Fine mapping of a male sterility gene ms-3 in a novel cucumber (Cucumis sativus L.) mutant

Han, Yike; Zhao, Fengyue; Gao, Shang; Wang, Xianyun; Wei, Aimin; Chen, Zhengwu; Liu, Nan; Tong, Xueqiang; Fu, Xinmeng; Wen, Changlong; Zhang, Zhenxian; Wang, Ningning; Du, Sheng

doi:10.1007/s00122-017-3013-2

Cited by 30 publications

(18 citation statements)

References 54 publications

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“…The mechanisms underlying crop male sterility can be very complex. Many genetic factors, as well as hormones, photoperiod, temperature, and various forms of stress (Takeno et al, 1996;Kobayasi and Atsuta, 2010;Han et al, 2018), along with a perturbation to carbohydrate supply, can affect anther development, thereby leading to male sterility. As an example, we previously mapped a male sterility gene, ms-3, in a cucumber mutant that is defective in microsporogenesis (Han et al, 2018).…”

Section: Carbon Undersupply Is the Main Cause For Male Sterilitymentioning

confidence: 99%

“…The utilization of male sterility is of importance for hybrid seed production. Many mutations leading to male gametophyte sterility have been shown to interfere with tapetal programmed cell death, meiosis, and mitosis, etc., supporting pivotal roles for these processes in the production of functional pollen grains (Niu et al, 2013;Ko et al, 2014;Han et al, 2018). Abnormal meiosis in the naked oat (Avena nuda) mutant zbs1 also resulted in male sterility by forming defective callose walls and cell plates Yang et al, 2016).…”

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confidence: 99%

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Down-regulation of the Sucrose Transporter CsSUT1 Causes Male Sterility by Altering Carbohydrate Supply

et al. 2019

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ORCID ID: 0000-0002-9247-7185 (Z.Z.).In plants, male sterility is an important agronomic trait, especially in hybrid crop production. Many factors are known to affect crop male sterility, but it remains unclear whether Suc transporters (SUTs) participate directly in this process. Here, we identified and functionally characterized the cucumber (Cucumis sativus) CsSUT1, a typical plasma membrane-localized energy-dependent high-affinity Suc-H + symporter. CsSUT1 is expressed in male flowers and encodes a protein that is localized primarily in the tapetum, pollen, and companion cells of the phloem of sepals, petals, filaments, and pedicel. The male flowers of CsSUT1-RNA interference (RNAi) lines exhibited a decrease in Suc, hexose, and starch content, relative to those of the wild type, during the later stages of male flower development, a finding that was highly associated with male sterility. Transcriptomic analysis revealed that numerous genes associated with sugar metabolism, transport, and signaling, as well as with auxin signaling, were down-regulated, whereas most myeloblastosis (MYB) transcription factor genes were up-regulated in these CsSUT1-RNAi lines relative to wild type. Our findings demonstrate that male sterility can be induced by RNAi-mediated down-regulation of CsSUT1 expression, through the resultant perturbation in carbohydrate delivery and subsequent alteration in sugar and hormone signaling and up-regulation of specific MYB transcription factors. This knowledge provides a new approach for bioengineering male sterility in crop plants.

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Section: Carbon Undersupply Is the Main Cause For Male Sterilitymentioning

confidence: 99%

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confidence: 99%

Down-regulation of the Sucrose Transporter CsSUT1 Causes Male Sterility by Altering Carbohydrate Supply

et al. 2019

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“…The phenomenon of nuclear male sterile caused by point mutation has been reported in cucumber [28] , maize [29] and other crops, but has not been reported in sweet pepper.In this study, the the mutation site that caused the abortion of sweet pepper male sterile AB91, which is on chromosome #5, was first discovered through WGS technology. Flight mass spectrometry genotyping was used to verify the differential gene and filter out the gene Capana05g000747, which is most likely to be the important gene leading to the abortion of sweet pepper male sterile AB91.…”

Section: Capana05g000747 Is An Important Candidate Gene Of Msc2mentioning

confidence: 98%

Identification of Nucleus Sterility Candidate Genes using a Resequencing Technique in Sweet Pepper Sterile Line AB91

Fan

Meng

Yan

et al. 2020

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Breeding hybrids with nuclear male sterile lines is an important method for the cross breeding of sweet peppers. To date, few reports have been published on the nuclear male sterility gene of sweet pepper. Yet, there are approximately 20 pepper nuclear male sterility lines in the world. Using the self-developed testing material, sweet pepper nuclear male sterile dual-purpose line AB91, the genome-wide resequencing technique was applied to firstly find that the mutation site causing the abortion of sweet pepper nuclear male sterility AB91 is on chromosome #5. The mutation gene Capana05g000747 was filtered out and validated by the flight mass spectrometry genotyping method and determined to be the gene causing the abortion of sweet pepper nuclear male sterility AB91. The gene Capana05g000747 contains eight exons and seven introns, and its mutation site is a non-synonymous mutation site located at the 6th exon; the base C mutated into A, and the amino acid changed from alanine to serine. Sequence alignment analysis showed that the gene Capana05g000747 has a similar function to gene At2g02148. The gene At2g02148 contains a pentatricopeptide repeat protein which has important physiological functions in the gene expression process of organelles and is closely related to the performance of male sterility genes. Therefore, Capana05g000747 was selected as an important candidate gene for sweet pepper nuclear male sterile testing material AB91.

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“…The phenomenon of nuclear male sterile caused by point mutation has been reported in cucumber [28], maize [29] and other crops, but has not been reported in sweet pepper.In this study, the the mutation site that caused the abortion of sweet pepper male sterile AB91, which is on chromosome #5, was rst discovered through WGS technology. Flight mass spectrometry genotyping and quantitative real-time PCR were used to verify the differential gene and lter out the gene Capana05g000747, which is most likely to be the important gene leading to the abortion of sweet pepper male sterile AB91.…”

Section: Capana05g000747 Is An Important Candidate Gene Of Msc2mentioning

confidence: 98%

Identification of Nucleus Sterility Candidate Genes using a Resequencing Technique in Sweet Pepper Sterile Line AB91

Meng

Yan

Zhang

et al. 2020

Preprint

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Background:Breeding hybrids with nuclear malesterile lines is an important method for the cross breeding of sweet peppers. To date, few reports have been published on the nuclear malesterility gene of sweet pepper.Yet, there are approximately 20 pepper nuclear malesterility lines in the world. Results: Using the self-developed testing material, sweet pepper nuclear malesterile dual-purpose line AB91, the genome-wide resequencing technique was applied to firstly find that the mutation site causing the abortion of sweet pepper nuclear malesterility AB91 is on chromosome #5. The mutation gene Capana05g000747 was filtered out and validated by the flight mass spectrometry genotyping and quantitative real-time PCR method and determined to be the gene causing the abortion of sweet pepper nuclear male sterility AB91.The gene Capana05g000747 mutation site is a non-synonymous mutation site located at the 6th exon,the base C mutated into A, and the amino acid changed from alanine to serine. The three-dimensional protein structure of fertile and sterile plant Capana05g000747 was predicted. The results showed that the three-dimensional structure of the two proteins was differed significantly. Sequence alignment analysis showed that the gene Capana05g000747 has a similar function to gene At2g02148. The gene At2g02148 contains a pentatricopeptide repeatprotein which has important physiological functions in the gene expression process of organelles and is closely related to the performance of malesterility genes. Conclusions: This study revealed that Capana05g000747 was selected as an important candidate gene for sweet pepper nuclear male sterile testingmaterial AB91.This gene provides a theoretical and technical foundation for further cloning as well as transformation and utilization of the gene.

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Fine mapping of a male sterility gene ms-3 in a novel cucumber (Cucumis sativus L.) mutant

Cited by 30 publications

References 54 publications

Down-regulation of the Sucrose Transporter CsSUT1 Causes Male Sterility by Altering Carbohydrate Supply

Down-regulation of the Sucrose Transporter CsSUT1 Causes Male Sterility by Altering Carbohydrate Supply

Identification of Nucleus Sterility Candidate Genes using a Resequencing Technique in Sweet Pepper Sterile Line AB91

Identification of Nucleus Sterility Candidate Genes using a Resequencing Technique in Sweet Pepper Sterile Line AB91

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