Faecalibacter macacae gen. nov., sp. nov., isolated from the faeces of Macaca assamensis

Abstract

Huge numbers of bacteria reside in the digestive tract of most animals. During an investigation into the bacterial diversity of primates, strain YIM 102668T was isolated. When neighbour-joining phylogenetic analysis based on 16S rRNA gene sequences was conducted, strain YIM 102668T formed a cluster within the family Flavobacteriaceae and in a lineage not associated with any known group of previously proposed genera. Closely related genera were Algoriella (94.8 %), Chishuiella (94.8 %), Empedobacter (highest 94.6 %), Moheibacter (90.9 %) and Weeksella (90.6 %). In addition, strain YIM 102668T contained MK-6 as the predominant respiratory quinone and iso-C15 : 0 as the major fatty acid. The major polar lipid was phosphatidylethanolamine and the genomic DNA G+C content was 30.6 mol%. These chemotaxonomic characterizations confirmed that strain YIM 102668T belonged to the family Flavobacteriaceae . Supported by the results of phylogenetic, phenotypic and chemotaxonomic analyses, we propose that strain YIM 102668T represents a novel genus, for which the name Faecalibacter macacae gen. nov., sp. nov. is proposed. The type strain is YIM 102668T (=KCTC 52109T=CCTCC AB 2016016T).

Faecalibacter bovis sp. nov., isolated from cow faeces

“…S4. The major polar lipid of strain ZY171143 T and the six reference type strains was phosphatidylethanolamine (PE), and major polar lipid PE of the six reference type strains were uniform with previous reports [10, 35, 36]. The polar lipid that distinguished strain ZY171143 T from Faecalibacter macacae CCTCC AB 2016016 T , Algoriella xinjiangensis CGMCC 1.10229 T , Chishuiella changwenlii CGMCC 1.12707 T and Empedobacter brevis CCUG 7320 T was lysophosphatidylcholine (LPC) (, Fig.…”

“…At the time of writing, the family comprises of 22 validly genera (https://lpsn.dsmz.de/family/Weeksellaceae). Most type species of the genera in the family were isolated from humans [1–5] and animals [6–10]. Some species in the family, such as Empedobacter brevis [11], Elizabethkingia anopheles [12], Riemerella anatipestifer [13], Ornithobacterium rhinotracheale [14], Bergeyella zoohelcum [15], Chryseobacterium meningosepticum [16] and Weeksella virosa [17] are important pathogens of animals and/or humans.…”

“…The shapes of the six reference type strains were rigid rod or oval-rod (). Humps were not observed on the cell surface of strain ZY171143 T (), but were observed on the cells of strain Faecalibacter macacae CCTCC AB 2016016 T [10]. The cell shape, size and humps could be used to clearly distinguish strain ZY171143 T from Faecalibacter macacae CCTCC AB 2016016 T and the other five reference types strains.…”

Faecalibacter rhinopitheci sp. nov., a bacterium isolated from the faeces of Rhinopithecus bieti

“…At the time of writing, about 148 genera of the family Flavobacteriaceae from various environments had been isolated and listed with validly published names (https://lpsn.dsmz.de/family/flavobacteriaceae). A novel genus of the family Flavobacteriaceae , Faecalibacter , was recently proposed by Chen et al [3]. However, there is only one species, Faecalibacter macacae gen. nov., sp.…”

“…However, there is only one species, Faecalibacter macacae gen. nov., sp. nov., in the genus, which was isolated from the faeces of Macaca assamensis [3]. Members of the genus Faecalibacter are characterized as Gram-stain-negative, non-motile, producing yellow colonies and oval–rod-shaped cells; the predominant isoprenoid quinone and the major cellular fatty acid are menaquinone 6 and iso-C 15 : 0 , respectively.…”