The quality of platelets after storage for 7 days

Evidence-Based Platelet Transfusion Guidelines

“…Comparative studies have shown no difference in the quality of these plt concentrates when they are stored for up to 7 days. 3,4 However, there is emerging evidence that, when plts are stored for extended time periods, the method of plt collection and the storage media influence post-transfusion plt viability. [5][6][7] The hard-spinning of the plts against a red cell layer in the BC method versus against the bottom of the bag in the PRP method, requiring resuspension of the plts, may induce a collection injury that could potentially compromise the long-term storage of PRP plts compared with BC plts.…”

“…11 There is a substantial increase in costs for singledonor compared with pooled random donor plts. As the quality of apheresis plts is similar to pooled random-donor plt concentrates, 3,4 these two products can be used interchangeably based on availability and cost considerations. 12 …”

Influence of Different Methods Preparation on Platelet Activation in Stored Platelet Concentrates

“…Platelets processed by the buffy coat technique showed less CD63 expression than platelets prepared by the PCs method. This study shows that in addition to pH, volume and cell counts; in vitro platelet activation markers were used to monitor platelet [3,13,14]. These data suggest that measurement of CD63, Annexin V and LDH may be a more desirable markers for clinical studies of activated platelets, since it may be less susceptible to artifactual elevation due to minor variations in sample handling and assay [3,12,14].…”

“…Activation of platelet is followed by an increased CD63 expression; this protein secreted from the granules during storage [2,[12][13][14]. Lactatedehydrogenase (LDH), a marker of disintegration of platelets as a parameter for activation marker of platelet concentrates during storage.…”

Platelet Activation in Stored Platelet Concentrates: Comparision of Two Methods Preparation

“…The present paper describes an in vitro comparison of both preparations. The studies suggested that the degree of in vitro activation as evidenced by the expression of CD62P and Annexin V binding were dependent on the different preparative methods [4][5][6][7][8][9][10][11][12][13][14][15][16]17,18]. We found that the extent of activation was significantly higher in PRP-PCs than in BCs.…”