Faecalibacter rhinopitheci sp. nov., a bacterium isolated from the faeces of Rhinopithecus bieti

Abstract

A novel Gram-stain-negative strain, WQ 117T, isolated from the faeces of Rhinopithecus bieti collected at Yunnan Snub-nosed Monkey National Park, Yunnan province, PR China, was subjected to a polyphasic taxonomic study. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate represented a member of the genus Faecalibacter , sharing 97.64 % sequence similarity with the type strain Faecalibacter macacae YIM 102668T. The G+C content of the genomic DNA of WQ117T was 30.5 mol%. The predominant isoprenoid quinone was MK-6. The major cellular fatty acids was iso-C15 : 0. The whole genome average nucleotide identity (gANI) values and the digital DNA–DNA hybridization values between WQ 117T and YIM 102668T were 79.66 % and 22.20 %, respectively. Growth occurred at 0–50 °C (optimally at 28–35 °C), pH 7.0–9.0 (optimally at pH 8.0) and with 0–2 % (w/v) NaCl (optimally without NaCl). On the basis of the taxonomic evidence, a novel species, Faecalibacter rhinopitheci sp. nov., is proposed. The type strain is WQ 117T (=KCTC 82394T=CCTCC AA 2020027T).

Metagenomic and culture-dependent analysis of Rhinopithecius bieti gut microbiota and characterization of a novel genus of Sphingobacteriaceae

“…From the obtained pure cultures, it was found that the similarities of 16S rRNA gene sequences of 20 strains were less than or equal to 98.7% when compared to those of their most related strains (one of the criteria of new species classification) 67 , indicating that these strains were potential new taxa (Table S7 ). Among them, WQ 047 and WQ 117 have been identified as new taxon and validly published 24 , 25 , and renamed as Sphingobacterium Rhinopitheci WQ 047 T and Faecalibacter Rhinopitheci WQ117 T , respectively. Among the remaining species, WQ 2009 T has the lowest sequence similarity with its most similar species.…”

“…Through serial dilutions with 0.1% sterilized Na 4 P 2 O 7 , samples were spread on Columbia Agar plates (Hopebio, China), and were cultured at 30 °C for at least 7 days. The pure culture was obtained as described previously 24 , 25 and stored at 4 °C for further study. These pure isolates were cultivated on Columbia or LB agar at 30 °C unless otherwise stated.…”

“…In this study, metagenomic analysis was conducted to elucidate the diversity of gut microbes and the profiles of carbohydrate-active enzymes from R. bieti . Culture-dependent analysis was also carried out to investigate the faecal microbes of R. bieti , and a series of new species have been isolated and characterized 24 , 25 . At present, a bacterium designated as WQ 2009 T was isolated, and the strain represented a novel genus of the family Sphingobacteriaceae based on detailed polyphasic studies.…”

Paenibacillus baimaensis sp. nov., a bacterium isolated from mountain soil in the habitat of Rhinopithecus bieti

“…Fatty acids were saponified, methylated, extracted and analysed following the midi technical note [27] with an Agilent 7890A instrument with the midi System (Sherlock version 6.1; database TSBA6). Polar lipids were extracted with chloroform–methanol and analysed by two-dimensional TLC described by Wang et al [28]. Menaquinone was prepared and analysed by HPLC (Agilent 1260) following the method described by Tamaoka [29].…”

Paenibacillus polygoni sp. nov., an endophytic bacterium isolated from Polygonum lapathifolium L. in wetland

“…Anaerobic growth was assessed in the anaerobic glovebox at 30 °C for 3 days. Catalase activity and oxidase activity were tested using the methods described by Wang et al [44]. Additionally, other physiological properties were test using API 20NE, API 50CHB and API ZYM systems (bioMérieux).…”