Enhanced micropropagation protocol of Morinda citrifolia L. through nodal explants

Shekhawat, Mahipal S.; Kannan, N.; Manokari, M.; Ravindran, C. P.

doi:10.1016/j.jarmap.2015.06.002

Cited by 24 publications

(25 citation statements)

References 35 publications

(39 reference statements)

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“…1e, f). The present results are totally different from our earlier work on Turnera ulmifolia and Morinda coreia (Shekhawat et al , 2015a where liquid medium was found excellent in multiplication of the shoots in cultures.…”

Section: Multiplication Of Shoots and Maintenance Of Culturescontrasting

confidence: 99%

“…Cyunel (1989) established cultures for the production of pigments using B. alba cells. Effectiveness of BAP over Kin for shoot initiation from the buds has been reported in number of plant species like Ceropegia bulbosa (Phulwaria et al 2013), Leptadenia reticulata , Turnera ulmifolia and Morinda coreia (Shekhawat et al , 2015a.…”

Section: Selection Of Explants and Establishment Of Culturesmentioning

confidence: 99%

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Optimization of in vitro and ex vitro regeneration and micromorphological studies in Basella alba L.

Shekhawat¹,

Manokari²

2016

Physiol Mol Biol Plants

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The optimum concentrations of the plant hormones for in vitro regeneration and subsequent effect of auxins on rooting (in vitro and ex vitro) of shoots of L. have been investigated in present study. Nodal shoot segments were used as explants to initiate the cultures. The bud breaking from explants was observed within 1 week of incubation on agar gelled Murashige and Skoog's (MS) medium. Multiple axillary shoots (7.30 ± 0.56 shoots per explant) were induced on MS medium supplemented with 2.0 mg/L 6-benzylaminopurine (BAP). The shoots were multiplied (maximum 17.10 ± 0.44 shoots per explant) on the same medium fortified with 0.5 mg/L each of BAP and Kin (Kinetin) +0.1 mg/L IAA. These shoots were excised and rooted in vitro (10.73 ± 0.92 roots per shoot) on half-strength MS medium augmented with 2.0 mg/L indole-3 butyric acid (IBA). Hundred percentage success rates have been achieved by ex vitro rooting of the in vitro regenerated shoots with IBA at 300 mg/L. The in vitro and ex vitro rooted shoots were acclimatized in greenhouse and subsequently transferred to the natural field conditions where 100 % survival rate was reported. The ex vitro rooting method was found more advantageous than in vitro rooting in terms of time, energy and survival percentage of. A comparative foliar micromorphological study of was conducted to understand the micromorphological changes in plants while shifting from in vitro to the in vivo conditions in terms of variations in stomatal index, venation pattern and vein density, and the arrangement of crystals. The study could help in understanding the response of in vitro raised plants towards in vivo environment.

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Section: Multiplication Of Shoots and Maintenance Of Culturescontrasting

confidence: 99%

Section: Selection Of Explants and Establishment Of Culturesmentioning

confidence: 99%

Optimization of in vitro and ex vitro regeneration and micromorphological studies in Basella alba L.

Shekhawat¹,

Manokari²

2016

Physiol Mol Biol Plants

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“…However, MS medium containing both auxins and cytokinin was reported less effective in bud break and induction of multiple shoots from the apical and nodal meristems. The effectiveness of BAP over Kin in bud breaking and shoot proliferation has been well documented in several plant species (Singh and Tiwari 2012;Shekhawat et al 2015b;Patel et al 2014). The rejuvenation of meristem was achieved in plants by selecting the age of the mother plant, nature of explant, season of explant collection, plant growth regulators and additives (ascorbic and citric acids, adenine sulphate and arginine) used (Arya et al 2002).…”

Section: Resultsmentioning

confidence: 99%

“…It has been reported that ex vitro rooted plants are better suited to tolerate environmental stresses (Baskaran and Van Staden 2013). Shekhawat et al (2014Shekhawat et al ( , 2015b have successfully achieved ex vitro root induction in Melothria maderaspatana, Turnera ulmifolia and Morinda citrifolia. It is a cost effective technique which could save labor, time and energy in in vitro plant propagation system (Ranaweeraa et al 2013).…”

Section: Ex Vitro Rooting and Acclimatization Of Plantletsmentioning

confidence: 99%

In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis aubl.): a multipurpose threatened species

Shekhawat¹,

Manokari²

2016

Physiol Mol Biol Plants

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“…Maximum 45.0 ± 0.48 roots per shoot were observed during this experiment (Table 5). Baskaran et al (2009) and Shekhawat et al (2015b) have successfully achieved ex vitro root induction in Melothria maderaspatana and Morinda citrifolia, respectively. Ex vitro rooting ensure better hardening and improved survival rate than the in vitro rooting method (Dhavala and Rathore 2010).…”

Section: Ex Vitro Root Induction From the Shoots In Greenhousementioning

confidence: 99%

In vitro regeneration frequency, micro-morphological studies and ex vitro rooting of Hemidesmus indicus (L.) R. Br.: a multi-potent endangered climber

Shekhawat¹,

Manokari²

2016

Ind J Plant Physiol.

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Hemidesmus indicus is an endangered medicinal plant of Peninsular India. A rapid micropropagation method using nodal segments of a 4 year old plant has been achieved in the present study. The nodal explants (2.0-3.0 cm long) were harvested from actively growing shoots of conventionally raised plants and cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg l -1 6-benzylaminopurine (BAP). Hundred percent explants responded with 5.2 ± 0.25 cm long multiple shoots (9.0 ± 0.53) from the nodal meristems. The shoots were further multiplied enormously (272 ± 4.12 shoots per explant) by repeated subculture of mother explants and freshly induced shoot clumps on MS medium with the reduced concentrations of BAP (1.0 mg l -1 ), kinetin (Kin; 0.5 mg l -1 ) and indole-3 acetic acid (IAA; 0.1 mg l -1 ) within 4 weeks. Highest root induction (62.0 ± 0.54 roots per shoot) was observed on 1/4th strength MS medium supplemented with 3.0 mg l -1 indole-3 butyric acid (IBA). About 96 % shoots were rooted (45.0 ± 0.48 roots per shoot) by ex vitro methods when the cut ends of the shoots pulse treated with IBA (400 mg l -1 ) for 5 min. The in vitro as well as ex vitro rooted plantlets were acclimatized successfully in the greenhouse. Ex vitro rooted plantlets exhibited higher percentage (98 %) of survival as compared to the in vitro rooted plantlets (91 %) in the field conditions. There were not any observable differences between in vitro propagated and field transplanted plantlets. A comparative foliar micro-morphological study of H. indicus was conducted to understand the micromorphological changes in the plants while shifting from in vitro to the in vivo conditions in terms of variations in stomata, venation pattern and vein density, and trichomes. The aforementioned protocol could be successfully used for the large-scale multiplication and conservation of germplasm of this endangered plant species.

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Enhanced micropropagation protocol of Morinda citrifolia L. through nodal explants

Cited by 24 publications

References 35 publications

Optimization of in vitro and ex vitro regeneration and micromorphological studies in Basella alba L.

Optimization of in vitro and ex vitro regeneration and micromorphological studies in Basella alba L.

In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis aubl.): a multipurpose threatened species

In vitro regeneration frequency, micro-morphological studies and ex vitro rooting of Hemidesmus indicus (L.) R. Br.: a multi-potent endangered climber

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