Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid

Abstract

Muscari neglectum Guss. Ex Ten, is an ornamental, herbaceous perennial plant species that grows in the Mediterranean countries with attractive and scented blue-colored flowers. The plant has low seed output, seed dormancy, low germination and propagation rates. This study aimed to develop a reliable microclonal propagation protocol for M. neglectum using TDZ (Thidiazuron)-NAA (α Naphthalene acetic acid) to induce bulblets, roots, and acclimatization of the regenerated bulblets. Maximum number of bulblets per explant (8.25±0.05) was noted on MS medium containing 0.0454 µM TDZ-5.37 µM NAA. The bulblets regenerated in each type of culture medium were very vigorous, and acclimatized easily following rooting on a subculture. Here we show that this protocol is a useful clonal micropropagation system for this important ornamental plant.

In Vitro Regeneration of Muscari racemosum Mill. Using Twin Bulb Scales, Primary Bulbs, and Leaf Bases

“…Both explant source and plant growth regulators used are very critical factors in plant regeneration and multiplication. Explant sources such as embryonic calli, 2-5 bulb scales, basal layer of the bulbs, leaves, stems, and immature zygotic embryos have been used to stimulate regeneration in several species of genus Muscari (Ozel et al, 2007;Ozel, 2008;Ozel et al, 2009;Vaziri et al, 2009;Uranbey, 2010a, b;Uranbey et al, 2010;Nasırcılar et al, 2011;Uzun et al, 2014;Ozel et al, 2015;Yücesan et al, 2014;Ozel et al, 2016;Özdemir et al, 2017 andFida, 2020). However, there is a need to optimize the ratio of auxin, cytokinin, and other plant growth regulators for regeneration.…”