Faecalibacter bovis sp. nov., isolated from cow faeces

Abstract

A Gram-stain-negative, non-spore-forming, yellow-pigmented, aerobic, pleomorphic rod-shaped bacterium, designated ZY171143T, was isolated from faeces of a cow with diarrhoea in Wenshan, Yunnan Province, south-west China and its taxonomic position was studied. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZY171143T belonged to the family Weeksellaceae and was most closely related to the only species of the genus Faecalibacter , Faecalibacter macacae CCTCC AB 2016016T with a sequence similarity of 97.8 %. The genomic OrthoANI and digital DNA–DNA hybridization values between the strain and F. macacae CCTCC AB 2016016T were 86.2 and 30.5 %, respectively. The genomic G+C content was 31.1 mol%. The predominant fatty acids (>5 %) were C15 : 0 iso, C17 : 0 iso 3OH, C16 : 0, C16 : 1 ω5c and summed feature 3 (C16 : 1 ω7c and/or 16 : 1 ω6c). The major polar lipids were phosphatidylethanolamine, triacylglycerol and sulfonolipid. The sole respiratory quinone was MK-6. These chemotaxonomic characterizations also revealed that strain ZY171143T was a member of the genus Faecalibacter . Based on the phenotypic, chemotaxonomic and genotypic data, strain ZY171143T represents a novel species within the genus Faecalibacter , for which the name Faecalibacter bovis sp. nov. is proposed. The type strain is ZY171143T (=CGMCC 1.13663T=KCTC 62642T).

Moraxella nasovis sp. nov., isolated from a sheep with respiratory disease

“…The fatty acids of strain ZY201115 T and the four reference type strains, including M. bovis CCUG 2133 T , M. ovis CCUG 354 T , M. lacunata CCUG 4441 T and M. cuniculi CCUG 2154 T , were analysed at the China General Microbiological Culture Collection Center (CGMCC, Beijing, PR China) by using the Sherlock Microbial Identification System version A 5.05 (midi) following midi's instructions, and identified using the TSBA6.0 database [47]. The polar lipids of strain ZY201115 T and the four reference type strains were identified at a lipid identification company (Profleader Biotech Co.,Ltd., Shanghai, PR China) by using ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap mass spectrometry system (Thermo Fisher Scientific), with the relative quantification method described by Li et al [48]. The respiratory quinones of strain ZY201115 T and the four reference type strains were identified at the CGMCC with the method described previously [49], and the Shewanella schlegelianan (CGMCC 1.8480) was chosen as the reference strain.…”