Current status of tissue engineering in pediatric urology

Yamzon, Jonathan; Perin, Laura; Koh, Chester J.

doi:10.1097/mou.0b013e328302f0e1

Cited by 12 publications

(6 citation statements)

References 21 publications

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“…Thus, the development of novel graft materials for urethral reconstruction is essential to develop new treatment options for remedying severe urethral defects. 2,3,5,6 The selection of an appropriate biomaterial for the application is crucial, and the biomaterial for urethral reconstruction should meet the requirements of being biocompatible, nontoxic, biodegradable without disadvantageous tissue reactions and able to promote urothelial tissue regeneration. Furthermore, for urethral reconstruction, the biomaterial should be elastic and flexible and should mimic the basement membrane of the urothelium, generating a suitable matrix for urothelial cells to attach and proliferate.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Poly(l-lactide-co-ɛ-caprolactone) and Poly(trimethylene carbonate) Membranes for Urethral Regeneration: AnIn VitroandIn VivoStudy

Sartoneva

Nordback

Haimi

et al. 2018

Tissue Engineering Part A

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Urethral defects are normally reconstructed using a patient's own genital tissue; however, in severe cases, additional grafts are needed. We studied the suitability of poly(l-lactide-co-ɛ-caprolactone) (PLCL) and poly(trimethylene carbonate) (PTMC) membranes for urethral reconstruction in vivo. Further, the compatibility of the materials was evaluated in vitro with human urothelial cells (hUCs). The attachment and viability of hUCs and the expression of different urothelial cell markers (cytokeratin 7, 8, 19, and uroplakin Ia, Ib, and III) were studied after in vitro cell culture on PLCL and PTMC. For the in vivo study, 32 rabbits were divided into the PLCL (n = 15), PTMC (n = 15), and control or sham surgery (n = 2) groups. An oval urethral defect 1 × 2 cm in size was surgically excised and replaced with a PLCL or a PTMC membrane or urethral mucosa in sham surgery group. The rabbits were followed for 2, 4, and 16 weeks. After the follow-up, urethrography was performed to check the patency of the urethra. The defect area was excised for histological examination, where the epithelial integrity and structure, inflammation, and fibrosis were observed. There was no notable difference on hUCs attachment on PLCL and PTMC membranes after 1 day of cell seeding, further, the majority of hUCs were viable and maintained their urothelial phenotype on both biomaterials. Postoperatively, animals recovered well, and no severe strictures were discovered by urethrography. In histological examination, the urothelial integrity and structure developed toward a normal urothelium with only mild signs of fibrosis or inflammation. According to these results, PLCL and PTMC are both suitable for reconstructing urethral defects. There were no explicit differences between the PLCL and PTMC membranes. However, PTMC membranes were more flexible, easier to suture and shape, and developed significant epithelial integrity.

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Section: Introductionmentioning

confidence: 99%

Comparison of Poly(l-lactide-co-ɛ-caprolactone) and Poly(trimethylene carbonate) Membranes for Urethral Regeneration: AnIn VitroandIn VivoStudy

Sartoneva

Nordback

Haimi

et al. 2018

Tissue Engineering Part A

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“…Unipotent stem cells can give rise to only one distinct cell type, such as epithelial cells; these cells have a limited capacity for self-renewal and labeling then as stem cells has been debated [46]. Ethical concerns regarding the use of ESCs have arisen due to the need to destroy embryos to isolate such cells.…”

Section: Classification Of Stem Cellsmentioning

confidence: 99%

The Current Status of Stem-Cell Therapy in Erectile Dysfunction: A Review

Reed‐Maldonado

Lue

2016

World J Mens Health

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Stem cells are undifferentiated cells that are capable of renewal and repair of tissue due to their capacity for division and differentiation. The purpose of this review is to describe recent advances in the use of stem cell (SC) therapy for male erectile dysfunction (ED). We performed a MEDLINE database search of all relevant articles regarding the use of SCs for ED. We present a concise summary of the scientific principles behind the usage of SC for ED. We discuss the different types of SCs, delivery methods, current pre-clinical literature, and published clinical trials. Four clinical trials employing SC for ED have been published. These articles are summarized in this review. All four report improvements in ED after SC therapy. SC therapy remains under investigation for the treatment of ED. It is reassuring that clinical trials thus far have reported positive effects on erectile function and few adverse events. Safety and methodical concerns about SC acquisition, preparation and delivery remain and require continued investigation prior to wide-spread application of these methods.

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“…In tissue engineering, a tissue or an organ is grown through cell implantation or the growth of host cells [ 10 ]. Mechanical features similar to the native tissue, easy manipulation, biocompatibility, and negligible or absent immunological reactions are the most important criteria for choosing the ideal scaffold and cell type in seeded grafts [ 11 – 15 ]. Some of the appropriate natural scaffolds in tissue engineering of bladder reconstruction are natural polymers, small intestinal submucosa (SIS), bladder acellular matrix (BAM), amniotic membrane, and dermis [ 16 – 20 ].…”

Section: Introductionmentioning

confidence: 99%

Current evidence regarding alternative techniques for enterocystoplasty using regenerative medicine methods: a systematic review

Arabzadeh Bahri,

Peisepar,

Maleki

et al. 2024

Eur J Med Res

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Enterocystoplasty is the most commonly used treatment for bladder reconstruction. However, it has some major complications. In this study, we systematically reviewed the alternative techniques for enterocystoplasty using different scaffolds. A comprehensive search was conducted in PubMed, Embase, and Cochrane Library, and a total of 10 studies were included in this study. Five different scaffolds were evaluated, including small intestinal submucosa (SIS), biodegradable scaffolds seeded with autologous bladder muscle and urothelial cells, dura mater, human cadaveric bladder acellular matrix graft, and bovine pericardium. The overall results revealed that bladder reconstruction using regenerative medicine is an excellent alternative method to enterocystoplasty regarding the improvement of bladder capacity, bladder compliance, and maximum detrusor pressure; however, more large-scale studies are required.

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Current status of tissue engineering in pediatric urology

Cited by 12 publications

References 21 publications

Comparison of Poly(l-lactide-co-ɛ-caprolactone) and Poly(trimethylene carbonate) Membranes for Urethral Regeneration: AnIn VitroandIn VivoStudy

Comparison of Poly(l-lactide-co-ɛ-caprolactone) and Poly(trimethylene carbonate) Membranes for Urethral Regeneration: AnIn VitroandIn VivoStudy

The Current Status of Stem-Cell Therapy in Erectile Dysfunction: A Review

Current evidence regarding alternative techniques for enterocystoplasty using regenerative medicine methods: a systematic review

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