Fast high-throughput screening of angiotensin-converting enzyme insertion/deletion polymorphism by variable programmed electric field strength-based microchip electrophoresis

Sun, Yucheng; Kim, Su-Kang; Zhang, Peng; Woo, Nain; Kang, Seong Ho

doi:10.1016/j.jchromb.2016.06.007

Cited by 6 publications

(9 citation statements)

References 34 publications

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“…However, the PCR products were introduced into three parallel channels (multichannel chip 1, Figure (b)) and five parallel channels (multichannel chip 2, Figure (c)) separately. As the fabrication of multichannel microchip 1 was not same as those of the other two types regarding detection points and channel lengths, the separation conditions were slightly different . In both of the multichannel microchips, different genotypes with 10 PCR cycles and 35 cycles could be detected simultaneously in a short‐time (<25 s) without loss of resolving power ( R s ≥ 1.8), and the S / N ratio increased with increasing number of PCR cycles (Figures and S3, Tables S2 and S3).…”

Section: Application Of the Me‐pefs Methods To Human Bloodmentioning

confidence: 99%

“…The sample injection was performed by applying the same potential discussed above. As the fabrication of multichannel chip 1 was different from that of the single‐channel chip, the separation process was set as 615 V/cm, 0–22.5 s; 231 V/cm, 22.5–28.5 s; and 615 V/cm, 28.5–40 s . After each run, the microchip was rinsed with pure water for 10 min.…”

Section: Methodsmentioning

confidence: 99%

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Fast High‐Throughput Screening of Alzheimer's Disease by Direct Apolipoprotein E Genotyping‐based Multichannel Microchip Electrophoresis

Sun

Woo

Kim

et al. 2017

Bulletin Korean Chem Soc

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Section: Application Of the Me‐pefs Methods To Human Bloodmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Fast High‐Throughput Screening of Alzheimer's Disease by Direct Apolipoprotein E Genotyping‐based Multichannel Microchip Electrophoresis

Sun

Woo

Kim

et al. 2017

Bulletin Korean Chem Soc

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“…Acute myocardial infarction [122,123], coronary artery disease [124][125][126][127][128], and hypertension [129] were investigated.…”

Section: Cardiovascular Diseasesmentioning

confidence: 99%

“…The insertion and deletion polymorphism in the angiotensin-converting enzyme gene is associated with hypertension. A strategy of MGE-LIF using an electric-field gradient was explored for mutation detection in the Alu element of the angiotensin-converting enzyme gene [129]. The target DNA had a length of 190 bp and 490 bp after deletion and insertion, respectively.…”

Section: Hypertensionmentioning

confidence: 99%

A decade of microchip electrophoresis for clinical diagnostics – A review of 2008–2017

Wuethrich

Quirino

2019

Analytica Chimica Acta

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A core element in clinical diagnostics is the data interpretation obtained through the analysis of patient samples. To obtain relevant and reliable information, a methodological approach of sample preparation, separation, and detection is required. Traditionally, these steps are performed independently and stepwise. Microchip capillary electrophoresis (MCE) can provide rapid and high-resolution separation with the capability to integrate a streamlined and complete diagnostic workflow suitable for the point-of-care setting. Whilst standard clinical diagnostics methods normally require hours to days to retrieve specific patient data, MCE can reduce the time to minutes, hastening the delivery of treatment options for the patients. This review covers the advances in MCE for disease detection from 2008 to 2017. Miniaturised diagnostic approaches that required an electrophoretic separation step prior to the detection of the biological samples are reviewed. In the two main sections, the discussion is focused on the technical setup used to suit MCE for disease detection and on the strategies that have been applied to study various diseases. Throughout these discussions MCE is compared to other techniques to create context of the potential and challenges of MCE. A comprehensive table categorised based on the studied disease using MCE is provided. We also comment on future challenges that remain to be addressed.

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“…Rigat et al were the rst to describe a method for the detection of I/D polymorphisms using polymerase chain reaction (PCR) and agarose gel electrophoresis [1,2]. Other techniques including real-time polymerase chain reaction (RT-PCR) [3][4][5], high resolution melting (HRM) [6,5], polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) [7] and microchip electrophoresis (ME) system [8] have also been used to detect I/D polymorphism. However, these techniques require tedious experimental procedures and expensive and sophisticated instruments that may not be available in clinical institutions.…”

Section: Introductionmentioning

confidence: 99%

A Novel PCR Method for Detecting ACE Gene Insertion/Deletion Polymorphisms and Its Clinical Application

Yang¹,

Liang²,

Huang³

et al. 2020

Preprint

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Background: Angiotensin-converting enzyme (ACE) plays a major role in blood pressure regulation and cardiovascular homeostasis. The wide distribution and multifunctional properties of ACE suggest it’s involvement in various pathophysiological conditions. Results: In this study, a novel visual detection method for ACE I/D polymorphisms was designed by integrating direct PCR without the need for DNA extraction using gold magnetic nanoparticles (GMNPs)-based lateral flow assay (LFA) biosensor. The entire detection procedure could enable the genotyping of clinical samples in about 80 minutes. The detection limit was 0.75 ng and results could be obtained in 5 minutes using the LFA device. Three hundred peripheral blood samples were analyzed using the direct PCR-LFA system and then verified by sequencing to determine accuracy and repeatability. A clinical preliminary study was then performed to analyze a total of 633 clinical samples. Conclusions: After grouping based on age, we found a significant difference between the genotypes and the age of patients in the CHD group. The introduction of this method into clinical practice may be helpful for the diagnosis of diseases caused by large fragment gene insertions/deletions.

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Fast high-throughput screening of angiotensin-converting enzyme insertion/deletion polymorphism by variable programmed electric field strength-based microchip electrophoresis

Cited by 6 publications

References 34 publications

Fast High‐Throughput Screening of Alzheimer's Disease by Direct Apolipoprotein E Genotyping‐based Multichannel Microchip Electrophoresis

Fast High‐Throughput Screening of Alzheimer's Disease by Direct Apolipoprotein E Genotyping‐based Multichannel Microchip Electrophoresis

A decade of microchip electrophoresis for clinical diagnostics – A review of 2008–2017

A Novel PCR Method for Detecting ACE Gene Insertion/Deletion Polymorphisms and Its Clinical Application

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