Fast High‐Throughput Screening of Alzheimer's Disease by Direct Apolipoprotein E Genotyping‐based Multichannel Microchip Electrophoresis

Abstract

The apolipoprotein E polymorphism genes of Alzheimer's disease were detected within 20 s by multichannel microchip electrophoresis after polymerase chain reaction amplification from whole blood without DNA extraction.

Fast and highly sensitive screening of PCR products of severe acute respiratory syndrome coronavirus 2 by voltage‐programmed capillary gel electrophoretic separation

“…Even if the voltage changes, the value of μ 2 − μ 1 is the same, but as the applied voltage V increases, the absolute value of the electrophoretic mobility increases, resulting in a loss of R s as the μ EP value increases. Therefore, the VP method is required for the fast analysis of DNA molecules without the loss of resolution 29,30 …”

“…Therefore, the VP method is required for the fast analysis of DNA molecules without the loss of resolution. 29,30 In this study, a CGE with a laser-induced fluorescence (LIF) detector was used for highly sensitive COVID-19 screening with a small amount of sample (Figure 1b). Especially, the CGE method with a LIF detector based on VP showed a fast and highly sensitive detection of DNA molecules without resolving power, while minimizing Joule heating generated when a high electric field was applied under separation sieving gel in the CGE.…”