Feasibility Study of a Novel Urinary Bladder Bioreactor

Bladder substitute reconstructed in a physiological pressure environment

“…Several studies suggest the benefit of mechanical stimulation applied to engineered tissue, but only Fahrat et al demonstrated an enhancement in the organization of reseeded porcine acellular bladder with the use of such cyclic stimulation in the short term [5]. This is why we further optimized Farhat's initial concept into a bioreactor that delivers the physiological pressure implemented during a bladder cycle of continenceemicturition.…”

Constructive Remodeling of Biologic Scaffolds is Dependent on Early Exposure to Physiologic Bladder Filling in a Canine Partial Cystectomy Model

“…In vitro studies have shown that static and cyclic stretching of fibroblasts, smooth muscle cells, urothelial cells, and bone marrow derived cells seeded on an ECM scaffold lead to modulation of collagen expression, enhancements in cell and collagen alignment, and improvements in the mechanical behavior of the scaffold [15][16][17][18][19]. During in vivo remodeling, ECM scaffolds are known to degrade completely in 60 to 90 d [35].…”

“…The remodeled SIS-ECM shows contractile activity and functional innervation [4][5][6]. In addition, decellularized urinary bladder tissues have been shown to be reliable tools for studying smooth muscle cell-urothelium interactions, for designing urinary bladder bioreactors, and for repair of lower urinary tract tissues [10,11,[13][14][15].…”

Current status of tissue engineering in pediatric urology

“…Fierabracci et al [4] described the identification and characterization of bladder progenitor cells as well as expansion of this cell population, while Wallis et al [5] described the development of a urinary bladder bioreactor to aid in bladder tissue engineering. The authors attempted to simulate the hydrostatic pressures of slow bladder filling and rapid emptying by exposing the incubating seeded bladder scaffolds to mechanical transduction.…”