Extended Storage of Single-Donor Apheresis Platelets in CLX® Blood Bags: Effect of Storage on Platelet Morphology, Viability and in vitro Function

Abstract

A CLX® (Cutter Laboratories, Berkeley, Calif.) bag system was evaluated for storage of single-donor apheresis platelets collected with the Haemonetics V-50 blood processor. Concentrates (n = 21) containing 3.9- 5.2x10^11 platelets in 292 (±41.8) ml were stored in two 1 -liter bags for 7 days at 22 °C. pH was well maintained, declining from an initial pH of 7.0 (±0.04) to 6.92 (±0.20) after 7 days. Platelet morphology, response to a hypotonic stimulus and aggregation induced by paired agonists (epinephrine and ADP, or collagen) were also well-preserved. Concentrates with a wide variation of platelet yields (2.0 ≥ 5.2x10^11, n = 43) also maintained pH (6.96±0.26), morphology and aggregation when stored for 7 days. All platelet concentrates (n=64) were sterile at collection. Single-donor apheresis platelets may be stored in this bag system for up to 7 days.