Platelets Stored in a New-Generation ContainerDifferences between Plasma and Platelet Additive Solution II

Abstract

Background and Objectives: In order to preserve platelet concentrates (PCs) with high yields, a new polyolefin platelet storage container (PL 2410, 1.3L, Baxter, La Châtre, France) with increased gas permeability in combination with a larger surface area has been developed. The storage capacity was studied with platelets in plasma and platelets additive solution. Materials and Methods: Platelet concentrate pools (PCs) of different yields suspended in either plasma (PCs-PL; n = 30) or PAS II (PCs-PAS; n = 37) were prepared. For preparation of PCs with a low, intermediate and high number of platelets 3, 5 and 6 buffy coat (BCs) were pooled with different volumes of plasma and 5 and 6 BCs were pooled with different volumes of PAS II, in order to obtain PCs of equal volumes comparable with routine conditions. All PCs were stored on a flatbed shaker at 22±2 °C and evaluated on days 1, 2, 5 and 7 by determining platelet and white cell counts, pH (37 °C), pO₂, pCO₂ and swirling score. Results: Platelet yields ranged from 1.5 up to 5.5 × 10¹¹ platelets/U. On day 7 all PCs-PL (n = 4) with platelet yields above 4.5 × 10¹¹/U had a pH value below 6.8 (range 5.91–6.79). While 7 of 8 PCs-PAS units with platelet yields above 4.0 × 10¹¹/U showed a pH value below 6.8 (range 6.31–6.70). Conclusion: During 7 days of storage in a new 1.3-liter platelet container, the pH was maintained above 6.8 in PCs in plasma with a yield between 1.5- and 4.5 × 10¹¹/U; when PAS II was used, the maximum platelet yield allowed for comparable pH maintenance was somewhat lower (4.0 × 10¹¹/U).

Evaluation of White Blood Cell- and Platelet-Derived Cytokine Accumulation in MIRASOL-PRT-Treated Platelets

“…Additionally, the role of the storage medium used must also be taken into account when discussing cell lysis parameters. Studies comparing PAS III to 100% plasma, the storage medium of our study, showed higher levels of CCL5 (RANTES), CXCL4 (PLT factor 4), TGF-β1, and PLT activation markers when PLTs were stored in PAS III [31,35] or PAS II [36] rather than 100% plasma, indicating that suspension in 100% plasma protects PLTs from lysis. WBC-associated cytokines (Th-1/2) IL-2, pg/ml IL-4, pg/ml IL-5, pg/ml IL-10, pg/ml TNF, pg/ml IFN-γ, pg/ml 4.37 ± Although it is well established that the frequency of febrile non-hemolytic transfusion reactions is reduced by prestorage leukoreduction, other transfusion reactions, predominantly allergic, still occur frequently [6][7][8].…”