1% sensitivity and 79.0% specificity using 8 g/ml as a cutoff value. In summary, the HCCR assay may have an advantage over the ␣-fetoprotein assay in that it is elevated according to disease progression from liver cirrhosis to carcinoma, and it is more frequently positive in patients with early, small hepatocellular carcinoma.

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Section: Discussionsupporting

confidence: 55%

The Human Cervical Cancer Oncogene Protein Is a Biomarker for Human Hepatocellular Carcinoma

Yoon

Lim²,

Ha³

et al. 2004

Cancer Research

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“…Girard et al previously showed that NTS is highly upregulated in HCV NS5A-expressing Huh7 cells (37) and may be associated with efficient HCV replication. The genes NTS and AFP are also known to be upregulated in hepatic tumors (38,39), and the protein SEC61G is required for tumor cell survival (40). In addition, AGR2 acts as a p53 tumor suppressor inhibitor (41).…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of an Huh.7.5.1-Derived Cell Clone Highly Permissive to Hepatitis C Virus

et al. 2015

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“…The synthesis of AFP in man and rat occurs mainly in the fetal liver and to a lesser extent in rat yolk sac (Gitlin & Boesman, 1967b). Original investigations of AFP centred on its association with primary hepatomas (see review by Abelev, 1971), but more recent studies have focused on the association of AFP with abnormal fetal development and fetal compromise in man (see review by Adinolfi, Adinolfi & Lessof, 1975).…”

Section: Introductionmentioning

confidence: 99%

Rat alpha-fetoprotein: isolation, radioimmunoassay and fetal-maternal distribution during pregnancy

Lai¹,

Forrester²,

Hancock³

et al. 1976

Reproduction

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Summary. A method is described for the isolation of mg quantities of two forms of rat alpha-fetoprotein (AFP) from amniotic fluid by preparative disc-gel column electrophoresis using a continuous elution system. AFP isolated by this method is suitable for use as an antigen, can be labelled for use in a radioimmunoassay and serves as a reference standard. The characteristics of a new antiserum to AFP are also described.The protocol for a radioimmunoassay is outlined which permits the measurement of AFP in several fetal\p=n-\maternalphysiological compartments throughout gestation. Levels of AFP in fetal liver and fetal plasma suggest that secretion of AFP from liver occurs soon after synthesis with minimal hepatic storage. The pattern for AFP in maternal serum parallels that observed in amniotic fluid and fluctuations in maternal serum levels of AFP appear to be buffered by AFP accumulation in amniotic fluid. Fetal clearance of AFP under normal conditions may be relatively constant from Days 11\p=n-\20of gestation since an amniotic fluid : maternal serum AFP ratio of 30:1 is maintained during this period.

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Alpha-feto-protein during development and in disease.

Cited by 107 publications

References 107 publications

The Human Cervical Cancer Oncogene Protein Is a Biomarker for Human Hepatocellular Carcinoma

The Human Cervical Cancer Oncogene Protein Is a Biomarker for Human Hepatocellular Carcinoma

Isolation and Characterization of an Huh.7.5.1-Derived Cell Clone Highly Permissive to Hepatitis C Virus

Rat alpha-fetoprotein: isolation, radioimmunoassay and fetal-maternal distribution during pregnancy

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