Factors Influencing Changes in pH during Storage of Platelet Concentrates at 20–24°C 1, 2

To study the influence of contaminating leukocytes on the storage conditions of platelet concentrates (PC), various amounts of leukocytes were added to identical PC. From 12 blood donations, 12 leukocyte-poor PC were prepared and pooled. Subsequently, the pool was divided into 12 identical PC. The plasma volume of the PC was 58.6±0.6 ml, the platelet concentration was 1.01±0.04x10^9/ml (mean ± SD) and the red cell contamination did not exceed 10^7 per PC. To 4 groups of 3 PC, pooled leukocytes were added from the same 12 blood donations. The leukocyte contamination for each group of 3 PC was 0.14±0.05, 1.96±0.09, 5.53±0.98 and 13.0±0.93x10^6/ml (mean ± SD) for groups I-IV, respectively. The PC were stored for 7 days at 22 °C in normal polyvinylchloride bags. A significant correlation was found between increasing concentrations of leukocytes in the PC and the drop in pH (r=-0.93), glucose consumption (r=-0.91), lactic acid production (r=0.93) and release of lactate dehydrogenase (r=0.92) during storage of the PC. The excretion of β-thromboglobulin, depletion of platelet adenine nucleotides, decreased ability to incorporate 3H-adenosine into metabolic nucleotides and poor morphology of the platelets were also significantly correlated with an increased number of leukocytes in the PC. These data show that high concentrations of leukocytes in PC have a significant detrimental effect on the viability of platelets during storage at 22 °C. We conclude that for good storage conditions of PC, the upper limit of leukocytes per PC should not exceed 10^7.

mentioning

confidence: 95%

Preparation of Leukocyte-Poor Platelet Concentrates from Buffy Coats

Pietersz¹,

Korte²,

Reesink³

et al. 1988

“…Several authors [14]have reported an inverse relationship between post storage pH and platelet concentration in 50 ml plasma, while others [20]indicated the lack of a strict relationship. We recently reported [12]that the total number of platelets and pH had a close correlation (r = –0.86) when stored in specific polyolefin containers.…”

Section: Discussionmentioning

confidence: 99%

“…Beutler and Kuhl [19]demonstrated that the presence of leukocytes has an appreciable effect on lactate production and on the fall of pH of PCs stored in first generation containers. Moroff et al [20]showed that the amount of residual leukocytes was not contributing. In this study, the residual number of leukocytes was very low; 45 of 67 PCs showed a residual number of leukocytes below 5 × 10 6 /U.…”

Section: Discussionmentioning

confidence: 99%

“…like the residual leukocyte counts [19, 20], platelet concentration [1, 2, 4], acetate metabolism [21, 22, 23]and the buffer capacity of the storage medium, particularly its bicarbonate content [20, 21, 22, 23]. Beutler and Kuhl [19]demonstrated that the presence of leukocytes has an appreciable effect on lactate production and on the fall of pH of PCs stored in first generation containers.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Platelets Stored in a New-Generation ContainerDifferences between Plasma and Platelet Additive Solution II

Wildt‐Eggen¹,

Schrijver²,

Smid³

et al. 1998

Background and Objectives: In order to preserve platelet concentrates (PCs) with high yields, a new polyolefin platelet storage container (PL 2410, 1.3L, Baxter, La Châtre, France) with increased gas permeability in combination with a larger surface area has been developed. The storage capacity was studied with platelets in plasma and platelets additive solution. Materials and Methods: Platelet concentrate pools (PCs) of different yields suspended in either plasma (PCs-PL; n = 30) or PAS II (PCs-PAS; n = 37) were prepared. For preparation of PCs with a low, intermediate and high number of platelets 3, 5 and 6 buffy coat (BCs) were pooled with different volumes of plasma and 5 and 6 BCs were pooled with different volumes of PAS II, in order to obtain PCs of equal volumes comparable with routine conditions. All PCs were stored on a flatbed shaker at 22±2 °C and evaluated on days 1, 2, 5 and 7 by determining platelet and white cell counts, pH (37 °C), pO₂, pCO₂ and swirling score. Results: Platelet yields ranged from 1.5 up to 5.5 × 10¹¹ platelets/U. On day 7 all PCs-PL (n = 4) with platelet yields above 4.5 × 10¹¹/U had a pH value below 6.8 (range 5.91–6.79). While 7 of 8 PCs-PAS units with platelet yields above 4.0 × 10¹¹/U showed a pH value below 6.8 (range 6.31–6.70). Conclusion: During 7 days of storage in a new 1.3-liter platelet container, the pH was maintained above 6.8 in PCs in plasma with a yield between 1.5- and 4.5 × 10¹¹/U; when PAS II was used, the maximum platelet yield allowed for comparable pH maintenance was somewhat lower (4.0 × 10¹¹/U).

“…A number of studies have shown that when stored in CLX bags for 5-7 days, random donor platelets are viable [1,2] and effective after transfusion [3,4], Preservation of platelet viability during storage re quires that containers be sufficiently permeable to oxygen and carbon dioxide to adequately support aerobic metab olism [5]. When hypoxic conditions develop within the storage container, lactic acid production is stimulated leading to a drop in concentrate pH [6] and, in turn, causing decreased poststorage platelet viability and func tion [7], The number of platelets in a concentrate is the most important variable determining requirements of gas transfer during storage [7,8], Random donor concen trates typically contain on the order of 0.55x 10" plate lets in 40-70 ml plasma (75% of units). Single-donor apheresis concentrates, on the other hand, contain pH and function of stored single-donor platelet concen trates collected on the Haemonetics V-50.…”

Section: Introductionmentioning

confidence: 99%

Extended Storage of Single-Donor Apheresis Platelets in CLX® Blood Bags: Effect of Storage on Platelet Morphology, Viability and in vitro Function

Kenney¹,

Peterson²,

Smith³

1988

A CLX® (Cutter Laboratories, Berkeley, Calif.) bag system was evaluated for storage of single-donor apheresis platelets collected with the Haemonetics V-50 blood processor. Concentrates (n = 21) containing 3.9- 5.2x10^11 platelets in 292 (±41.8) ml were stored in two 1 -liter bags for 7 days at 22 °C. pH was well maintained, declining from an initial pH of 7.0 (±0.04) to 6.92 (±0.20) after 7 days. Platelet morphology, response to a hypotonic stimulus and aggregation induced by paired agonists (epinephrine and ADP, or collagen) were also well-preserved. Concentrates with a wide variation of platelet yields (2.0 ≥ 5.2x10^11, n = 43) also maintained pH (6.96±0.26), morphology and aggregation when stored for 7 days. All platelet concentrates (n=64) were sterile at collection. Single-donor apheresis platelets may be stored in this bag system for up to 7 days.