2013
DOI: 10.5504/bbeq.2013.0092
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Plant Regeneration Via Somatic Embryogenesis from Leaf Explants ofMuscari Armeniacum

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Cited by 15 publications
(7 citation statements)
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“…The action of four potentially selective substances for transformed cells, antibiotics (geneticin, kanamycin, and hygromycin), bialaphos, antibiotics for eliminating Agrobacterium (cefotaxime and carbenicillin) on somatic embryogenesis, and growth of M. armeniacum ("Blue Pearl") calli was evaluated [68]. Furthermore, the system of plant regeneration from M. armeniacum leaf explants through somatic embryogenesis was presented [70], as well as in vitro propagation Bokov (regeneration) systems for this plant [71,72]. Plantlet regeneration Muscari species ("Blue Pearl") from protoplasts was successfully performed [73].…”
Section: Resultsmentioning
confidence: 99%
“…The action of four potentially selective substances for transformed cells, antibiotics (geneticin, kanamycin, and hygromycin), bialaphos, antibiotics for eliminating Agrobacterium (cefotaxime and carbenicillin) on somatic embryogenesis, and growth of M. armeniacum ("Blue Pearl") calli was evaluated [68]. Furthermore, the system of plant regeneration from M. armeniacum leaf explants through somatic embryogenesis was presented [70], as well as in vitro propagation Bokov (regeneration) systems for this plant [71,72]. Plantlet regeneration Muscari species ("Blue Pearl") from protoplasts was successfully performed [73].…”
Section: Resultsmentioning
confidence: 99%
“…Six different stages of buds from male sterile and fertile plants were collected and immersed into fresh FAA (5 mL formalin, 5 mL acetic acid, and 90 mL 70% ethanol) solution, and then kept in an ice box. Paraffin sections were made as previously described (Wang et al 2013 ), The slice thickness is 8 µm, using a Leica RM2016 microtome (Leica, Germany).…”
Section: Methodsmentioning
confidence: 99%
“…armeniacum также является хорошей моделью для получения каллусных культур с эффективной системой генетической трансформации [26,27]. Также была разработана система регенерации растений из эксплантатов листьев M. armeniacum, опосредованная через прямой и непрямой соматический эмбриогенез [28], способ размножения in vitro для этого растения [29]. Луковицы M. armeniacum были предметом исследования гормональной регуляции гуммоза (выделения камедей).…”
Section: рисunclassified