Factors affecting efficient in vitro micropropagation of Muscari muscarimi Medikus using twin bulb scale

Abstract

Endemic Muscari muscarimi Medikus is the most fragrant plant among Muscari species and has a high ornamental potential. The natural populations of M. muscarimi, are severely affected by increased environmental pollution and urbanization. There is a need to develop a micropropagation method that should serve effectively for commercial propagation and conservation. Therefore, the study targeted to set up a strategy for efficient in vitro bulblet regeneration system of M. muscarimi using twin scale bulb explants on 1.0 × MS medium containing 4.44, 8.88, 17.76 μM BAP (6-Benzylaminopurine) plus 2.685, 5.37, 10.74 μM NAA (α-Naphthalene acetic acid). Maximum number of 19 daughter axillary bulblets and 16 daughter adventitious bulblets per twin bulb scale explant was regenerated on 1.0 × MS medium containing 17.76 μM BAP plus 10.74 μM NAA and 17.76 μM BAP plus 2.685 μM NAA respectively. The daughter bulblets regenerated on twin bulb scales on 8 out of 9 regeneration treatment could be easily rooted on 1.0 × MS medium containing 4.9 μM IBA (Indole-3-butyric acid). The daughter bulblets regenerated on 9th treatment (1.0 × MS medium containing 17.76 μM BAP plus 10.74 μM NAA) were transferred to 1.0 × MS medium containing 30 g/l sucrose to break negative carry over effect of this dose of BAP-NAA, where they grew 2-3 roots of variable length. Daughter bulblet diameter was increased by culturing them on 1.0 × MS medium containing 4.44 μM BAP plus 5.37 μM NAA. The results verified that both age and the source of explants had significant effect on regeneration. In another set of experiments, twin scales were obtained from in vitro regenerated daughter bulblets, although they induced bulblets, yet their bulblet regeneration percentage, mean number of bulblets per explant and their diameter were significantly reduced. In vitro regenerated bulblets were acclimatized in growth chamber under ambient conditions of temperature and humidity on peat moss, where they flowered. The study provides important information about selection of suitable micropropagation medium, strategies to improve bulblet diameter and rooting of M. muscarimi which offers a scope for commercial propagation.

Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid

“…The results of the study suggest that the indvidual regeneration on TDZ-NAA-containing MS medium was strongly influenced by the concentrations and combinations of plant growth regulators. The results are in agreement with Uranbey (2010 a, b), Uranbey et al (2011), Ozel et al (2007, 2009, 2015, Vaziri et al (2014) and Uzun et al (2014), who reported the propagation of other Turkish Muscari species using different cytokinins and auxin concentrations.…”

“…It was also noted that MS medium (control) used singly was ineffective to induce roots. These results are not in agreement with Uranbey (2010a,b), Uranbey et al (2010), Vaziri et al (2014), Ozel et al (2007Ozel et al ( , 2009Ozel et al ( , 2015 and Uzun et al (2014)'s studies. The authors used either NAA or IBA for the rooting of different species of Muscari.…”

Enhanced micropropagation protocol of Morinda citrifolia L. through nodal explants

“…Subramani et al (2007) observed rhizogenic calli on MS media with the combination of cytokinin and auxin. Use of IBA as better growth regulator for in vitro root induction has been reported by various researchers in many plant species (Ganesh and Sreenath, 2000;Kavitha et al, 2009;Saha et al, 2010;Nair et al, 2012;Rathore et al, 2014Rathore et al, , 2015Ozel et al, 2015). The rooting Note: Mean separation was analyzed by ANOVA using SPSS software (var.…”

Plant Regeneration Via Somatic Embryogenesis from Leaf Explants ofMuscari Armeniacum

“…As the seeds are not always true to type, plants from seeds grow very slowly. It takes about 3-5 years to mature and flower under natural conditions (11).…”