Bladder substitute reconstructed in a physiological pressure environment

Bouhout, Sara; Gauvin, Robert; Gibot, Laure; Aubé, David; Bolduc, Stéphane

doi:10.1016/j.jpurol.2011.03.002

Cited by 28 publications

(22 citation statements)

References 20 publications

(18 reference statements)

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“…Cells are allowed to proliferate for one week; then the equivalent is to be cultured at the air/liquid interface for three more weeks. By preconditioning the reconstructed tissue in a dynamic environment to simulate the filling and emptying cycles of the bladder, the use of bioreactor may enhance the mechanical behavior of the extracellular matrix [47]. Moreover, it has been demonstrated that a dynamic environment can lead to a better differentiation of the urothelial cell compared to static one [48].…”

Section: Tissue Engineering Of Urinary Bladdermentioning

confidence: 99%

Tissue Engineering of Urinary Bladder and Urethra: Advances from Bench to Patients

Orabi

Bouhout

Morissette

et al. 2013

The Scientific World Journal

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Urinary tract is subjected to many varieties of pathologies since birth including congenital anomalies, trauma, inflammatory lesions, and malignancy. These diseases necessitate the replacement of involved organs and tissues. Shortage of organ donation, problems of immunosuppression, and complications associated with the use of nonnative tissues have urged clinicians and scientists to investigate new therapies, namely, tissue engineering. Tissue engineering follows principles of cell transplantation, materials science, and engineering. Epithelial and muscle cells can be harvested and used for reconstruction of the engineered grafts. These cells must be delivered in a well-organized and differentiated condition because water-seal epithelium and well-oriented muscle layer are needed for proper function of the substitute tissues. Synthetic or natural scaffolds have been used for engineering lower urinary tract. Harnessing autologous cells to produce their own matrix and form scaffolds is a new strategy for engineering bladder and urethra. This self-assembly technique avoids the biosafety and immunological reactions related to the use of biodegradable scaffolds. Autologous equivalents have already been produced for pigs (bladder) and human (urethra and bladder). The purpose of this paper is to present a review for the existing methods of engineering bladder and urethra and to point toward perspectives for their replacement.

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Section: Tissue Engineering Of Urinary Bladdermentioning

confidence: 99%

Tissue Engineering of Urinary Bladder and Urethra: Advances from Bench to Patients

Orabi

Bouhout

Morissette

et al. 2013

The Scientific World Journal

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“…In order to be sure that the tissues produced could be easily manipulated and be able to withstand physiological conditions, several parameters were tested for SS and RS stromas. The ultimate tensile strength (Figure C) of RS tissues was inferior to that of SS‐made tissues, but remained superior to the value found previously for native porcine bladder (0.32 MPa) (Bouhout et al ., ). The deformation, elastic modulus and maximal strength values of SS and RS stromas were similar, without any statistically significant differences.…”

Section: Resultsmentioning

confidence: 97%

“…As the cells build their microenvironment through matrix deposition, it could be expected that tissues, free of exogenous materials, will show matrix–cell and cell–cell interactions that more accurately reproduce the native tissue. Through the years, this technique was extended to research on several tissues, such as cornea (Proulx et al ., ), hypodermis (Vermette et al ., ), bladder (Magnan et al ., ; Bouhout et al ., ; Bouhout et al ., ), urethra (Magnan et al ., ; Cattan et al ., ) and skin with physiological or pathological features [psoriasis (Jean et al ., ), scleroderma (Corriveau et al ., ), hypertrophic scarring (Simon et al ., ) or melanoma (Auger et al ., )]. Moreover, from a clinical point of view, this technique is also used on a small scale for the treatment of burned patients (Auger et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Inexpensive production of near-native engineered stromas

Chabaud

Rousseau

Marcoux

et al. 2015

J Tissue Eng Regen Med

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Although the self-assembly approach is an efficient method for the production of engineered physiological and pathological tissues, avoiding the use of exogenous materials, it nevertheless remains expensive and requires dexterity, which are features incompatible with large-scale production. We propose a modification to this technique to make easier the production of mesenchymal compartment, to reduce the cost and to improve the histological quality of the self-assembled tissues. The stroma produced by this novel approach allowed epithelial cell differentiation, resulting in a pseudostratified epithelium that shared several features with native tissues. The incorporation of endothelial cells in the reconstructed mesenchyme formed a three-dimensional capillary-like network, positive for CD31 and von Willebrand factor and surrounded by NG2 positive cells. It could limit self-contraction of the resulting tissue by recruiting α-Smooth Muscle Actin positive cells. With this new technique, which is relatively inexpensive and easy to use in a research laboratory set-up, near-native stromas can now be produced with minimal handling time. Copyright © 2015 John Wiley & Sons, Ltd.

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“…However, the stretch produced by these devices is not physiologically relevant. Several studies have demonstrated that a mechanical system imposed during the bladder filling-emptying cycle can improve biological functions of the cell [12,13]. In this study, we describe novel biaxial stretch cycles that regulate HBSMCs proliferation and function in a modified BOSE BioDynamic system, which may have potential to generate functional HBSMCs for tissue engineering.…”

Section: Discussionmentioning

confidence: 97%

Magnitude-dependent proliferation and contractility modulation of human bladder smooth muscle cells under physiological stretch

Luo

Wazir

et al. 2015

World J Urol

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Bladder substitute reconstructed in a physiological pressure environment

Abstract: This new alternative method offers a promising avenue for regenerative medicine. It is distinguished by its autologous character and its efficiency as a barrier to urea. These properties could significantly reduce inflammation, necrosis, and therefore, possible rejection.

Cited by 28 publications

References 20 publications

Tissue Engineering of Urinary Bladder and Urethra: Advances from Bench to Patients

Tissue Engineering of Urinary Bladder and Urethra: Advances from Bench to Patients

Inexpensive production of near-native engineered stromas

Magnitude-dependent proliferation and contractility modulation of human bladder smooth muscle cells under physiological stretch

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