Constructive Remodeling of Biologic Scaffolds is Dependent on Early Exposure to Physiologic Bladder Filling in a Canine Partial Cystectomy Model

Boruch, Alan V.; Nieponice, Alejandro; Qureshi, Irfan; Gilbert, Thomas W.; Badylak, Stephen F.

doi:10.1016/j.jss.2009.02.014

Cited by 68 publications

(51 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…20 The temporal appearance of these cell phenotypes is likely dependent upon anatomic site, 45 microenvironmental niche factors, 51 and epigenetic factors such as mechanical forces. 52 Degradation products of ECM scaffolds have been shown to include chemotactic factors for myogenic progenitor cells. 47,53,54 Thus, it is possible that a subset of the dense mononuclear cells includes progenitor cells with myogenic potential.…”

Section: Discussionmentioning

confidence: 99%

A Murine Model of Volumetric Muscle Loss and a Regenerative Medicine Approach for Tissue Replacement

Sicari

Agrawal

Siu

et al. 2012

Tissue Engineering Part A

Self Cite

143

123

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

A Murine Model of Volumetric Muscle Loss and a Regenerative Medicine Approach for Tissue Replacement

Sicari

Agrawal

Siu

et al. 2012

Tissue Engineering Part A

Self Cite

143

123

View full text Add to dashboard Cite

“…33 The specific molecules within the UBM degradation products that are responsible for the effects reported herein are unknown. ECM facilitates constructive remodeling in a variety of tissues, 29,[34][35][36][37][38][39] and endogenous progenitor/stem cells participate in this process. 6,40 Bioactive degradation products of ECM promote the migration of a variety of cell types, including terminal Schwann cells, 41 endothelial cells, 42 and the perivascular stem cell population used in this present study.…”

Section: Discussionmentioning

confidence: 99%

Extracellular Matrix Degradation Products and Low-Oxygen Conditions Enhance the Regenerative Potential of Perivascular Stem Cells

Tottey

Corselli

Jeffries

et al. 2011

Tissue Engineering Part A

Self Cite

View full text Add to dashboard Cite

Tissue and organ injury results in alterations of the local microenvironment, including the reduction in oxygen concentration and degradation of the extracellular matrix (ECM). The response of perivascular stem cells to these microenvironment changes are of particular interest because of their wide distribution throughout the body and their potential involvement in tissue and organ response to injury. The chemotactic, mitogenic, and phenotypic responses of this stem cell population were evaluated in response to a combination of decreased oxygen concentration and the presence of ECM degradation products. Culture in low-oxygen conditions resulted in increased proliferation and migration of the cells and increased activation of the ERK signaling pathway and associated integrins without a change in cell surface marker phenotype. The addition of ECM degradation products were additive to these processes. Reactive oxygen species within the cells were increased in association with the mitogenic and chemotactic responses. The increased proliferation and chemotactic properties of this stem cell population without any changes in phenotype and differentiation potential has important implications for both in vitro cell expansion and for in vivo behavior of these cells at the site of injury.

show abstract

“…Color images available online at www.liebertonline.com/tea rived from the porcine urinary bladder has been used in multiple pre-clinical applications for site-specific remodeling of a variety of soft tissues. [54][55][56][57][58][59][60][61][62][63] However, commercially available biologic scaffolds composed of ECM are derived from a number of species and organs. 64 It is possible, and in fact likely, that a similar cryptic peptide would be found in ECMs from other sources.…”

Section: Figmentioning

confidence: 99%

Recruitment of Progenitor Cells by an Extracellular Matrix Cryptic Peptide in a Mouse Model of Digit Amputation

Agrawal

Tottey

Johnson

et al. 2011

Tissue Engineering Part A

Self Cite

159

136

View full text Add to dashboard Cite

Biologic scaffolds composed of extracellular matrix (ECM) have been used successfully in preclinical models and humans for constructive remodeling of functional, site-appropriate tissue after injury. The mechanisms underlying ECM-mediated constructive remodeling are not completely understood, but scaffold degradation and sitedirected recruitment of both differentiated and progenitor cells are thought to play critical roles. Previous studies have shown that degradation products of ECM scaffolds can recruit a population of progenitor cells both in vitro and in vivo. The present study identified a single cryptic peptide derived from the a subunit of the collagen III molecule that is chemotactic for a well-characterized perivascular stem cell in vitro and causes the site-directed accumulation of progenitor cells in vivo. The oligopeptide was additionally chemotactic for human cortical neural stem cells, rat adipocyte stem cells, C2C12 myoblast cells, and rat Schwann cells in vitro. In an adult murine model of digit amputation, treatment with this peptide after mid-second phalanx amputation resulted in a greater number of Sox2 + and Sca1 + ,Lin -cells at the site of injury compared to controls. Since progenitor cell activation and recruitment are key prerequisites for epimorphic regeneration in adult mammalian tissues, endogenous site-directed recruitment of such cells has the potential to alter the default wound healing response from scar tissue toward regeneration.

show abstract

Constructive Remodeling of Biologic Scaffolds is Dependent on Early Exposure to Physiologic Bladder Filling in a Canine Partial Cystectomy Model

Cited by 68 publications

References 40 publications

A Murine Model of Volumetric Muscle Loss and a Regenerative Medicine Approach for Tissue Replacement

A Murine Model of Volumetric Muscle Loss and a Regenerative Medicine Approach for Tissue Replacement

Extracellular Matrix Degradation Products and Low-Oxygen Conditions Enhance the Regenerative Potential of Perivascular Stem Cells

Recruitment of Progenitor Cells by an Extracellular Matrix Cryptic Peptide in a Mouse Model of Digit Amputation

Contact Info

Product

Resources

About